Characteristic of bacterial communities associated with mollusc biodeposits

Quantitative and qualitative composition of the bacterioflora of biosediments produced by mussels and oysters were studied in the southern Kerch Strait, area of potential experimental and commercial culture of molluscs. Sampling was made from May to October 1993. A total of 45 various groups of microorganisms were determined and 50 pure bacterial cultures were isolated. On the basis of research into morphological, tinctorial, cultural and physiological and biochemical properties of the isolated strains, they were identified to species or genus level. Of 22 isolated strains of the enteric bacilli group, Escherichia coli accounted for 9.1%, Enterobacter agglomerans, 50%, Citrobacter freundii, 27.3%, C. diversus, 9.1%, C. amalonaticus 4.5%. Among enterococci, mainly Enterococcus faecium were determined, E. faecalis accounting for from 8 to 25% of the enterococci group. Bacteria of the genera Aeromonas, Pseudomonas, Bacillus, Vibrio, and a proteolytic biovariety of enterococcus were most active hydrolase producers. Among aerobic bacteria, Bacillus cereus were identified; anaerobic bacteria were represented mainly by Clostridium perfringens.

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Bibliographic Details
Main Author: Puchenkova, S.G.
Other Authors: Yakovlev, V.N.
Format: Journal Contribution biblioteca
Language:Russian
Published: 1994
Subjects:Taxonomy, Bacterioflora, Microorganisms, Biological sedimentation, Enterobacter agglomerans, Citrobacter freundii, Escherichia соli, Bacillus cereus, Biotechnologies, Таксономия, Микроорганизмы, Биоотложения, Анаэробные бактерии, Энтерококки, Биотехника,
Online Access:http://hdl.handle.net/1834/15827
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spelling dig-aquadocs-1834-158272021-05-19T06:59:31Z Characteristic of bacterial communities associated with mollusc biodeposits Характеристика бактериальных сообществ, ассоциированных с биоотложениями моллюсков Puchenkova, S.G. Yakovlev, V.N. Taxonomy Bacterioflora Microorganisms Biological sedimentation Enterobacter agglomerans Citrobacter freundii Escherichia соli Bacillus cereus Biotechnologies Таксономия Микроорганизмы Биоотложения Анаэробные бактерии Энтерококки Биотехника Quantitative and qualitative composition of the bacterioflora of biosediments produced by mussels and oysters were studied in the southern Kerch Strait, area of potential experimental and commercial culture of molluscs. Sampling was made from May to October 1993. A total of 45 various groups of microorganisms were determined and 50 pure bacterial cultures were isolated. On the basis of research into morphological, tinctorial, cultural and physiological and biochemical properties of the isolated strains, they were identified to species or genus level. Of 22 isolated strains of the enteric bacilli group, Escherichia coli accounted for 9.1%, Enterobacter agglomerans, 50%, Citrobacter freundii, 27.3%, C. diversus, 9.1%, C. amalonaticus 4.5%. Among enterococci, mainly Enterococcus faecium were determined, E. faecalis accounting for from 8 to 25% of the enterococci group. Bacteria of the genera Aeromonas, Pseudomonas, Bacillus, Vibrio, and a proteolytic biovariety of enterococcus were most active hydrolase producers. Among aerobic bacteria, Bacillus cereus were identified; anaerobic bacteria were represented mainly by Clostridium perfringens. Выявлен обильный рост гетеротрофной (сапрофитной) микрофлоры. Сапрофитные бактерии используют органическое вещество биоотложений в своём конструктивном и энергетическом обмене их количество напрямую связано с наличием легкоусвояемых соединений углерода и азота, поэтому они могут рассматриваться как достаточно информативные биологические индикаторы процессов самоочищения среды. По отдельности определяли количество психрофильных бактерий с температурным оптимумом роста 20-22°С и мезофильных — растущих при температуре инкубации 37°С. Психрофилы в основном состоят из представителей морской флоры, циркулирующих в водной среде и организме гидробионтов. Среди мезофилов доминируют терригенные бактерии, поступающие в прибрежную зону моря с различными источниками антропогенного загрязнения и адаптированные к новым условиям существования. Сезонная динамика этих двух групп сапрофитов в биоотложениях моллюсков района размещения мидий- ных установок представлена на графиках. Общепринятые показатели санитарно-гигиенического