Genetic characterization of Vimba vimba persa (Pallas, 1814) in southern parts of the Caspian Sea using microsatellite markers
Population genetic structure of Vimba vimba persa was investigated using microsatellite markers from 40 regions along the Iranian coastline of the Southern Caspian Sea (Anzali lagoon and Havigh River in Gilan province, BabolRoud River in Mazandaran province and GorganRoud River in Golestan province). Genomic DNA from 121 specimens was extracted from fin tissue by the Phenol-Chlorophorm method and PCR reaction was accomplished with 17 microsatellite primers, out of 17 microsatellite primers 13 loci were amplified, in which 10 of them were amplified with reasonable polymorphism and 3 were monomorphism. A total of 302 alleles were identified on average 7.55. Observed and expected heterozygosity averages were 0.80 and 0.77 respectively. Most cases significantly deviated from Hardy-Weinberg equilibrium (p≤0.01). The estimation of Fst (p≤0.01) revealed significant population structuring and an estimation of the four population of Vimba vimba persa was identified in the Caspian Sea in which restocking of these species should be considered.
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Language: | English |
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2012
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Subjects: | Biology, Fisheries, Vimba vimba persa, Population genetic, Microsatellite, Caspian Sea, Iran, |
Online Access: | http://hdl.handle.net/1834/37285 |
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dig-aquadocs-1834-372852021-07-06T03:09:50Z Genetic characterization of Vimba vimba persa (Pallas, 1814) in southern parts of the Caspian Sea using microsatellite markers Mohamadian, S. Rezavani Gilkolaei, S. Rouhollahi, S. Taghavi, M.J. Nayerani, M. Shirzad, E. Taheri Mirghaed, A. Biology Fisheries Vimba vimba persa Population genetic Microsatellite Caspian Sea Iran Population genetic structure of Vimba vimba persa was investigated using microsatellite markers from 40 regions along the Iranian coastline of the Southern Caspian Sea (Anzali lagoon and Havigh River in Gilan province, BabolRoud River in Mazandaran province and GorganRoud River in Golestan province). Genomic DNA from 121 specimens was extracted from fin tissue by the Phenol-Chlorophorm method and PCR reaction was accomplished with 17 microsatellite primers, out of 17 microsatellite primers 13 loci were amplified, in which 10 of them were amplified with reasonable polymorphism and 3 were monomorphism. A total of 302 alleles were identified on average 7.55. Observed and expected heterozygosity averages were 0.80 and 0.77 respectively. Most cases significantly deviated from Hardy-Weinberg equilibrium (p≤0.01). The estimation of Fst (p≤0.01) revealed significant population structuring and an estimation of the four population of Vimba vimba persa was identified in the Caspian Sea in which restocking of these species should be considered. Pages 356 and 357 are misnumbered. Article includes abstract in Farsi on last page. 2021-06-24T18:04:50Z 2021-06-24T18:04:50Z 2012 article TRUE 1562-2916 http://hdl.handle.net/1834/37285 en http://www.ifro.ir application/pdf application/pdf 347-357 http://aquaticcommons.org/id/eprint/22518 18721 2018-04-16 21:57:58 22518 Iranian Fisheries Science Research Institute |
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Biology Fisheries Vimba vimba persa Population genetic Microsatellite Caspian Sea Iran Biology Fisheries Vimba vimba persa Population genetic Microsatellite Caspian Sea Iran |
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Biology Fisheries Vimba vimba persa Population genetic Microsatellite Caspian Sea Iran Biology Fisheries Vimba vimba persa Population genetic Microsatellite Caspian Sea Iran Mohamadian, S. Rezavani Gilkolaei, S. Rouhollahi, S. Taghavi, M.J. Nayerani, M. Shirzad, E. Taheri Mirghaed, A. Genetic characterization of Vimba vimba persa (Pallas, 1814) in southern parts of the Caspian Sea using microsatellite markers |
description |
Population genetic structure of Vimba vimba persa was investigated using microsatellite markers from 40 regions along the Iranian coastline of the Southern Caspian Sea (Anzali lagoon and Havigh River in Gilan province, BabolRoud River in Mazandaran province and GorganRoud River in Golestan province). Genomic DNA from 121 specimens was extracted from fin tissue by the Phenol-Chlorophorm method and PCR reaction was accomplished with 17 microsatellite primers, out of 17 microsatellite primers 13 loci were amplified, in which 10 of them were amplified with reasonable polymorphism and 3 were monomorphism. A total of 302 alleles were identified on average 7.55. Observed and expected heterozygosity averages were 0.80 and 0.77 respectively. Most cases significantly deviated from Hardy-Weinberg equilibrium (p≤0.01). The estimation of Fst (p≤0.01) revealed significant population structuring and an estimation of the four population of Vimba vimba persa was identified in the Caspian Sea in which restocking of these species should be considered. |
format |
article |
topic_facet |
Biology Fisheries Vimba vimba persa Population genetic Microsatellite Caspian Sea Iran |
author |
Mohamadian, S. Rezavani Gilkolaei, S. Rouhollahi, S. Taghavi, M.J. Nayerani, M. Shirzad, E. Taheri Mirghaed, A. |
author_facet |
Mohamadian, S. Rezavani Gilkolaei, S. Rouhollahi, S. Taghavi, M.J. Nayerani, M. Shirzad, E. Taheri Mirghaed, A. |
author_sort |
Mohamadian, S. |
title |
Genetic characterization of Vimba vimba persa (Pallas, 1814) in southern parts of the Caspian Sea using microsatellite markers |
title_short |
Genetic characterization of Vimba vimba persa (Pallas, 1814) in southern parts of the Caspian Sea using microsatellite markers |
title_full |
Genetic characterization of Vimba vimba persa (Pallas, 1814) in southern parts of the Caspian Sea using microsatellite markers |
title_fullStr |
Genetic characterization of Vimba vimba persa (Pallas, 1814) in southern parts of the Caspian Sea using microsatellite markers |
title_full_unstemmed |
Genetic characterization of Vimba vimba persa (Pallas, 1814) in southern parts of the Caspian Sea using microsatellite markers |
title_sort |
genetic characterization of vimba vimba persa (pallas, 1814) in southern parts of the caspian sea using microsatellite markers |
publishDate |
2012 |
url |
http://hdl.handle.net/1834/37285 |
work_keys_str_mv |
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