Monitoring hydrilla using two RAPD procedures and the Nonindigenous Aquatic Species database
Hydrilla (Hydrilla verticillata(L.f.) Royle), an invasiveaquatic weed, continues to spread to new regions in the UnitedStates. Two biotypes, one a female dioecious and the othermonoecious have been identified. Management of thespread of hydrilla requires understanding the mechanisms ofintroduction and transport, an ability to map and make availableinformation on distribution, and tools to distinguish theknown U.S. biotypes as well as potential new introductions.Review of the literature and discussions with aquatic scientistsand resource managers point to the aquarium and watergarden plant trades as the primary past mechanism for theregional dispersal of hydrilla while local dispersal is primarilycarried out by other mechanisms such as boat traffic, intentionalintroductions, and waterfowl. The NonindigenousAquatic Species (NAS) database is presented as a tool for assembling,geo-referencing, and making available informationon the distribution of hydrilla. A map of the currentrange of dioecious and monoecious hydrilla by drainage ispresented. Four hydrilla samples, taken from three discrete,non-contiguous regions (Pennsylvania, Connecticut, andWashington State) were examined using two RAPD assays.The first, generated using primer Operon G17, and capableof distinguishing the dioecious and monoecious U.S. biotypes,indicated all four samples were of the monoecious biotype.Results of the second assay using the Stoffel fragmentand 5 primers, produced 111 markers, indicated that thesesamples do not represent new foreign introductions. The differencesin the monoecious and dioecious growth habits andmanagement are discussed.
| Main Authors: | , , |
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| Format: | article biblioteca |
| Language: | English |
| Published: |
2000
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| Subjects: | Management, Biology, Limnology, Randomly amplified polymorphic DNA, Hydrilla verticillata, monoecious, dioecious, spread, dispersal, Washington, Connecticut, Pennsylvania, |
| Online Access: | http://hdl.handle.net/1834/19657 |
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