Evaluation of incubation temperatures on DNA damages of the Caspian trout (Salmo trutta caspius Kessler, 1877) single cells through developmental stages by Comet assay
Caspian trout (Salmo trutta caspius Kessler, 1877) as endemic migratory fish, possessing great ecological economic and biodiversity value. Among the habitat drivers, temperature related effects on the genome were considered in this research. During the embryonic developmental stages, genomic alterations of single cells were assessed by Comet assay. After artificial propagation of wild broodstocks, the batches of eggs, were transferred to incubators with constant temperatures, REDD water recycling, 3 stages water filtration, UV-C application on effluent of each incubator and DO–pH–temperature control. Samples were collected from normal (8°C), cold (4°C) and warm (12°C) treatments on eyed egg, alevin and larval stages. Single Cell Gel Electrophoresis were applied on samples for evaluation of Comet Tail length (TL), DNA in tail (DT) and Tail Moment (TM) indices as DNA damage markers. Comparison between mean values of Comet TL index indicated 85.19 ±9.2, 9.88 ±2.3 and 256.54±14.6 for 4, 8 and 12°C treatments, respectively. DT were 18.07±6.3, 9.72±1.9 and 31.47±2.8 and then TM were measured as 15.27± 4.1, 1.21±0.2 and 95.25±8.6 for 4, 8, and 12°C treatments, respectively. Significant difference between thermal treatments (p<0.05) observed. Warm treatments demonstrated greater DNA damage in compare with others. TM explained better information in comparison with TL and DT in all analyzed samples. In conclusion, temperatures out of the range of the optimal conditions could significantly affect Caspian trout genome which could be the subject of management considerations pertaining to Caspian trout stocks rehabilitation and captive rearing strategies.
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Format: | Journal Contribution biblioteca |
Language: | English |
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2019
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Subjects: | Ecological, Caspian trout, Salmo trutta caspius, Temperature, DNA damage, Comet assay, Evaluation, |
Online Access: | http://hdl.handle.net/1834/15086 |
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dig-aquadocs-1834-150862021-05-19T06:41:32Z Evaluation of incubation temperatures on DNA damages of the Caspian trout (Salmo trutta caspius Kessler, 1877) single cells through developmental stages by Comet assay Alizadeh Sabet, H.R. Kalbassi, M.R. Pourkazemi, M. Sadeghizadeh, M. Ecological Caspian trout Salmo trutta caspius Temperature DNA damage Comet assay Evaluation Caspian trout (Salmo trutta caspius Kessler, 1877) as endemic migratory fish, possessing great ecological economic and biodiversity value. Among the habitat drivers, temperature related effects on the genome were considered in this research. During the embryonic developmental stages, genomic alterations of single cells were assessed by Comet assay. After artificial propagation of wild broodstocks, the batches of eggs, were transferred to incubators with constant temperatures, REDD water recycling, 3 stages water filtration, UV-C application on effluent of each incubator and DO–pH–temperature control. Samples were collected from normal (8°C), cold (4°C) and warm (12°C) treatments on eyed egg, alevin and larval stages. Single Cell Gel Electrophoresis were applied on samples for evaluation of Comet Tail length (TL), DNA in tail (DT) and Tail Moment (TM) indices as DNA damage markers. Comparison between mean values of Comet TL index indicated 85.19 ±9.2, 9.88 ±2.3 and 256.54±14.6 for 4, 8 and 12°C treatments, respectively. DT were 18.07±6.3, 9.72±1.9 and 31.47±2.8 and then TM were measured as 15.27± 4.1, 1.21±0.2 and 95.25±8.6 for 4, 8, and 12°C treatments, respectively. Significant difference between thermal treatments (p<0.05) observed. Warm treatments demonstrated greater DNA damage in compare with others. TM explained better information in comparison with TL and DT in all analyzed samples. In conclusion, temperatures out of the range of the optimal conditions could significantly affect Caspian trout genome which could be the subject of management considerations pertaining to Caspian trout stocks rehabilitation and captive rearing strategies. Published 2019-04-18T06:54:36Z 2019-04-18T06:54:36Z 2019 Journal Contribution Refereed 1562-2916 10.22092/ijfs.2018.117623 http://hdl.handle.net/1834/15086 en pp.48-59 Iran |
