Chronotropic and inotropic effects of adenosine and AMP on the isolated systemic heart of Octopus vulgaris
The effect of adenosine on the function of the heart in Octopus vulgaris was studied using an isolated heart preparation. Bolus injections of adenosine or AMP (adenosine precursor) induced both positive chronotropic and inotropic effects. The maximum inotropic effect preceded the maximum chronotropic effect. The impermeable adenosine analogue 2-chloroadenosine elicited a similar effect, while the adenosine uptake blocker dipyridamole did not affect the adenosine response. These results suggest that adenosine acted extracellularly. The concentration-response curves of adenosine and AMP were also determined, by evaluating the effects on ventricular and coronary function. Under these conditions, the potent chronotropic effect elicited by both substances apparently masked or compensated for the inotropic effect, owing to the negative force-frequency relationship known to occur in the octopus heart. The AMP displayed a lower threshold than adenosine, suggesting an higher affinity for the purinergic receptors involved or a strict association between 5'-nucleotidase and the adenosine receptor on the plasma membrane.
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Format: | article biblioteca |
Language: | English |
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1997
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Subjects: | Biology, adenosine, AMP, Octopus vulgaris, systemic heart, coronary circulation, |
Online Access: | http://hdl.handle.net/1834/34423 |
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dig-aquadocs-1834-344232021-07-13T02:55:05Z Chronotropic and inotropic effects of adenosine and AMP on the isolated systemic heart of Octopus vulgaris Agnisola, C. Conte, M. Biology adenosine AMP Octopus vulgaris systemic heart coronary circulation The effect of adenosine on the function of the heart in Octopus vulgaris was studied using an isolated heart preparation. Bolus injections of adenosine or AMP (adenosine precursor) induced both positive chronotropic and inotropic effects. The maximum inotropic effect preceded the maximum chronotropic effect. The impermeable adenosine analogue 2-chloroadenosine elicited a similar effect, while the adenosine uptake blocker dipyridamole did not affect the adenosine response. These results suggest that adenosine acted extracellularly. The concentration-response curves of adenosine and AMP were also determined, by evaluating the effects on ventricular and coronary function. Under these conditions, the potent chronotropic effect elicited by both substances apparently masked or compensated for the inotropic effect, owing to the negative force-frequency relationship known to occur in the octopus heart. The AMP displayed a lower threshold than adenosine, suggesting an higher affinity for the purinergic receptors involved or a strict association between 5'-nucleotidase and the adenosine receptor on the plasma membrane. 2021-06-24T17:38:22Z 2021-06-24T17:38:22Z 1997 article 1019-8415 http://hdl.handle.net/1834/34423 en application/pdf application/pdf 39-48 http://aquaticcommons.org/id/eprint/19165 12051 2015-12-18 19:39:25 19165 University of Karachi. Marine Reference Collection and Resource Centre |
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Biology adenosine AMP Octopus vulgaris systemic heart coronary circulation Biology adenosine AMP Octopus vulgaris systemic heart coronary circulation |
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Biology adenosine AMP Octopus vulgaris systemic heart coronary circulation Biology adenosine AMP Octopus vulgaris systemic heart coronary circulation Agnisola, C. Conte, M. Chronotropic and inotropic effects of adenosine and AMP on the isolated systemic heart of Octopus vulgaris |
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The effect of adenosine on the function of the heart in Octopus vulgaris was studied using an isolated heart preparation. Bolus injections of adenosine or AMP (adenosine precursor) induced both positive chronotropic and inotropic effects. The maximum inotropic effect preceded the maximum chronotropic effect. The impermeable adenosine analogue 2-chloroadenosine elicited a similar effect, while the adenosine uptake blocker dipyridamole did not affect the adenosine response. These results suggest that adenosine acted extracellularly. The concentration-response curves of adenosine and AMP were also determined, by evaluating the effects on ventricular and coronary function. Under these conditions, the potent chronotropic effect elicited by both substances apparently masked or compensated for the inotropic effect, owing to the negative force-frequency relationship known to occur in the octopus heart. The AMP displayed a lower threshold than adenosine, suggesting an higher affinity for the purinergic receptors involved or a strict association between 5'-nucleotidase and the adenosine receptor on the plasma membrane. |
format |
article |
topic_facet |
Biology adenosine AMP Octopus vulgaris systemic heart coronary circulation |
author |
Agnisola, C. Conte, M. |
author_facet |
Agnisola, C. Conte, M. |
author_sort |
Agnisola, C. |
title |
Chronotropic and inotropic effects of adenosine and AMP on the isolated systemic heart of Octopus vulgaris |
title_short |
Chronotropic and inotropic effects of adenosine and AMP on the isolated systemic heart of Octopus vulgaris |
title_full |
Chronotropic and inotropic effects of adenosine and AMP on the isolated systemic heart of Octopus vulgaris |
title_fullStr |
Chronotropic and inotropic effects of adenosine and AMP on the isolated systemic heart of Octopus vulgaris |
title_full_unstemmed |
Chronotropic and inotropic effects of adenosine and AMP on the isolated systemic heart of Octopus vulgaris |
title_sort |
chronotropic and inotropic effects of adenosine and amp on the isolated systemic heart of octopus vulgaris |
publishDate |
1997 |
url |
http://hdl.handle.net/1834/34423 |
work_keys_str_mv |
AT agnisolac chronotropicandinotropiceffectsofadenosineandampontheisolatedsystemicheartofoctopusvulgaris AT contem chronotropicandinotropiceffectsofadenosineandampontheisolatedsystemicheartofoctopusvulgaris |
_version_ |
1756079223310647296 |