Karyological analysis of Cyprinion macrostomum Heckel, 1843, from Godarkhosh River, Ilam Province, Iran
In this study, for the first time in Iran, the karyotype of bigmouth Lotak, Cyprinion macrostomum Heckel, 1843, was investigated through examining metaphase chromosomes of seven fish with mean weight 30±5g caught by electrofishing from Godarkhosh River in Ilam Province. To stimulate cell divisions, fish were injected intraperitoneally two times by phytohemagglutinin (PHA). The cell divisions were arrested in metaphase stage by intraperitoneal injection of colchicine. Well-separated cells were obtained from kidney and gill filament and chromosome spreads were prepared and stained with giemsa. Karyotype was obtained as 2n=50. The karyotype consisted of 5 metacentric, 12 submetacentric and 8 telocentric chromosome pairs. Centromeric index, arm ratio and Fundamental Number (FN) were determined as 0-50, 1-∞, and 84, respectively.
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2015
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Subjects: | Biology, Bigmouth lotak, Cyprinion macrostomum, Godarkhosh River, Iran, Karyotype, Physiology, |
Online Access: | http://hdl.handle.net/1834/37566 |
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dig-aquadocs-1834-375662021-07-10T02:40:42Z Karyological analysis of Cyprinion macrostomum Heckel, 1843, from Godarkhosh River, Ilam Province, Iran Nasri, M. Keivany, Y. Dorafshan, S. Biology Bigmouth lotak Cyprinion macrostomum Godarkhosh River Iran Karyotype Biology Physiology In this study, for the first time in Iran, the karyotype of bigmouth Lotak, Cyprinion macrostomum Heckel, 1843, was investigated through examining metaphase chromosomes of seven fish with mean weight 30±5g caught by electrofishing from Godarkhosh River in Ilam Province. To stimulate cell divisions, fish were injected intraperitoneally two times by phytohemagglutinin (PHA). The cell divisions were arrested in metaphase stage by intraperitoneal injection of colchicine. Well-separated cells were obtained from kidney and gill filament and chromosome spreads were prepared and stained with giemsa. Karyotype was obtained as 2n=50. The karyotype consisted of 5 metacentric, 12 submetacentric and 8 telocentric chromosome pairs. Centromeric index, arm ratio and Fundamental Number (FN) were determined as 0-50, 1-∞, and 84, respectively. 2021-06-24T18:06:30Z 2021-06-24T18:06:30Z 2015 article TRUE 1562-2916 http://hdl.handle.net/1834/37566 en http://www.ifro.ir application/pdf application/pdf 786-796 http://aquaticcommons.org/id/eprint/22816 18721 2018-05-24 21:52:12 22816 Iranian Fisheries Science Research Institute |
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Biology Bigmouth lotak Cyprinion macrostomum Godarkhosh River Iran Karyotype Biology Physiology Biology Bigmouth lotak Cyprinion macrostomum Godarkhosh River Iran Karyotype Biology Physiology |
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Biology Bigmouth lotak Cyprinion macrostomum Godarkhosh River Iran Karyotype Biology Physiology Biology Bigmouth lotak Cyprinion macrostomum Godarkhosh River Iran Karyotype Biology Physiology Nasri, M. Keivany, Y. Dorafshan, S. Karyological analysis of Cyprinion macrostomum Heckel, 1843, from Godarkhosh River, Ilam Province, Iran |
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In this study, for the first time in Iran, the karyotype of bigmouth Lotak, Cyprinion macrostomum Heckel, 1843, was investigated through examining metaphase chromosomes of seven fish with mean weight 30±5g caught by electrofishing from Godarkhosh River in Ilam Province. To stimulate cell divisions, fish were injected intraperitoneally two times by phytohemagglutinin (PHA). The cell divisions were arrested in metaphase stage by intraperitoneal injection of colchicine. Well-separated cells were obtained from kidney and gill filament and chromosome spreads were prepared and stained with giemsa. Karyotype was obtained as 2n=50. The karyotype consisted of 5 metacentric, 12 submetacentric and 8 telocentric chromosome pairs. Centromeric index, arm ratio and Fundamental Number (FN) were determined as 0-50, 1-∞, and 84, respectively. |
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article |
topic_facet |
Biology Bigmouth lotak Cyprinion macrostomum Godarkhosh River Iran Karyotype Biology Physiology |
author |
Nasri, M. Keivany, Y. Dorafshan, S. |
author_facet |
Nasri, M. Keivany, Y. Dorafshan, S. |
author_sort |
Nasri, M. |
title |
Karyological analysis of Cyprinion macrostomum Heckel, 1843, from Godarkhosh River, Ilam Province, Iran |
title_short |
Karyological analysis of Cyprinion macrostomum Heckel, 1843, from Godarkhosh River, Ilam Province, Iran |
title_full |
Karyological analysis of Cyprinion macrostomum Heckel, 1843, from Godarkhosh River, Ilam Province, Iran |
title_fullStr |
Karyological analysis of Cyprinion macrostomum Heckel, 1843, from Godarkhosh River, Ilam Province, Iran |
title_full_unstemmed |
Karyological analysis of Cyprinion macrostomum Heckel, 1843, from Godarkhosh River, Ilam Province, Iran |
title_sort |
karyological analysis of cyprinion macrostomum heckel, 1843, from godarkhosh river, ilam province, iran |
publishDate |
2015 |
url |
http://hdl.handle.net/1834/37566 |
work_keys_str_mv |
AT nasrim karyologicalanalysisofcyprinionmacrostomumheckel1843fromgodarkhoshriverilamprovinceiran AT keivanyy karyologicalanalysisofcyprinionmacrostomumheckel1843fromgodarkhoshriverilamprovinceiran AT dorafshans karyologicalanalysisofcyprinionmacrostomumheckel1843fromgodarkhoshriverilamprovinceiran |
_version_ |
1756079641951469568 |