Cultural methods of detection for microorganisms: recent advances and successes
Most microbiological methods require culture to allow organisms to recover or to selectively increase, and target organisms are identified by growth on specific agar media. Many cultural methods take several days to complete and even then the results require confirmation. Alternative techniques include the use of chromogenic and fluorogenic substances to identify bacteria as they are growing, selective capture using antibodies after short periods of growth, molecular techniques, and direct staining with or without flow cytometry for enumeration and identification. Future microbiologists may not use culture but depend on the use of specific probes and sophisticated detection systems.
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| Format: | book_section biblioteca |
| Language: | English |
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Freshwater Biological Association
1997
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| Subjects: | Engineering, Limnology, Pollution, Water quality, Microorganisms, Methodology, Detection, Bacteria, Cultured organisms, Enzymes, Immunofluorescence, Flow cytometry, Microbiological culture, |
| Online Access: | http://hdl.handle.net/1834/22824 |
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dig-aquadocs-1834-228242021-07-09T03:20:15Z Cultural methods of detection for microorganisms: recent advances and successes The microbiological quality of water Watkins , J. Jian, X. Sutcliffe, D.W. Engineering Limnology Pollution Water quality Microorganisms Methodology Detection Bacteria Cultured organisms Enzymes Immunofluorescence Flow cytometry Microbiological culture Most microbiological methods require culture to allow organisms to recover or to selectively increase, and target organisms are identified by growth on specific agar media. Many cultural methods take several days to complete and even then the results require confirmation. Alternative techniques include the use of chromogenic and fluorogenic substances to identify bacteria as they are growing, selective capture using antibodies after short periods of growth, molecular techniques, and direct staining with or without flow cytometry for enumeration and identification. Future microbiologists may not use culture but depend on the use of specific probes and sophisticated detection systems. 2021-06-24T16:04:56Z 2021-06-24T16:04:56Z 1997 book_section FALSE 0-900386-57-6 http://hdl.handle.net/1834/22824 en FBA Special Publications http://www.fba.org.uk/spec5 application/pdf application/pdf 19-27 Freshwater Biological Association Ambleside, UK http://aquaticcommons.org/id/eprint/5317 1256 2011-09-29 15:05:35 5317 Freshwater Biological Association |
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biblioteca |
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| language |
English |
| topic |
Engineering Limnology Pollution Water quality Microorganisms Methodology Detection Bacteria Cultured organisms Enzymes Immunofluorescence Flow cytometry Microbiological culture Engineering Limnology Pollution Water quality Microorganisms Methodology Detection Bacteria Cultured organisms Enzymes Immunofluorescence Flow cytometry Microbiological culture |
| spellingShingle |
Engineering Limnology Pollution Water quality Microorganisms Methodology Detection Bacteria Cultured organisms Enzymes Immunofluorescence Flow cytometry Microbiological culture Engineering Limnology Pollution Water quality Microorganisms Methodology Detection Bacteria Cultured organisms Enzymes Immunofluorescence Flow cytometry Microbiological culture Watkins , J. Jian, X. Cultural methods of detection for microorganisms: recent advances and successes |
| description |
Most microbiological methods require culture to allow organisms to recover or to selectively increase, and target organisms are identified by growth on specific agar media. Many cultural methods take several days to complete and even then the results require confirmation. Alternative techniques include the use of chromogenic and fluorogenic substances to identify bacteria as they are growing, selective capture using antibodies after short periods of growth, molecular techniques, and direct staining with or without flow cytometry for enumeration and identification. Future microbiologists may not use culture but depend on the use of specific probes and sophisticated detection systems. |
| author2 |
Sutcliffe, D.W. |
| author_facet |
Sutcliffe, D.W. Watkins , J. Jian, X. |
| format |
book_section |
| topic_facet |
Engineering Limnology Pollution Water quality Microorganisms Methodology Detection Bacteria Cultured organisms Enzymes Immunofluorescence Flow cytometry Microbiological culture |
| author |
Watkins , J. Jian, X. |
| author_sort |
Watkins , J. |
| title |
Cultural methods of detection for microorganisms: recent advances and successes |
| title_short |
Cultural methods of detection for microorganisms: recent advances and successes |
| title_full |
Cultural methods of detection for microorganisms: recent advances and successes |
| title_fullStr |
Cultural methods of detection for microorganisms: recent advances and successes |
| title_full_unstemmed |
Cultural methods of detection for microorganisms: recent advances and successes |
| title_sort |
cultural methods of detection for microorganisms: recent advances and successes |
| publisher |
Freshwater Biological Association |
| publishDate |
1997 |
| url |
http://hdl.handle.net/1834/22824 |
| work_keys_str_mv |
AT watkinsj culturalmethodsofdetectionformicroorganismsrecentadvancesandsuccesses AT jianx culturalmethodsofdetectionformicroorganismsrecentadvancesandsuccesses AT watkinsj themicrobiologicalqualityofwater AT jianx themicrobiologicalqualityofwater |
| _version_ |
1756077682134614016 |