Induction of tetraploidy in transgenic tilapia (Oreochromis niloticus) using physical shocks
The induction of tetraploidy by means of cold, heat and multiple heat shock treatments was investigated on male fish from a growth-enhanced transgenic tilapia C118 line, crossed with wild type females. After the development a new multiple heat shock protocol (two heat treatments at 41oC in 60 and 80 min. after fertilization for 5 min. per each), chromosome and X-gal in situ staining assays demonstrated the successful production of yolk sac tetraploid transgenic tilapia in two out of 15 embryos examined in this trial via direct correlation between higher level of b-galactosidase expression and karyotyping in the samples verifying complete tetraploidy in this line. In the grown fish, there was evidence to suggest mosaicism or perhaps tetraploidy in at least two grown fish using the measurement of erythrocytes and karyotyping of the G1 offspring (p<0.05). There was, however, no evidence to indicate successful production of triploid G1 individuals following crossing of induced fish with wild type.
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Format: | article biblioteca |
Language: | English |
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2007
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Subjects: | Biology, Fisheries, Transgenic tilapia, Oreochromis niloticus, galactosidase, Tetraploidy, LacZ reporter gene, Sterility, |
Online Access: | http://hdl.handle.net/1834/37090 |
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dig-aquadocs-1834-370902021-07-02T03:12:04Z Induction of tetraploidy in transgenic tilapia (Oreochromis niloticus) using physical shocks Farahmand, H. Abdul Razak, Sh. Hwang, G.L. Rahman, M.A. Maclean, N. Biology Fisheries Transgenic tilapia Oreochromis niloticus galactosidase Tetraploidy LacZ reporter gene Sterility The induction of tetraploidy by means of cold, heat and multiple heat shock treatments was investigated on male fish from a growth-enhanced transgenic tilapia C118 line, crossed with wild type females. After the development a new multiple heat shock protocol (two heat treatments at 41oC in 60 and 80 min. after fertilization for 5 min. per each), chromosome and X-gal in situ staining assays demonstrated the successful production of yolk sac tetraploid transgenic tilapia in two out of 15 embryos examined in this trial via direct correlation between higher level of b-galactosidase expression and karyotyping in the samples verifying complete tetraploidy in this line. In the grown fish, there was evidence to suggest mosaicism or perhaps tetraploidy in at least two grown fish using the measurement of erythrocytes and karyotyping of the G1 offspring (p<0.05). There was, however, no evidence to indicate successful production of triploid G1 individuals following crossing of induced fish with wild type. 2021-06-24T18:03:29Z 2021-06-24T18:03:29Z 2007 article TRUE 1562-2916 http://hdl.handle.net/1834/37090 en http://www.ifro.ir application/pdf application/pdf 27-46 http://aquaticcommons.org/id/eprint/22304 18721 2018-03-14 08:10:36 22304 Iranian Fisheries Science Research Institute |
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Biology Fisheries Transgenic tilapia Oreochromis niloticus galactosidase Tetraploidy LacZ reporter gene Sterility Biology Fisheries Transgenic tilapia Oreochromis niloticus galactosidase Tetraploidy LacZ reporter gene Sterility |
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Biology Fisheries Transgenic tilapia Oreochromis niloticus galactosidase Tetraploidy LacZ reporter gene Sterility Biology Fisheries Transgenic tilapia Oreochromis niloticus galactosidase Tetraploidy LacZ reporter gene Sterility Farahmand, H. Abdul Razak, Sh. Hwang, G.L. Rahman, M.A. Maclean, N. Induction of tetraploidy in transgenic tilapia (Oreochromis niloticus) using physical shocks |
description |
The induction of tetraploidy by means of cold, heat and multiple heat shock treatments was investigated on male fish from a growth-enhanced transgenic tilapia C118 line, crossed with wild type females. After the development a new multiple heat shock protocol (two heat treatments at 41oC in 60 and 80 min. after fertilization for 5 min. per each), chromosome and X-gal in situ staining assays demonstrated the successful production of yolk sac tetraploid transgenic tilapia in two out of 15 embryos examined in this trial via direct correlation between higher level of b-galactosidase expression and karyotyping in the samples verifying complete tetraploidy in this line. In the grown fish, there was evidence to suggest mosaicism or perhaps tetraploidy in at least two grown fish using the measurement of erythrocytes and karyotyping of the G1 offspring (p<0.05). There was, however, no evidence to indicate successful production of triploid G1 individuals following crossing of induced fish with wild type. |
format |
article |
topic_facet |
Biology Fisheries Transgenic tilapia Oreochromis niloticus galactosidase Tetraploidy LacZ reporter gene Sterility |
author |
Farahmand, H. Abdul Razak, Sh. Hwang, G.L. Rahman, M.A. Maclean, N. |
author_facet |
Farahmand, H. Abdul Razak, Sh. Hwang, G.L. Rahman, M.A. Maclean, N. |
author_sort |
Farahmand, H. |
title |
Induction of tetraploidy in transgenic tilapia (Oreochromis niloticus) using physical shocks |
title_short |
Induction of tetraploidy in transgenic tilapia (Oreochromis niloticus) using physical shocks |
title_full |
Induction of tetraploidy in transgenic tilapia (Oreochromis niloticus) using physical shocks |
title_fullStr |
Induction of tetraploidy in transgenic tilapia (Oreochromis niloticus) using physical shocks |
title_full_unstemmed |
Induction of tetraploidy in transgenic tilapia (Oreochromis niloticus) using physical shocks |
title_sort |
induction of tetraploidy in transgenic tilapia (oreochromis niloticus) using physical shocks |
publishDate |
2007 |
url |
http://hdl.handle.net/1834/37090 |
work_keys_str_mv |
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_version_ |
1756079578086899712 |