Changes of digestive enzymes activity in Caspian kutum (Rutilus frisii kutum) during larval developmental stages

The ontogenesis and specific activities of pancreatic (trypsin, chymotrypsin, amylase and lipase) and intestinal enzymes (alkaline phosphatase, aminopeptidase N) were investigated in kutum (Rutilus frisii Kutum) from the onset of exogenous feeding (3 day after hatching, DAH) to the juvenile stage at 50 DAH. Trypsin- and chymotrypsin-specific activity showed similar patterns and increased with larval development and age. After the first feeding, specific activity of amylase and lipase increased and reached a peak at 10 DAH, followed by a sharp decrease until 25 DAH, after which it is increased again. Carbohydrate and lipid content changes in diet have led to fluctuations (increases or decreases) in amylase and lipase activities. Alkaline phosphatase and aminopeptidase N specific activity had similar patterns and showed increased trend with age. Sharp increases in activity for both enzymes from 7-10 DAH indicated maturation of the enterocytes and the achievement of adult-like mode of digestion. Our results suggest that kutum is capable of digesting protein, lipid and carbohydrates at early stages of growth. However, due to low level of lipase-specific activity compared to other enzymes, it seems that kutum larvae prefer diets containing higher protein levels than diets with higher lipid content. Therefore, for this species lipid component should remain at low level in formulated diets. In this study, specific activity of most digestive enzymes exhibited a sharp increase when co-feeding on rotifers and formulated diet started from 7-10 DAH. The increase in activities of pancreatic and intestinal enzymes in this period can be due to maturation of exocrine pancreas and brush border enterocytes, respectively and showed that this fish has ability to digest formulated diets.

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Bibliographic Details
Main Authors: Khosravi Bakhtiarvandi, N., Abedian-Kenari, A.M.
Format: article biblioteca
Language:English
Published: 2015
Subjects:Biology, Fisheries, Rutilus frisii Kutum, Larviculture, Pancreatic enzymes, Intestinal enzymes, Ontogeny, Physiology, Caspian Sea, Iran,
Online Access:http://hdl.handle.net/1834/37515
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spelling dig-aquadocs-1834-375152021-07-09T03:29:53Z Changes of digestive enzymes activity in Caspian kutum (Rutilus frisii kutum) during larval developmental stages Khosravi Bakhtiarvandi, N. Abedian-Kenari, A.M. Biology Fisheries Rutilus frisii Kutum Larviculture Pancreatic enzymes Intestinal enzymes Ontogeny Biology Physiology Caspian Sea Iran The ontogenesis and specific activities of pancreatic (trypsin, chymotrypsin, amylase and lipase) and intestinal enzymes (alkaline phosphatase, aminopeptidase N) were investigated in kutum (Rutilus frisii Kutum) from the onset of exogenous feeding (3 day after hatching, DAH) to the juvenile stage at 50 DAH. Trypsin- and chymotrypsin-specific activity showed similar patterns and increased with larval development and age. After the first feeding, specific activity of amylase and lipase increased and reached a peak at 10 DAH, followed by a sharp decrease until 25 DAH, after which it is increased again. Carbohydrate and lipid content changes in diet have led to fluctuations (increases or decreases) in amylase and lipase activities. Alkaline phosphatase and aminopeptidase N specific activity had similar patterns and showed increased trend with age. Sharp increases in activity for both enzymes from 7-10 DAH indicated maturation of the enterocytes and the achievement of adult-like mode of digestion. Our results suggest that kutum is capable of digesting protein, lipid and carbohydrates at early stages of growth. However, due to low level of lipase-specific activity compared to other enzymes, it seems that kutum larvae prefer diets containing higher protein levels than diets with higher lipid content. Therefore, for this species lipid component should remain at low level in formulated diets. In this study, specific activity of most digestive enzymes exhibited a sharp increase when co-feeding on rotifers and formulated diet started from 7-10 DAH. The increase in activities of pancreatic and intestinal enzymes in this period can be due to maturation of exocrine pancreas and brush border enterocytes, respectively and showed that this fish has ability to digest formulated diets. 2021-06-24T18:06:11Z 2021-06-24T18:06:11Z 2015 article TRUE 1562-2916 http://hdl.handle.net/1834/37515 en http://www.ifro.ir application/pdf application/pdf 158-175 http://aquaticcommons.org/id/eprint/22764 18721 2018-05-21 18:21:08 22764 Iranian Fisheries Science Research Institute
institution UNESCO
collection DSpace
country Francia
countrycode FR
component Bibliográfico
access En linea
databasecode dig-aquadocs
tag biblioteca
region Europa del Oeste
libraryname Repositorio AQUADOCS
language English
topic Biology
Fisheries
Rutilus frisii Kutum
Larviculture
Pancreatic enzymes
Intestinal enzymes
Ontogeny
Biology
Physiology
Caspian Sea
Iran
Biology
Fisheries
Rutilus frisii Kutum
Larviculture
Pancreatic enzymes
Intestinal enzymes
Ontogeny
Biology
Physiology
Caspian Sea
Iran
spellingShingle Biology
Fisheries
Rutilus frisii Kutum
Larviculture
Pancreatic enzymes
Intestinal enzymes
Ontogeny
Biology
Physiology
Caspian Sea
Iran
Biology
Fisheries
Rutilus frisii Kutum
Larviculture
Pancreatic enzymes
Intestinal enzymes
Ontogeny
Biology
Physiology
Caspian Sea
Iran
Khosravi Bakhtiarvandi, N.
