Population Structure of Caspian Roach (Rutilus Rutilus caspicus) in Southern Coasts of Caspian Sea using Microsatellite Maker
Caspian roach is regarded as a valuable bony fish that has economic importance in southern parts of Caspian Sea. Investigations show that natural reproduction of this species has been declining during recent years. Restocking of this fish is achieved by the way of artificial reproduction. Loss of genetic stock of this species is of great concern. In the current study, in order to study the population structure of roach, 54 individuals were captured from Gomishan and Anzali wetlands (27 individuals from each region) in April 2007. Genomic DNA was extracted using phenol-chloroform method and investigated by 10 microsatellite loci. The genetic diversity of two regions [(Gomishan: average number of alleles, Na= 11.4, average effective number of alleles, Ne= 7.92, observed heterozygosity, Ho= 0.69 and expected heterozygosity, He= 0.85) (Anzali: Na= 10.2, Ne= 7.38, Ho= 0.71 and He= 0.84)] were not statistically different (P>0.05). Analysis of molecular variance showed high genetic diversity (%95) within populations. The Fst value was 0.046, indicating low genetic differentiation among Anzali and Gomishan regions. In this study, 10 of 20 (10 loci × 2 populations) tests showed significant deviation (P≤ 0.005) from Hardy-Weinberg equilibrium due to the heterozygosity deficiency. Results from UPGMA cluster analysis, based on Nei´s genetic distance, showed that the regions are probably separated from each other.
Main Authors: | , , , |
---|---|
Format: | Journal Contribution biblioteca |
Language: | Persian |
Published: |
2012
|
Subjects: | Rutilus rutilus caspicus, Caspian Roach, Microsatellite, Fish, Population, Structure, |
Online Access: | http://hdl.handle.net/1834/14662 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-aquadocs-1834-14662 |
---|---|
record_format |
koha |
spelling |
dig-aquadocs-1834-146622021-05-19T06:53:19Z Population Structure of Caspian Roach (Rutilus Rutilus caspicus) in Southern Coasts of Caspian Sea using Microsatellite Maker Kashiri, Hadis Shabani, Ali Shabanpour, Bahareh Razaei, Mohammad Rutilus rutilus caspicus Caspian Roach Microsatellite Fish Population Structure Caspian roach is regarded as a valuable bony fish that has economic importance in southern parts of Caspian Sea. Investigations show that natural reproduction of this species has been declining during recent years. Restocking of this fish is achieved by the way of artificial reproduction. Loss of genetic stock of this species is of great concern. In the current study, in order to study the population structure of roach, 54 individuals were captured from Gomishan and Anzali wetlands (27 individuals from each region) in April 2007. Genomic DNA was extracted using phenol-chloroform method and investigated by 10 microsatellite loci. The genetic diversity of two regions [(Gomishan: average number of alleles, Na= 11.4, average effective number of alleles, Ne= 7.92, observed heterozygosity, Ho= 0.69 and expected heterozygosity, He= 0.85) (Anzali: Na= 10.2, Ne= 7.38, Ho= 0.71 and He= 0.84)] were not statistically different (P>0.05). Analysis of molecular variance showed high genetic diversity (%95) within populations. The Fst value was 0.046, indicating low genetic differentiation among Anzali and Gomishan regions. In this study, 10 of 20 (10 loci × 2 populations) tests showed significant deviation (P≤ 0.005) from Hardy-Weinberg equilibrium due to the heterozygosity deficiency. Results from UPGMA cluster analysis, based on Nei´s genetic distance, showed that the regions are probably separated from each other. Published 2018-10-09T12:21:08Z 2018-10-09T12:21:08Z 2012 Journal Contribution Refereed 2008-8965 http://hdl.handle.net/1834/14662 fa pp.4-14 Iran Caspian Sea |
institution |