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Ecological Caspian trout Salmo trutta caspius Temperature DNA damage Comet assay Evaluation Ecological Caspian trout Salmo trutta caspius Temperature DNA damage Comet assay Evaluation |
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Ecological Caspian trout Salmo trutta caspius Temperature DNA damage Comet assay Evaluation Ecological Caspian trout Salmo trutta caspius Temperature DNA damage Comet assay Evaluation Alizadeh Sabet, H.R. Kalbassi, M.R. Pourkazemi, M. Sadeghizadeh, M. Evaluation of incubation temperatures on DNA damages of the Caspian trout (Salmo trutta caspius Kessler, 1877) single cells through developmental stages by Comet assay |
description |
Caspian trout (Salmo trutta caspius Kessler, 1877) as endemic migratory fish, possessing great
ecological economic and biodiversity value. Among the habitat drivers, temperature related
effects on the genome were considered in this research. During the embryonic developmental
stages, genomic alterations of single cells were assessed by Comet assay. After artificial
propagation of wild broodstocks, the batches of eggs, were transferred to incubators with
constant temperatures, REDD water recycling, 3 stages water filtration, UV-C application on
effluent of each incubator and DO–pH–temperature control. Samples were collected from
normal (8°C), cold (4°C) and warm (12°C) treatments on eyed egg, alevin and larval stages.
Single Cell Gel Electrophoresis were applied on samples for evaluation of Comet Tail length
(TL), DNA in tail (DT) and Tail Moment (TM) indices as DNA damage markers. Comparison
between mean values of Comet TL index indicated 85.19 ±9.2, 9.88 ±2.3 and 256.54±14.6 for
4, 8 and 12°C treatments, respectively. DT were 18.07±6.3, 9.72±1.9 and 31.47±2.8 and then
TM were measured as 15.27± 4.1, 1.21±0.2 and 95.25±8.6 for 4, 8, and 12°C treatments,
respectively. Significant difference between thermal treatments (p<0.05) observed. Warm
treatments demonstrated greater DNA damage in compare with others. TM explained better
information in comparison with TL and DT in all analyzed samples. In conclusion,
temperatures out of the range of the optimal conditions could significantly affect Caspian trout
genome which could be the subject of management considerations pertaining to Caspian trout
stocks rehabilitation and captive rearing strategies. |
format |
Journal Contribution |
topic_facet |
Ecological Caspian trout Salmo trutta caspius Temperature DNA damage Comet assay Evaluation |
author |
Alizadeh Sabet, H.R. Kalbassi, M.R. Pourkazemi, M. Sadeghizadeh, M. |
author_facet |
Alizadeh Sabet, H.R. Kalbassi, M.R. Pourkazemi, M. Sadeghizadeh, M. |
author_sort |
Alizadeh Sabet, H.R. |
title |
Evaluation of incubation temperatures on DNA damages of the Caspian trout (Salmo trutta caspius Kessler, 1877) single cells through developmental stages by Comet assay |
title_short |
Evaluation of incubation temperatures on DNA damages of the Caspian trout (Salmo trutta caspius Kessler, 1877) single cells through developmental stages by Comet assay |
title_full |
Evaluation of incubation temperatures on DNA damages of the Caspian trout (Salmo trutta caspius Kessler, 1877) single cells through developmental stages by Comet assay |
title_fullStr |
Evaluation of incubation temperatures on DNA damages of the Caspian trout (Salmo trutta caspius Kessler, 1877) single cells through developmental stages by Comet assay |
title_full_unstemmed |
Evaluation of incubation temperatures on DNA damages of the Caspian trout (Salmo trutta caspius Kessler, 1877) single cells through developmental stages by Comet assay |
title_sort |
evaluation of incubation temperatures on dna damages of the caspian trout (salmo trutta caspius kessler, 1877) single cells through developmental stages by comet assay |
publishDate |
2019 |
url |
http://hdl.handle.net/1834/15086 |
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