In-vitro effects of biochemical factors on trypsin activity from intestine and pyloric caeca of common kilka (Clupeonella cultriventris caspia) for inhibition of belly bursting
The in-vitro effects of biochemical factors on trypsin activity from intestine and pyloric caeca of common kilka (Clupeonella cultriventris caspia) was evaluated. Trypsin is known to cause belly bursting in common kilka. The assessments showed that in a range of 4-70°C, trypsin from pyloric caeca and intestine of common kilka had the maximum activity and the satiability at 60 and 55°C, respectively. The pH assessments indicated that maximum activity and stability for trypsin were at 8.5 at pH range of 4-11. The effects of metal ions on trypsin activity revealed that CaCl2, MgCl2 and MnCl2 increased trypsin activity while CuCl2 ،ZnCl2 and Al2(SO4)3 decreased its activity. The effect of inhibitors on trypsin activity also showed that SBTI and TLCK (specific inhibitors for trypsin) significantly inhibited trypsin activity. This study suggests that belly bursting in common kilka can be prevented by trypsin inactivation through application of low temperature (4°C), acidic pH, metals of CuCl2, ZnCl2 and Al2(SO4)3 and inhibitors of SBTI and TLCK.
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Format: | article biblioteca |
Language: | Persian |
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2012
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Subjects: | Biology, Chemistry, Feeding, Digestion system, Ventral keel, Inhibitor components, iran, Clupeonella cultriventris caspia, In-vitro, kilka, |
Online Access: | http://hdl.handle.net/1834/36680 |
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dig-aquadocs-1834-366802021-07-03T04:09:11Z In-vitro effects of biochemical factors on trypsin activity from intestine and pyloric caeca of common kilka (Clupeonella cultriventris caspia) for inhibition of belly bursting Zamani, A. Rezaei, M. Madani, R. Biology Chemistry Feeding Digestion system Ventral keel Inhibitor components iran Clupeonella cultriventris caspia In-vitro kilka The in-vitro effects of biochemical factors on trypsin activity from intestine and pyloric caeca of common kilka (Clupeonella cultriventris caspia) was evaluated. Trypsin is known to cause belly bursting in common kilka. The assessments showed that in a range of 4-70°C, trypsin from pyloric caeca and intestine of common kilka had the maximum activity and the satiability at 60 and 55°C, respectively. The pH assessments indicated that maximum activity and stability for trypsin were at 8.5 at pH range of 4-11. The effects of metal ions on trypsin activity revealed that CaCl2, MgCl2 and MnCl2 increased trypsin activity while CuCl2 ،ZnCl2 and Al2(SO4)3 decreased its activity. The effect of inhibitors on trypsin activity also showed that SBTI and TLCK (specific inhibitors for trypsin) significantly inhibited trypsin activity. This study suggests that belly bursting in common kilka can be prevented by trypsin inactivation through application of low temperature (4°C), acidic pH, metals of CuCl2, ZnCl2 and Al2(SO4)3 and inhibitors of SBTI and TLCK. 2021-06-24T18:00:18Z 2021-06-24T18:00:18Z 2012 article FALSE 1026-1354 http://hdl.handle.net/1834/36680 fa http://isfj.areo.ir/?lang=en application/pdf application/pdf 53-62 http://aquaticcommons.org/id/eprint/21865 18721 2018-01-15 06:43:20 21865 Iranian Fisheries Science Research Institute |
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Biology Chemistry Feeding Digestion system Ventral keel Inhibitor components iran Clupeonella cultriventris caspia In-vitro kilka Biology Chemistry Feeding Digestion system Ventral keel Inhibitor components iran Clupeonella cultriventris caspia In-vitro kilka |
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Biology Chemistry Feeding Digestion system Ventral keel Inhibitor components iran Clupeonella cultriventris caspia In-vitro kilka Biology Chemistry Feeding Digestion system Ventral keel Inhibitor components iran Clupeonella cultriventris caspia In-vitro kilka Zamani, A. Rezaei, M. Madani, R. In-vitro effects of biochemical factors on trypsin activity from intestine and pyloric caeca of common kilka (Clupeonella cultriventris caspia) for inhibition of belly bursting |
description |
The in-vitro effects of biochemical factors on trypsin activity from intestine and pyloric caeca of common kilka (Clupeonella cultriventris caspia) was evaluated. Trypsin is known to cause belly bursting in common kilka. The assessments showed that in a range of 4-70°C, trypsin from pyloric caeca and intestine of common kilka had the maximum activity and the satiability at 60 and 55°C, respectively. The pH assessments indicated that maximum activity and stability for trypsin were at 8.5 at pH range of 4-11. The effects of metal ions on trypsin activity revealed that CaCl2, MgCl2 and MnCl2 increased trypsin activity while CuCl2 ،ZnCl2 and Al2(SO4)3 decreased its activity. The effect of inhibitors on trypsin activity also showed that SBTI and TLCK (specific inhibitors for trypsin) significantly inhibited trypsin activity. This study suggests that belly bursting in common kilka can be prevented by trypsin inactivation through application of low temperature (4°C), acidic pH, metals of CuCl2, ZnCl2 and Al2(SO4)3 and inhibitors of SBTI and TLCK. |
format |
article |
topic_facet |
Biology Chemistry Feeding Digestion system Ventral keel Inhibitor components iran Clupeonella cultriventris caspia In-vitro kilka |
author |
Zamani, A. Rezaei, M. Madani, R. |
author_facet |
Zamani, A. Rezaei, M. Madani, R. |
author_sort |
Zamani, A. |
title |
In-vitro effects of biochemical factors on trypsin activity from intestine and pyloric caeca of common kilka (Clupeonella cultriventris caspia) for inhibition of belly bursting |
title_short |
In-vitro effects of biochemical factors on trypsin activity from intestine and pyloric caeca of common kilka (Clupeonella cultriventris caspia) for inhibition of belly bursting |
title_full |
In-vitro effects of biochemical factors on trypsin activity from intestine and pyloric caeca of common kilka (Clupeonella cultriventris caspia) for inhibition of belly bursting |
title_fullStr |
In-vitro effects of biochemical factors on trypsin activity from intestine and pyloric caeca of common kilka (Clupeonella cultriventris caspia) for inhibition of belly bursting |
title_full_unstemmed |
In-vitro effects of biochemical factors on trypsin activity from intestine and pyloric caeca of common kilka (Clupeonella cultriventris caspia) for inhibition of belly bursting |
title_sort |
in-vitro effects of biochemical factors on trypsin activity from intestine and pyloric caeca of common kilka (clupeonella cultriventris caspia) for inhibition of belly bursting |
publishDate |
2012 |
url |
http://hdl.handle.net/1834/36680 |
work_keys_str_mv |
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