UNESCO |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-aquadocs |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Repositorio AQUADOCS |
language |
Persian |
topic |
Rutilus rutilus caspicus Caspian Roach Microsatellite Fish Population Structure Rutilus rutilus caspicus Caspian Roach Microsatellite Fish Population Structure |
spellingShingle |
Rutilus rutilus caspicus Caspian Roach Microsatellite Fish Population Structure Rutilus rutilus caspicus Caspian Roach Microsatellite Fish Population Structure Kashiri, Hadis Shabani, Ali Shabanpour, Bahareh Razaei, Mohammad Population Structure of Caspian Roach (Rutilus Rutilus caspicus) in Southern Coasts of Caspian Sea using Microsatellite Maker |
description |
Caspian roach is regarded as a valuable bony fish that has economic importance in southern parts of Caspian Sea. Investigations show that natural reproduction of this species has been declining during recent years. Restocking of this fish is achieved by the way of artificial reproduction. Loss of genetic stock of this species is of great concern. In the current study, in order to study the population structure of roach, 54 individuals were captured from Gomishan and Anzali wetlands (27 individuals from each region) in April 2007. Genomic DNA was extracted using phenol-chloroform method and investigated by 10 microsatellite loci. The genetic diversity of two regions [(Gomishan: average number of alleles, Na= 11.4, average effective number of alleles, Ne= 7.92, observed heterozygosity, Ho= 0.69 and expected heterozygosity, He= 0.85) (Anzali: Na= 10.2, Ne= 7.38, Ho= 0.71 and He= 0.84)] were not statistically different (P>0.05). Analysis of molecular variance showed high genetic diversity (%95) within populations. The Fst value was 0.046, indicating low genetic differentiation among Anzali and Gomishan regions. In this study, 10 of 20 (10 loci × 2 populations) tests showed significant deviation (P≤ 0.005) from Hardy-Weinberg equilibrium due to the heterozygosity deficiency. Results from UPGMA cluster analysis, based on Nei´s genetic distance, showed that the regions are probably separated from each other. |
format |
Journal Contribution |
topic_facet |
Rutilus rutilus caspicus Caspian Roach Microsatellite Fish Population Structure |
author |
Kashiri, Hadis Shabani, Ali Shabanpour, Bahareh Razaei, Mohammad |
author_facet |
Kashiri, Hadis Shabani, Ali Shabanpour, Bahareh Razaei, Mohammad |
author_sort |
Kashiri, Hadis |
title |
Population Structure of Caspian Roach (Rutilus Rutilus caspicus) in Southern Coasts of Caspian Sea using Microsatellite Maker |
title_short |
Population Structure of Caspian Roach (Rutilus Rutilus caspicus) in Southern Coasts of Caspian Sea using Microsatellite Maker |
title_full |
Population Structure of Caspian Roach (Rutilus Rutilus caspicus) in Southern Coasts of Caspian Sea using Microsatellite Maker |
title_fullStr |
Population Structure of Caspian Roach (Rutilus Rutilus caspicus) in Southern Coasts of Caspian Sea using Microsatellite Maker |
title_full_unstemmed |
Population Structure of Caspian Roach (Rutilus Rutilus caspicus) in Southern Coasts of Caspian Sea using Microsatellite Maker |
title_sort |
population structure of caspian roach (rutilus rutilus caspicus) in southern coasts of caspian sea using microsatellite maker |
publishDate |
2012 |
url |
http://hdl.handle.net/1834/14662 |
work_keys_str_mv |
AT kashirihadis populationstructureofcaspianroachrutilusrutiluscaspicusinsoutherncoastsofcaspianseausingmicrosatellitemaker AT shabaniali populationstructureofcaspianroachrutilusrutiluscaspicusinsoutherncoastsofcaspianseausingmicrosatellitemaker AT shabanpourbahareh populationstructureofcaspianroachrutilusrutiluscaspicusinsoutherncoastsofcaspianseausingmicrosatellitemaker AT razaeimohammad populationstructureofcaspianroachrutilusrutiluscaspicusinsoutherncoastsofcaspianseausingmicrosatellitemaker |
_version_ |
1756076590009155584 |