Optimization of UV irradiation for production of gynogenetic rainbow trout, Oncorhynchus mykiss: emphasising Hertwig effect and photoreactivation
The optimum dose of UV irradiation to produce gynogenesis rainbow trout, Oncorhynchus mykiss with emphasis of Hertwig effect and photoreactivation (PR) was investigated. For this purpose, the sperm of rainbow trout were irradiated with UV at 2010±200 µw.cm-2intensity in different alternatives of 0, 1, 3, 5, 8, 10, 15, 20, 25, 35 and 45 minutes and allowed to fertilize normal ova; the fertilization, eyed and hatching rate were calculated to assess the performance. Using the irradiated sperm decreased the fertilization, eyed as well as hatching rates and the so-called "Hertwig effect" was observed, with the time-dependent decrease in hatching rate at 0+to 10 min of irradiation, but a better hatching rate at greater time of irradiation was observed. The highest hatching rate was observed in 20 min of UV irradiation; after that the survival rate decreased rapidly. Interestingly, irradiation even up to 45 min could not eradicate sperm fertility and a hatching rate above zero was observed at this treatment. For PR studies the semen was irradiated with UV for 5, 30 and 120 seconds and untreated semen (Os) was used as control. Irradiated semen and/or fertilized eggs by treated semen were exposed to visible light (60 W) at a distance of 30 cm for 10 min; the eyed and hatching rates were measured. The results showed that UV irradiation in as low as 5s could cause deleterious effect on semen chromatin and decrease the eyed and hatching rates (p<0.05). Illumination of semen and\or eggs with visible light resulted in PR in rainbow trout when sperm was irradiated by UV at 120s. We could conclude that the best UV irradiation time for production of gynogenesis rainbow trout was 20 min and UV irradiation, fertilization and egg hardening must be done far from visible light to eliminate the PR mechanism.
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Format: | article biblioteca |
Language: | English |
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2006
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Subjects: | Biology, Fisheries, Gynogenesis, Hertwig effect, Oncorhynchus mykiss, Photoreactivation, Rainbow trout, Ultraviolet, Iran irraciation, |
Online Access: | http://hdl.handle.net/1834/37073 |
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dig-aquadocs-1834-370732021-07-02T02:28:04Z Optimization of UV irradiation for production of gynogenetic rainbow trout, Oncorhynchus mykiss: emphasising Hertwig effect and photoreactivation Dorafshan, S. Kalbassi, M.R. Pourkazemi, M. Mojazi Amiri, B. Biology Fisheries Gynogenesis Hertwig effect Oncorhynchus mykiss Photoreactivation Rainbow trout Ultraviolet Iran irraciation The optimum dose of UV irradiation to produce gynogenesis rainbow trout, Oncorhynchus mykiss with emphasis of Hertwig effect and photoreactivation (PR) was investigated. For this purpose, the sperm of rainbow trout were irradiated with UV at 2010±200 µw.cm-2intensity in different alternatives of 0, 1, 3, 5, 8, 10, 15, 20, 25, 35 and 45 minutes and allowed to fertilize normal ova; the fertilization, eyed and hatching rate were calculated to assess the performance. Using the irradiated sperm decreased the fertilization, eyed as well as hatching rates and the so-called "Hertwig effect" was observed, with the time-dependent decrease in hatching rate at 0+to 10 min of irradiation, but a better hatching rate at greater time of irradiation was observed. The highest hatching rate was observed in 20 min of UV irradiation; after that the survival rate decreased rapidly. Interestingly, irradiation even up to 45 min could not eradicate sperm fertility and a hatching rate above zero was observed at this treatment. For PR studies the semen was irradiated with UV for 5, 30 and 120 seconds and untreated semen (Os) was used as control. Irradiated semen and/or fertilized eggs by treated semen were exposed to visible light (60 W) at a distance of 30 cm for 10 min; the eyed and hatching rates were measured. The results showed that UV irradiation in as low as 5s could cause deleterious effect on semen chromatin and decrease the eyed and hatching rates (p<0.05). Illumination of semen and\or eggs with visible light resulted in PR in rainbow trout when sperm was irradiated by UV at 120s. We could conclude that the best UV irradiation time for production of gynogenesis rainbow trout was 20 min and UV irradiation, fertilization and egg hardening must be done far from visible light to eliminate the PR mechanism. 2021-06-24T18:03:18Z 2021-06-24T18:03:18Z 2006 article TRUE 1562-2916 http://hdl.handle.net/1834/37073 en http://www.ifro.ir application/pdf application/pdf 19-34 http://aquaticcommons.org/id/eprint/22287 18721 2018-03-13 02:18:42 22287 Iranian Fisheries Science Research Institute |
