Light and electron microscope studies of grass carp (Ctenopharyngodon idella) organs following exposure to various sublethal concentrations of diazinon
Histopathological effects of different sublethal concentrations (1, 2 and 4 mg/l) of diazinon, an organophosphorus pesticide, on gill, liver, spleen and kidney of grass carp (Ctenopharyngodon idella) were studied after 1, 7, 15, 30 and 45 days post-exposing fishes weighting 850 ± 155 g to the toxicant for 12 hours at 18-22ºC. In addition, tissues of nostril were examined for transmission electron microscopy and scanning electron microscopy. Results of light microscopic examinations of tissues showed that toxicant was causing severe histopathological effects such as congestion of blood vessels, haemorrhage, cellular infiltration, pyknosis of cells nuclei, vacuolar degeneration and general necrosis in the kidney, spleen and liver. There were also degenerative changes of interstitial tissue and detachment of tubular basement membrane in kidney observed. Hyperplasia and fusion of secondary lamellae, separation and sloughing of epithelium from the underlying basement membrane were also observed in gill sections. Transmission electron microscopy (TEM) examination of nostril revealed, damages of epithelial cells (vacuolated club cells and abnormality in cells' nuclei). Scanning electron microscopy (SEM) examination of nostril showed a reduction in excretion of amorphous proteinous materials, reduction in vesicle numbers on the cell surface and also closing of cell surface canals.
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Format: | article biblioteca |
Language: | English |
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2006
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Subjects: | Biology, Fisheries, Grass carp, Ctenopharyngodon idella, Diazinon, Histopathology, TEM, SEM, Iran, |
Online Access: | http://hdl.handle.net/1834/37071 |
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dig-aquadocs-1834-370712021-07-02T02:22:20Z Light and electron microscope studies of grass carp (Ctenopharyngodon idella) organs following exposure to various sublethal concentrations of diazinon Sharifpour, I. Pourgholam, R. Soltani, M. Hassan, M.D. Akbari, S. Nouri, A. Biology Fisheries Grass carp Ctenopharyngodon idella Diazinon Histopathology TEM SEM Iran Histopathological effects of different sublethal concentrations (1, 2 and 4 mg/l) of diazinon, an organophosphorus pesticide, on gill, liver, spleen and kidney of grass carp (Ctenopharyngodon idella) were studied after 1, 7, 15, 30 and 45 days post-exposing fishes weighting 850 ± 155 g to the toxicant for 12 hours at 18-22ºC. In addition, tissues of nostril were examined for transmission electron microscopy and scanning electron microscopy. Results of light microscopic examinations of tissues showed that toxicant was causing severe histopathological effects such as congestion of blood vessels, haemorrhage, cellular infiltration, pyknosis of cells nuclei, vacuolar degeneration and general necrosis in the kidney, spleen and liver. There were also degenerative changes of interstitial tissue and detachment of tubular basement membrane in kidney observed. Hyperplasia and fusion of secondary lamellae, separation and sloughing of epithelium from the underlying basement membrane were also observed in gill sections. Transmission electron microscopy (TEM) examination of nostril revealed, damages of epithelial cells (vacuolated club cells and abnormality in cells' nuclei). Scanning electron microscopy (SEM) examination of nostril showed a reduction in excretion of amorphous proteinous materials, reduction in vesicle numbers on the cell surface and also closing of cell surface canals. 2021-06-24T18:03:16Z 2021-06-24T18:03:16Z 2006 article TRUE 1562-2916 http://hdl.handle.net/1834/37071 en http://www.ifro.ir application/pdf application/pdf 111-136 http://aquaticcommons.org/id/eprint/22285 18721 2018-03-13 01:24:28 22285 Iranian Fisheries Science Research Institute |
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Biology Fisheries Grass carp Ctenopharyngodon idella Diazinon Histopathology TEM SEM Iran Biology Fisheries Grass carp Ctenopharyngodon idella Diazinon Histopathology TEM SEM Iran |
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Biology Fisheries Grass carp Ctenopharyngodon idella Diazinon Histopathology TEM SEM Iran Biology Fisheries Grass carp Ctenopharyngodon idella Diazinon Histopathology TEM SEM Iran Sharifpour, I. Pourgholam, R. Soltani, M. Hassan, M.D. Akbari, S. Nouri, A. Light and electron microscope studies of grass carp (Ctenopharyngodon idella) organs following exposure to various sublethal concentrations of diazinon |
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Histopathological effects of different sublethal concentrations (1, 2 and 4 mg/l) of diazinon, an organophosphorus pesticide, on gill, liver, spleen and kidney of grass carp (Ctenopharyngodon idella) were studied after 1, 7, 15, 30 and 45 days post-exposing fishes weighting 850 ± 155 g to the toxicant for 12 hours at 18-22ºC. In addition, tissues of nostril were examined for transmission electron microscopy and scanning electron microscopy. Results of light microscopic examinations of tissues showed that toxicant was causing severe histopathological effects such as congestion of blood vessels, haemorrhage, cellular infiltration, pyknosis of cells nuclei, vacuolar degeneration and general necrosis in the kidney, spleen and liver. There were also degenerative changes of interstitial tissue and detachment of tubular basement membrane in kidney observed. Hyperplasia and fusion of secondary lamellae, separation and sloughing of epithelium from the underlying basement membrane were also observed in gill sections. Transmission electron microscopy (TEM) examination of nostril revealed, damages of epithelial cells (vacuolated club cells and abnormality in cells' nuclei). Scanning electron microscopy (SEM) examination of nostril showed a reduction in excretion of amorphous proteinous materials, reduction in vesicle numbers on the cell surface and also closing of cell surface canals. |
format |
article |
topic_facet |
Biology Fisheries Grass carp Ctenopharyngodon idella Diazinon Histopathology TEM SEM Iran |
author |
Sharifpour, I. Pourgholam, R. Soltani, M. Hassan, M.D. Akbari, S. Nouri, A. |
author_facet |
Sharifpour, I. Pourgholam, R. Soltani, M. Hassan, M.D. Akbari, S. Nouri, A. |
author_sort |
Sharifpour, I. |
title |
Light and electron microscope studies of grass carp (Ctenopharyngodon idella) organs following exposure to various sublethal concentrations of diazinon |
title_short |
Light and electron microscope studies of grass carp (Ctenopharyngodon idella) organs following exposure to various sublethal concentrations of diazinon |
title_full |
Light and electron microscope studies of grass carp (Ctenopharyngodon idella) organs following exposure to various sublethal concentrations of diazinon |
title_fullStr |
Light and electron microscope studies of grass carp (Ctenopharyngodon idella) organs following exposure to various sublethal concentrations of diazinon |
title_full_unstemmed |
Light and electron microscope studies of grass carp (Ctenopharyngodon idella) organs following exposure to various sublethal concentrations of diazinon |
title_sort |
light and electron microscope studies of grass carp (ctenopharyngodon idella) organs following exposure to various sublethal concentrations of diazinon |
publishDate |
2006 |
url |
http://hdl.handle.net/1834/37071 |
work_keys_str_mv |
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