Preliminary study on the natural extenders for artificial breeding of African catfish Clarias gariepinus (Burchell, 1822)

The objective of the present study was to determine the most suitable extender and theirrespective dilution ratios for African catfish sperm for artificial induced breeding and cryopreservationpurposes. Three natural extenders were tested i.e. coconut water, sugarcane water and soybeansolutions, at three different levels of sperm to extender dilutions of 1:20, 1:30 and 1:40. While Ringersolution was used as a control Diluted sperm were fertilized with ready isolated eggs to assess thefertility and hatching rate at 0, 6 and 12 hour intervals. The results showed that the eggs hatchedapproximately 19 to 27 hours after fertilization. In general, the fertilization and hatching rates decreasedwith increasing dilution ratio. With respect to natural extenders, the coconut water showed the highestfertility and hatching rates at 1:20 dilution ratio. Therefore, coconut water at 1:20 dilution ratio was theoptimal condition for African catfish spermatozoa among the natural extenders investigated.

Bibliographic Details

Main Authors:	Muchlisin, Z.A, Siti-Azizah, M.N.
Format:	article biblioteca
Language:	English
Published:	2010
Subjects:	Fisheries, Aquaculture,
Online Access:	http://hdl.handle.net/1834/21357
Tags:	Add Tag No Tags, Be the first to tag this record!