Genetic analysis of pike-perch, Sander lucioperca L., populations revealed by microsatellite DNA markers in Iran
This study was conducted in order to investigate genetic diversity and population structure of pike perch in the Northern part of Iran. For this purpose, 207 adult pike-perches from four regions of the Caspian Sea watershed (Talesh Coasts, Anzali Wetland, Chaboksar Coasts and Aras Dam) were collected. DNA was extracted and by using 15 pairs of microsatellite primers, Polymerase Chain Reaction (PCR) was conducted. DNA bands were analyzed using Biocapt and GenAlex 6 software package. Out of 15 microsatellite primers, 11 loci were produced, of those, 6 loci were polymorphic and 5 were monomorphic. Analysis revealed that the average number of alleles per locus and observed heterozygosities were not statistically significant (P>0.05) for all four populations. Data indicated an appreciable genetic differentiation, in spite of a low genetic variation, and agreed with the low level of genetic polymorphism already observed for this species in Iran. Deviation from Hardy-Weinberg equilibrium was obvious in most cases, mostly due to the deficiency of heterozygosities. The highest genetic distance was between Anzali Wetland and Aras Dam populations. This investigation represents the first approach to the knowledge of the genetic variability of Iranian populations using microsatellite markers, and reported results could be of interest for future management and conservation programs of this species in Iran.
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Language: | English |
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2014
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Subjects: | Biology, Fisheries, genetic, Sander lucioperca, populations, microsatellite, DNA, markers, iran, polymorphism, |
Online Access: | http://hdl.handle.net/1834/36559 |
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dig-aquadocs-1834-365592021-06-30T01:58:12Z Genetic analysis of pike-perch, Sander lucioperca L., populations revealed by microsatellite DNA markers in Iran Gharibkhani, M. Pourkazemi, M. Rezvani Gilkolai, S. Tatina, M. Azizzadeh, L. Biology Fisheries genetic Sander lucioperca populations microsatellite DNA markers iran polymorphism This study was conducted in order to investigate genetic diversity and population structure of pike perch in the Northern part of Iran. For this purpose, 207 adult pike-perches from four regions of the Caspian Sea watershed (Talesh Coasts, Anzali Wetland, Chaboksar Coasts and Aras Dam) were collected. DNA was extracted and by using 15 pairs of microsatellite primers, Polymerase Chain Reaction (PCR) was conducted. DNA bands were analyzed using Biocapt and GenAlex 6 software package. Out of 15 microsatellite primers, 11 loci were produced, of those, 6 loci were polymorphic and 5 were monomorphic. Analysis revealed that the average number of alleles per locus and observed heterozygosities were not statistically significant (P>0.05) for all four populations. Data indicated an appreciable genetic differentiation, in spite of a low genetic variation, and agreed with the low level of genetic polymorphism already observed for this species in Iran. Deviation from Hardy-Weinberg equilibrium was obvious in most cases, mostly due to the deficiency of heterozygosities. The highest genetic distance was between Anzali Wetland and Aras Dam populations. This investigation represents the first approach to the knowledge of the genetic variability of Iranian populations using microsatellite markers, and reported results could be of interest for future management and conservation programs of this species in Iran. 2021-06-24T17:59:26Z 2021-06-24T17:59:26Z 2014 article TRUE 1735-3033 http://hdl.handle.net/1834/36559 en http://cjes.guilan.ac.ir/ application/pdf application/pdf 99-108 http://aquaticcommons.org/id/eprint/21736 18721 2017-11-28 09:02:29 21736 University of Guilan, Faculty of Natural Resources, Iran |
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Biology Fisheries genetic Sander lucioperca populations microsatellite DNA markers iran polymorphism Biology Fisheries genetic Sander lucioperca populations microsatellite DNA markers iran polymorphism |
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Biology Fisheries genetic Sander lucioperca populations microsatellite DNA markers iran polymorphism Biology Fisheries genetic Sander lucioperca populations microsatellite DNA markers iran polymorphism Gharibkhani, M. Pourkazemi, M. Rezvani Gilkolai, S. Tatina, M. Azizzadeh, L. Genetic analysis of pike-perch, Sander lucioperca L., populations revealed by microsatellite DNA markers in Iran |
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This study was conducted in order to investigate genetic diversity and population structure of pike perch in the Northern part of Iran. For this purpose, 207 adult pike-perches from four regions of the Caspian Sea watershed (Talesh Coasts, Anzali Wetland, Chaboksar Coasts and Aras Dam) were collected. DNA was extracted and by using 15 pairs of microsatellite primers, Polymerase Chain Reaction (PCR) was conducted. DNA bands were analyzed using Biocapt and GenAlex 6 software package. Out of 15 microsatellite primers, 11 loci were produced, of those, 6 loci were polymorphic and 5 were monomorphic. Analysis revealed that the average number of alleles per locus and observed heterozygosities were not statistically significant (P>0.05) for all four populations. Data indicated an appreciable genetic differentiation, in spite of a low genetic variation, and agreed with the low level of genetic polymorphism already observed for this species in Iran. Deviation from Hardy-Weinberg equilibrium was obvious in most cases, mostly due to the deficiency of heterozygosities. The highest genetic distance was between Anzali Wetland and Aras Dam populations. This investigation represents the first approach to the knowledge of the genetic variability of Iranian populations using microsatellite markers, and reported results could be of interest for future management and conservation programs of this species in Iran. |
format |
article |
topic_facet |
Biology Fisheries genetic Sander lucioperca populations microsatellite DNA markers iran polymorphism |
author |
Gharibkhani, M. Pourkazemi, M. Rezvani Gilkolai, S. Tatina, M. Azizzadeh, L. |
author_facet |
Gharibkhani, M. Pourkazemi, M. Rezvani Gilkolai, S. Tatina, M. Azizzadeh, L. |
author_sort |
Gharibkhani, M. |
title |
Genetic analysis of pike-perch, Sander lucioperca L., populations revealed by microsatellite DNA markers in Iran |
title_short |
Genetic analysis of pike-perch, Sander lucioperca L., populations revealed by microsatellite DNA markers in Iran |
title_full |
Genetic analysis of pike-perch, Sander lucioperca L., populations revealed by microsatellite DNA markers in Iran |
title_fullStr |
Genetic analysis of pike-perch, Sander lucioperca L., populations revealed by microsatellite DNA markers in Iran |
title_full_unstemmed |
Genetic analysis of pike-perch, Sander lucioperca L., populations revealed by microsatellite DNA markers in Iran |
title_sort |
genetic analysis of pike-perch, sander lucioperca l., populations revealed by microsatellite dna markers in iran |
publishDate |
2014 |
url |
http://hdl.handle.net/1834/36559 |
work_keys_str_mv |
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_version_ |
1756079507432800256 |