Abedian-Kenari, A.M.
Changes of digestive enzymes activity in Caspian kutum (Rutilus frisii kutum) during larval developmental stages
description The ontogenesis and specific activities of pancreatic (trypsin, chymotrypsin, amylase and lipase) and intestinal enzymes (alkaline phosphatase, aminopeptidase N) were investigated in kutum (Rutilus frisii Kutum) from the onset of exogenous feeding (3 day after hatching, DAH) to the juvenile stage at 50 DAH. Trypsin- and chymotrypsin-specific activity showed similar patterns and increased with larval development and age. After the first feeding, specific activity of amylase and lipase increased and reached a peak at 10 DAH, followed by a sharp decrease until 25 DAH, after which it is increased again. Carbohydrate and lipid content changes in diet have led to fluctuations (increases or decreases) in amylase and lipase activities. Alkaline phosphatase and aminopeptidase N specific activity had similar patterns and showed increased trend with age. Sharp increases in activity for both enzymes from 7-10 DAH indicated maturation of the enterocytes and the achievement of adult-like mode of digestion. Our results suggest that kutum is capable of digesting protein, lipid and carbohydrates at early stages of growth. However, due to low level of lipase-specific activity compared to other enzymes, it seems that kutum larvae prefer diets containing higher protein levels than diets with higher lipid content. Therefore, for this species lipid component should remain at low level in formulated diets. In this study, specific activity of most digestive enzymes exhibited a sharp increase when co-feeding on rotifers and formulated diet started from 7-10 DAH. The increase in activities of pancreatic and intestinal enzymes in this period can be due to maturation of exocrine pancreas and brush border enterocytes, respectively and showed that this fish has ability to digest formulated diets.
format article
topic_facet Biology
Fisheries
Rutilus frisii Kutum
Larviculture
Pancreatic enzymes
Intestinal enzymes
Ontogeny
Biology
Physiology
Caspian Sea
Iran
author Khosravi Bakhtiarvandi, N.
Abedian-Kenari, A.M.
author_facet Khosravi Bakhtiarvandi, N.
Abedian-Kenari, A.M.
author_sort Khosravi Bakhtiarvandi, N.
title Changes of digestive enzymes activity in Caspian kutum (Rutilus frisii kutum) during larval developmental stages
title_short Changes of digestive enzymes activity in Caspian kutum (Rutilus frisii kutum) during larval developmental stages
title_full Changes of digestive enzymes activity in Caspian kutum (Rutilus frisii kutum) during larval developmental stages
title_fullStr Changes of digestive enzymes activity in Caspian kutum (Rutilus frisii kutum) during larval developmental stages
title_full_unstemmed Changes of digestive enzymes activity in Caspian kutum (Rutilus frisii kutum) during larval developmental stages
title_sort changes of digestive enzymes activity in caspian kutum (rutilus frisii kutum) during larval developmental stages
publishDate 2015
url http://hdl.handle.net/1834/37515
work_keys_str_mv AT khosravibakhtiarvandin changesofdigestiveenzymesactivityincaspiankutumrutilusfrisiikutumduringlarvaldevelopmentalstages
AT abediankenariam changesofdigestiveenzymesactivityincaspiankutumrutilusfrisiikutumduringlarvaldevelopmentalstages
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