In vitro cytotoxic and anti-cancer effects of body wall for sea cucumber (Holothuria leucospilota)
In recent years efforts to find bioactive compounds from live organisms especially marine animals have been increased. In the present study, the anticancer and cytotoxic effects of sea cucumber body walls (Holothuria leucospilota) were investigated. For this purpose, sea cucumbers were collected from Larak Island at depths of 10 to 30 m and extraction process was done with methanol and diethyl ether solvent which then concentrated by rotary evaporator (40℃) following lyophilization with vacuum freeze dryer. XTT method was used to investigate anticancer and cytotoxic effects of body wall extracts. The results showed that the methanolic extract could prevent proliferation of human oral epidermoid carcinoma cells (KB) at concentrations of 100 and 500 μg/ml. The diethyl etheric extract also could prevent proliferation of KB at 500 μg/ml concentration. Overall result showed that sea cucumber body wall had a strong cytotoxic effect on normal cell line (Human embryonic kidney cell [HEK]) which can be used as potent cytotoxic material. However these extracts did not show significant therapeutic value against KB cells.
Main Authors: | , , , , |
---|---|
Format: | article biblioteca |
Language: | Persian |
Published: |
2014
|
Subjects: | Biology, Fisheries, Health, Sea cucumber, body wall, methanolic extract, diethyl etheric extract, cytotoxic, anti-cancer, Holothuria leucospilota, marine, Iran, |
Online Access: | http://hdl.handle.net/1834/36824 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-aquadocs-1834-36824 |
---|---|
record_format |
koha |
spelling |
dig-aquadocs-1834-368242021-07-06T02:27:29Z In vitro cytotoxic and anti-cancer effects of body wall for sea cucumber (Holothuria leucospilota) Bahroodi, S. Nematollahi, M.A. Aghasadeghi, M.R. Nazemi, M. Behroz, B. Biology Fisheries Health Sea cucumber body wall methanolic extract diethyl etheric extract cytotoxic anti-cancer Holothuria leucospilota marine Iran In recent years efforts to find bioactive compounds from live organisms especially marine animals have been increased. In the present study, the anticancer and cytotoxic effects of sea cucumber body walls (Holothuria leucospilota) were investigated. For this purpose, sea cucumbers were collected from Larak Island at depths of 10 to 30 m and extraction process was done with methanol and diethyl ether solvent which then concentrated by rotary evaporator (40℃) following lyophilization with vacuum freeze dryer. XTT method was used to investigate anticancer and cytotoxic effects of body wall extracts. The results showed that the methanolic extract could prevent proliferation of human oral epidermoid carcinoma cells (KB) at concentrations of 100 and 500 μg/ml. The diethyl etheric extract also could prevent proliferation of KB at 500 μg/ml concentration. Overall result showed that sea cucumber body wall had a strong cytotoxic effect on normal cell line (Human embryonic kidney cell [HEK]) which can be used as potent cytotoxic material. However these extracts did not show significant therapeutic value against KB cells. 2021-06-24T18:01:13Z 2021-06-24T18:01:13Z 2014 article TRUE 1026-1354 http://hdl.handle.net/1834/36824 fa http://isfj.areo.ir/?lang=en application/pdf application/pdf 11-20 http://aquaticcommons.org/id/eprint/22011 18721 2018-01-22 11:09:49 22011 Iranian Fisheries Science Research Institute |
institution |
UNESCO |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-aquadocs |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Repositorio AQUADOCS |
language |
Persian |
topic |
Biology Fisheries Health Sea cucumber body wall methanolic extract diethyl etheric extract cytotoxic anti-cancer Holothuria leucospilota marine Iran Biology Fisheries Health Sea cucumber body wall methanolic extract diethyl etheric extract cytotoxic anti-cancer Holothuria leucospilota marine Iran |
spellingShingle |
Biology Fisheries Health Sea cucumber body wall methanolic extract diethyl etheric extract cytotoxic anti-cancer Holothuria leucospilota marine Iran Biology Fisheries Health Sea cucumber body wall methanolic extract diethyl etheric extract cytotoxic anti-cancer Holothuria leucospilota marine Iran Bahroodi, S. Nematollahi, M.A. Aghasadeghi, M.R. Nazemi, M. Behroz, B. In vitro cytotoxic and anti-cancer effects of body wall for sea cucumber (Holothuria leucospilota) |
description |
In recent years efforts to find bioactive compounds from live organisms especially marine animals have been increased. In the present study, the anticancer and cytotoxic effects of sea cucumber body walls (Holothuria leucospilota) were investigated. For this purpose, sea cucumbers were collected from Larak Island at depths of 10 to 30 m and extraction process was done with methanol and diethyl ether solvent which then concentrated by rotary evaporator (40℃) following lyophilization with vacuum freeze dryer. XTT method was used to investigate anticancer and cytotoxic effects of body wall extracts. The results showed that the methanolic extract could prevent proliferation of human oral epidermoid carcinoma cells (KB) at concentrations of 100 and 500 μg/ml. The diethyl etheric extract also could prevent proliferation of KB at 500 μg/ml concentration. Overall result showed that sea cucumber body wall had a strong cytotoxic effect on normal cell line (Human embryonic kidney cell [HEK]) which can be used as potent cytotoxic material. However these extracts did not show significant therapeutic value against KB cells. |
format |
article |
topic_facet |
Biology Fisheries Health Sea cucumber body wall methanolic extract diethyl etheric extract cytotoxic anti-cancer Holothuria leucospilota marine Iran |
author |
Bahroodi, S. Nematollahi, M.A. Aghasadeghi, M.R. Nazemi, M. Behroz, B. |
author_facet |
Bahroodi, S. Nematollahi, M.A. Aghasadeghi, M.R. Nazemi, M. Behroz, B. |
author_sort |
Bahroodi, S. |
title |
In vitro cytotoxic and anti-cancer effects of body wall for sea cucumber (Holothuria leucospilota) |
title_short |
In vitro cytotoxic and anti-cancer effects of body wall for sea cucumber (Holothuria leucospilota) |
title_full |
In vitro cytotoxic and anti-cancer effects of body wall for sea cucumber (Holothuria leucospilota) |
title_fullStr |
In vitro cytotoxic and anti-cancer effects of body wall for sea cucumber (Holothuria leucospilota) |
title_full_unstemmed |
In vitro cytotoxic and anti-cancer effects of body wall for sea cucumber (Holothuria leucospilota) |
title_sort |
in vitro cytotoxic and anti-cancer effects of body wall for sea cucumber (holothuria leucospilota) |
publishDate |
2014 |
url |
http://hdl.handle.net/1834/36824 |
work_keys_str_mv |
AT bahroodis invitrocytotoxicandanticancereffectsofbodywallforseacucumberholothurialeucospilota AT nematollahima invitrocytotoxicandanticancereffectsofbodywallforseacucumberholothurialeucospilota AT aghasadeghimr invitrocytotoxicandanticancereffectsofbodywallforseacucumberholothurialeucospilota AT nazemim invitrocytotoxicandanticancereffectsofbodywallforseacucumberholothurialeucospilota AT behrozb invitrocytotoxicandanticancereffectsofbodywallforseacucumberholothurialeucospilota |
_version_ |
1756079542804414464 |