и эпидемического состояния объектов внешней среды — бактерии группы кишечных палочек (БГКП) и энтерококки были обнаружены в среднем количестве 240 НВЧ/г. Численность энтерококков в биоотложениях была почти постоянной (за исключением единичного случая), БГКП — более вариабельна. БГКП были представлены в основном цитратассимилируюшими видами. Из 22 выделенных штаммов БГКП Escherichia соli составили 9,1%, Enterobacter agglomerans — 50,0%, Citrobacter freundii — 27,3%, С. diversus — 9,1%, С, amalonaticus — 4,5%. Среди энтерококков определены в основном Enterococcus faecium, а количество Е. faecalis составляло в общей группе энтерококков от 8 до 25%. Таким образом, показатели фекального загрязнения были обнаружены в биоотложениях в невысоких количествах. Published 2019-11-21T08:54:54Z 2019-11-21T08:54:54Z 1994 Journal Contribution Non Refereed УДК 579.8+594 http://hdl.handle.net/1834/15827 ru pp.120-123 Kerch Strait Керченский пролив
institution UNESCO
collection DSpace
country Francia
countrycode FR
component Bibliográfico
access En linea
databasecode dig-aquadocs
tag biblioteca
region Europa del Oeste
libraryname Repositorio AQUADOCS
language Russian
topic Taxonomy
Bacterioflora
Microorganisms
Biological sedimentation
Enterobacter agglomerans
Citrobacter freundii
Escherichia соli
Bacillus cereus
Biotechnologies
Таксономия
Микроорганизмы
Биоотложения
Анаэробные бактерии
Энтерококки
Биотехника
Taxonomy
Bacterioflora
Microorganisms
Biological sedimentation
Enterobacter agglomerans
Citrobacter freundii
Escherichia соli
Bacillus cereus
Biotechnologies
Таксономия
Микроорганизмы
Биоотложения
Анаэробные бактерии
Энтерококки
Биотехника
spellingShingle Taxonomy
Bacterioflora
Microorganisms
Biological sedimentation
Enterobacter agglomerans
Citrobacter freundii
Escherichia соli
Bacillus cereus
Biotechnologies
Таксономия
Микроорганизмы
Биоотложения
Анаэробные бактерии
Энтерококки
Биотехника
Taxonomy
Bacterioflora
Microorganisms
Biological sedimentation
Enterobacter agglomerans
Citrobacter freundii
Escherichia соli
Bacillus cereus
Biotechnologies
Таксономия
Микроорганизмы
Биоотложения
Анаэробные бактерии
Энтерококки
Биотехника
Puchenkova, S.G.
Characteristic of bacterial communities associated with mollusc biodeposits
description Quantitative and qualitative composition of the bacterioflora of biosediments produced by mussels and oysters were studied in the southern Kerch Strait, area of potential experimental and commercial culture of molluscs. Sampling was made from May to October 1993. A total of 45 various groups of microorganisms were determined and 50 pure bacterial cultures were isolated. On the basis of research into morphological, tinctorial, cultural and physiological and biochemical properties of the isolated strains, they were identified to species or genus level. Of 22 isolated strains of the enteric bacilli group, Escherichia coli accounted for 9.1%, Enterobacter agglomerans, 50%, Citrobacter freundii, 27.3%, C. diversus, 9.1%, C. amalonaticus 4.5%. Among enterococci, mainly Enterococcus faecium were determined, E. faecalis accounting for from 8 to 25% of the enterococci group. Bacteria of the genera Aeromonas, Pseudomonas, Bacillus, Vibrio, and a proteolytic biovariety of enterococcus were most active hydrolase producers. Among aerobic bacteria, Bacillus cereus were identified; anaerobic bacteria were represented mainly by Clostridium perfringens.
author2 Yakovlev, V.N.
author_facet Yakovlev, V.N.
Puchenkova, S.G.
format Journal Contribution
topic_facet Taxonomy
Bacterioflora
Microorganisms
Biological sedimentation
Enterobacter agglomerans
Citrobacter freundii
Escherichia соli
Bacillus cereus
Biotechnologies
Таксономия
Микроорганизмы
Биоотложения
Анаэробные бактерии
Энтерококки
Биотехника
author Puchenkova, S.G.
author_sort Puchenkova, S.G.
title Characteristic of bacterial communities associated with mollusc biodeposits
title_short Characteristic of bacterial communities associated with mollusc biodeposits
title_full Characteristic of bacterial communities associated with mollusc biodeposits
title_fullStr Characteristic of bacterial communities associated with mollusc biodeposits
title_full_unstemmed Characteristic of bacterial communities associated with mollusc biodeposits
title_sort characteristic of bacterial communities associated with mollusc biodeposits
publishDate 1994
url http://hdl.handle.net/1834/15827
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