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Biology Fisheries Gynogenesis Hertwig effect Oncorhynchus mykiss Photoreactivation Rainbow trout Ultraviolet Iran irraciation Biology Fisheries Gynogenesis Hertwig effect Oncorhynchus mykiss Photoreactivation Rainbow trout Ultraviolet Iran irraciation |
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Biology Fisheries Gynogenesis Hertwig effect Oncorhynchus mykiss Photoreactivation Rainbow trout Ultraviolet Iran irraciation Biology Fisheries Gynogenesis Hertwig effect Oncorhynchus mykiss Photoreactivation Rainbow trout Ultraviolet Iran irraciation Dorafshan, S. Kalbassi, M.R. Pourkazemi, M. Mojazi Amiri, B. Optimization of UV irradiation for production of gynogenetic rainbow trout, Oncorhynchus mykiss: emphasising Hertwig effect and photoreactivation |
description |
The optimum dose of UV irradiation to produce gynogenesis rainbow trout, Oncorhynchus mykiss with emphasis of Hertwig effect and photoreactivation (PR) was investigated. For this purpose, the sperm of rainbow trout were irradiated with UV at 2010±200 µw.cm-2intensity in different alternatives of 0, 1, 3, 5, 8, 10, 15, 20, 25, 35 and 45 minutes and allowed to fertilize normal ova; the fertilization, eyed and hatching rate were calculated to assess the performance. Using the irradiated sperm decreased the fertilization, eyed as well as hatching rates and the so-called "Hertwig effect" was observed, with the time-dependent decrease in hatching rate at 0+to 10 min of irradiation, but a better hatching rate at greater time of irradiation was observed. The highest hatching rate was observed in 20 min of UV irradiation; after that the survival rate decreased rapidly. Interestingly, irradiation even up to 45 min could not eradicate sperm fertility and a hatching rate above zero was observed at this treatment. For PR studies the semen was irradiated with UV for 5, 30 and 120 seconds and untreated semen (Os) was used as control. Irradiated semen and/or fertilized eggs by treated semen were exposed to visible light (60 W) at a distance of 30 cm for 10 min; the eyed and hatching rates were measured. The results showed that UV irradiation in as low as 5s could cause deleterious effect on semen chromatin and decrease the eyed and hatching rates (p<0.05). Illumination of semen and\or eggs with visible light resulted in PR in rainbow trout when sperm was irradiated by UV at 120s. We could conclude that the best UV irradiation time for production of gynogenesis rainbow trout was 20 min and UV irradiation, fertilization and egg hardening must be done far from visible light to eliminate the PR mechanism. |
format |
article |
topic_facet |
Biology Fisheries Gynogenesis Hertwig effect Oncorhynchus mykiss Photoreactivation Rainbow trout Ultraviolet Iran irraciation |
author |
Dorafshan, S. Kalbassi, M.R. Pourkazemi, M. Mojazi Amiri, B. |
author_facet |
Dorafshan, S. Kalbassi, M.R. Pourkazemi, M. Mojazi Amiri, B. |
author_sort |
Dorafshan, S. |
title |
Optimization of UV irradiation for production of gynogenetic rainbow trout, Oncorhynchus mykiss: emphasising Hertwig effect and photoreactivation |
title_short |
Optimization of UV irradiation for production of gynogenetic rainbow trout, Oncorhynchus mykiss: emphasising Hertwig effect and photoreactivation |
title_full |
Optimization of UV irradiation for production of gynogenetic rainbow trout, Oncorhynchus mykiss: emphasising Hertwig effect and photoreactivation |
title_fullStr |
Optimization of UV irradiation for production of gynogenetic rainbow trout, Oncorhynchus mykiss: emphasising Hertwig effect and photoreactivation |
title_full_unstemmed |
Optimization of UV irradiation for production of gynogenetic rainbow trout, Oncorhynchus mykiss: emphasising Hertwig effect and photoreactivation |
title_sort |
optimization of uv irradiation for production of gynogenetic rainbow trout, oncorhynchus mykiss: emphasising hertwig effect and photoreactivation |
publishDate |
2006 |
url |
http://hdl.handle.net/1834/37073 |
work_keys_str_mv |
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