Inflammatory cytokines responses of common carp, Cyprinus carpio, leucocytes in vitro treated by immunostimulants
Cytokines are important regulators of the immune system, and identifying fish cytokines has potential applications for the development of vaccines and/or immunostimulants application in fisheries. In order to understand the immune-related genes triggered by immunostimulants derived from pathogens, we investigated the effects of agonists (lipopolysaccharide (LPS), Poly I:C, and imiquimod) of three Toll-like receptor (TLR)—TLR4, TLR3, and TLR7, respectively—on the expression level of 10 cytokine genes—interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), CXC-chemokine, interleukin-10 (IL-10), type1 interferon (1INF), IFN-γ1, IFN-γ2, Mx protein, and interleukin-12 (IL-12p35 and IL-12 p40)—in the head kidney leukocytes of common carp (Cyprinus carpio L.). All cytokine genes tested, except for type-1 IFN, were significantly up-regulated after LPS treatment. Poly I:C and imiquimod treatment resulted in striking up-regulation of most of the genes examined, particularly for the interferon genes as compared with the control groups. These results clearly demonstrate that TLRs agonists elicit the signaling pathways for cytokines production and initiation of innate immune responses in common carp. The development of strategies to control the pathogen load and of immune-prophylactic measures must be addressed further to realize the economic potential of fish production.
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Format: | Journal Contribution biblioteca |
Language: | English |
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2019
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Subjects: | Cyprinus carpio, Fish, Cytokines, Immune system, Vaccine, Pathogens, |
Online Access: | http://hdl.handle.net/1834/16286 |
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dig-aquadocs-1834-162862021-05-19T06:43:31Z Inflammatory cytokines responses of common carp, Cyprinus carpio, leucocytes in vitro treated by immunostimulants Tanekhy, M. Sakai, M. Cyprinus carpio Fish Cytokines Immune system Vaccine Pathogens Cytokines are important regulators of the immune system, and identifying fish cytokines has potential applications for the development of vaccines and/or immunostimulants application in fisheries. In order to understand the immune-related genes triggered by immunostimulants derived from pathogens, we investigated the effects of agonists (lipopolysaccharide (LPS), Poly I:C, and imiquimod) of three Toll-like receptor (TLR)—TLR4, TLR3, and TLR7, respectively—on the expression level of 10 cytokine genes—interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), CXC-chemokine, interleukin-10 (IL-10), type1 interferon (1INF), IFN-γ1, IFN-γ2, Mx protein, and interleukin-12 (IL-12p35 and IL-12 p40)—in the head kidney leukocytes of common carp (Cyprinus carpio L.). All cytokine genes tested, except for type-1 IFN, were significantly up-regulated after LPS treatment. Poly I:C and imiquimod treatment resulted in striking up-regulation of most of the genes examined, particularly for the interferon genes as compared with the control groups. These results clearly demonstrate that TLRs agonists elicit the signaling pathways for cytokines production and initiation of innate immune responses in common carp. The development of strategies to control the pathogen load and of immune-prophylactic measures must be addressed further to realize the economic potential of fish production. Published 2020-02-16T07:49:51Z 2020-02-16T07:49:51Z 2019 Journal Contribution Refereed 1562-2916 http://hdl.handle.net/1834/16286 en http://jifro.ir pp.847-861 Egypt |
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Cyprinus carpio Fish Cytokines Immune system Vaccine Pathogens Cyprinus carpio Fish Cytokines Immune system Vaccine Pathogens |
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Cyprinus carpio Fish Cytokines Immune system Vaccine Pathogens Cyprinus carpio Fish Cytokines Immune system Vaccine Pathogens Tanekhy, M. Sakai, M. Inflammatory cytokines responses of common carp, Cyprinus carpio, leucocytes in vitro treated by immunostimulants |
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Cytokines are important regulators of the immune system, and identifying fish cytokines has potential applications for the development of vaccines and/or immunostimulants application in fisheries. In order to understand the immune-related genes triggered by immunostimulants derived from pathogens, we investigated the effects of agonists (lipopolysaccharide (LPS), Poly I:C, and imiquimod) of three Toll-like receptor (TLR)—TLR4, TLR3, and TLR7, respectively—on the expression level of 10 cytokine genes—interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), CXC-chemokine, interleukin-10 (IL-10), type1 interferon (1INF), IFN-γ1, IFN-γ2, Mx protein, and interleukin-12 (IL-12p35 and IL-12 p40)—in the head kidney leukocytes of common carp (Cyprinus carpio L.). All cytokine genes tested, except for type-1 IFN, were significantly up-regulated after LPS treatment. Poly I:C and imiquimod treatment resulted in striking up-regulation of most of the genes examined, particularly for the interferon genes as compared with the control groups. These results clearly demonstrate that TLRs agonists elicit the signaling pathways for cytokines production and initiation of innate immune responses in common carp. The development of strategies to control the pathogen load and of immune-prophylactic measures must be addressed further to realize the economic potential of fish production. |
format |
Journal Contribution |
topic_facet |
Cyprinus carpio Fish Cytokines Immune system Vaccine Pathogens |
author |
Tanekhy, M. Sakai, M. |
author_facet |
Tanekhy, M. Sakai, M. |
author_sort |
Tanekhy, M. |
title |
Inflammatory cytokines responses of common carp, Cyprinus carpio, leucocytes in vitro treated by immunostimulants |
title_short |
Inflammatory cytokines responses of common carp, Cyprinus carpio, leucocytes in vitro treated by immunostimulants |
title_full |
Inflammatory cytokines responses of common carp, Cyprinus carpio, leucocytes in vitro treated by immunostimulants |
title_fullStr |
Inflammatory cytokines responses of common carp, Cyprinus carpio, leucocytes in vitro treated by immunostimulants |
title_full_unstemmed |
Inflammatory cytokines responses of common carp, Cyprinus carpio, leucocytes in vitro treated by immunostimulants |
title_sort |
inflammatory cytokines responses of common carp, cyprinus carpio, leucocytes in vitro treated by immunostimulants |
publishDate |
2019 |
url |
http://hdl.handle.net/1834/16286 |
work_keys_str_mv |
AT tanekhym inflammatorycytokinesresponsesofcommoncarpcyprinuscarpioleucocytesinvitrotreatedbyimmunostimulants AT sakaim inflammatorycytokinesresponsesofcommoncarpcyprinuscarpioleucocytesinvitrotreatedbyimmunostimulants |
_version_ |
1756076216043962368 |