Investigation of possibility of identification sex marker in beluga (Huso huso) and Persian sturgeon (Acipenser persicus) by using of molecular techniques
Due to high maturation age in sturgeons and lack of morphologic differences between male and female even in brood stocks, sex determination is difficult in these species. In this research with using of AFLP approach and 100 primer combinations, male and female genomic DNA of 20 individuals in Persian sturgeon (Acipenser persicus) and beluga (Huso huso) were investigated. Ligation was carried out with using of MseI and EcoRI, and then adapters were ligated with using of T4 DNA Ligase. Fragments amplification was done through two steps PCR and electrophoresis on denature poly acrylamid and stained by silver staining. Data derived from banding patterns were scored as o (absence) and 1 (Presence). A set of 100 (Eco+3 and Mse+4) primer combinations in A. persicus and H. huso yielded approximately a total of 3771 and 3779 scorable bands, respectively of which 30% in A. persicus and 29.6% in H. huso were polymorphic. The fragments ranged from 50 to 600 bp without revealing any sex specific markers. So we used cDNA-AFLP approach in order to analysis of gene expression in 8 female and 8 male Persian sturgeon gonads. Results revealed two cDNA markers in female gonad (TDF1, TDF2) and they verified with RT-PCR in male and female gonads cDNA. But unfortunately they didn t verify in genomic DNA. According to this research results and previous researches, it seems that sturgeons may have not sex chromosomes or the methods were used couldn t determine them.
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Format: | Report biblioteca |
Language: | Persian |
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Iranian Fisheries Science Research Institute
2009
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Subjects: | Persian sturgeon, Beluga, Sex marker, AFLP, cDNA-AFLP, Investigation, Identification, Huso huso, Acipenser persicus, Molecular, |
Online Access: | http://hdl.handle.net/1834/14085 |
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dig-aquadocs-1834-140852021-05-19T06:49:15Z Investigation of possibility of identification sex marker in beluga (Huso huso) and Persian sturgeon (Acipenser persicus) by using of molecular techniques Yarmohammadi, Mahtab Pourkazemi, M. Hassanzadeh Saber, M. Chakmehdooz, F. Baradaran Novairi, Sh. Fashkhami, M.R. Mardi, M. Persian sturgeon Beluga Sex marker AFLP cDNA-AFLP Investigation Identification Huso huso Acipenser persicus Molecular Due to high maturation age in sturgeons and lack of morphologic differences between male and female even in brood stocks, sex determination is difficult in these species. In this research with using of AFLP approach and 100 primer combinations, male and female genomic DNA of 20 individuals in Persian sturgeon (Acipenser persicus) and beluga (Huso huso) were investigated. Ligation was carried out with using of MseI and EcoRI, and then adapters were ligated with using of T4 DNA Ligase. Fragments amplification was done through two steps PCR and electrophoresis on denature poly acrylamid and stained by silver staining. Data derived from banding patterns were scored as o (absence) and 1 (Presence). A set of 100 (Eco+3 and Mse+4) primer combinations in A. persicus and H. huso yielded approximately a total of 3771 and 3779 scorable bands, respectively of which 30% in A. persicus and 29.6% in H. huso were polymorphic. The fragments ranged from 50 to 600 bp without revealing any sex specific markers. So we used cDNA-AFLP approach in order to analysis of gene expression in 8 female and 8 male Persian sturgeon gonads. Results revealed two cDNA markers in female gonad (TDF1, TDF2) and they verified with RT-PCR in male and female gonads cDNA. But unfortunately they didn t verify in genomic DNA. According to this research results and previous researches, it seems that sturgeons may have not sex chromosomes or the methods were used couldn t determine them. Iranian Fisheries Science Research Institute Published 2018-08-15T08:19:44Z 2018-08-15T08:19:44Z 2009 Report Refereed http://hdl.handle.net/1834/14085 fa 2009.618; 112pp. Iran Iranian Fisheries Science Research Institute Tehran, Iran |
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Persian sturgeon Beluga Sex marker AFLP cDNA-AFLP Investigation Identification Huso huso Acipenser persicus Molecular Persian sturgeon Beluga Sex marker AFLP cDNA-AFLP Investigation Identification Huso huso Acipenser persicus Molecular |
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Persian sturgeon Beluga Sex marker AFLP cDNA-AFLP Investigation Identification Huso huso Acipenser persicus Molecular Persian sturgeon Beluga Sex marker AFLP cDNA-AFLP Investigation Identification Huso huso Acipenser persicus Molecular Yarmohammadi, Mahtab Pourkazemi, M. Hassanzadeh Saber, M. Chakmehdooz, F. Baradaran Novairi, Sh. Fashkhami, M.R. Mardi, M. Investigation of possibility of identification sex marker in beluga (Huso huso) and Persian sturgeon (Acipenser persicus) by using of molecular techniques |
description |
Due to high maturation age in sturgeons and lack of morphologic differences between male and female even in
brood stocks, sex determination is difficult in these species. In this research with using of AFLP approach and
100 primer combinations, male and female genomic DNA of 20 individuals in Persian sturgeon (Acipenser
persicus) and beluga (Huso huso) were investigated. Ligation was carried out with using of MseI and EcoRI, and
then adapters were ligated with using of T4 DNA Ligase. Fragments amplification was done through two steps
PCR and electrophoresis on denature poly acrylamid and stained by silver staining. Data derived from banding
patterns were scored as o (absence) and 1 (Presence). A set of 100 (Eco+3 and Mse+4) primer combinations in
A. persicus and H. huso yielded approximately a total of 3771 and 3779 scorable bands, respectively of which
30% in A. persicus and 29.6% in H. huso were polymorphic. The fragments ranged from 50 to 600 bp without
revealing any sex specific markers. So we used cDNA-AFLP approach in order to analysis of gene expression
in 8 female and 8 male Persian sturgeon gonads. Results revealed two cDNA markers in female gonad (TDF1, TDF2) and they verified with RT-PCR in male and female gonads cDNA. But unfortunately they didn t verify in
genomic DNA. According to this research results and previous researches, it seems that sturgeons may have not
sex chromosomes or the methods were used couldn t determine them. |
format |
Report |
topic_facet |
Persian sturgeon Beluga Sex marker AFLP cDNA-AFLP Investigation Identification Huso huso Acipenser persicus Molecular |
author |
Yarmohammadi, Mahtab Pourkazemi, M. Hassanzadeh Saber, M. Chakmehdooz, F. Baradaran Novairi, Sh. Fashkhami, M.R. Mardi, M. |
author_facet |
Yarmohammadi, Mahtab Pourkazemi, M. Hassanzadeh Saber, M. Chakmehdooz, F. Baradaran Novairi, Sh. Fashkhami, M.R. Mardi, M. |
author_sort |
Yarmohammadi, Mahtab |
title |
Investigation of possibility of identification sex marker in beluga (Huso huso) and Persian sturgeon (Acipenser persicus) by using of molecular techniques |
title_short |
Investigation of possibility of identification sex marker in beluga (Huso huso) and Persian sturgeon (Acipenser persicus) by using of molecular techniques |
title_full |
Investigation of possibility of identification sex marker in beluga (Huso huso) and Persian sturgeon (Acipenser persicus) by using of molecular techniques |
title_fullStr |
Investigation of possibility of identification sex marker in beluga (Huso huso) and Persian sturgeon (Acipenser persicus) by using of molecular techniques |
title_full_unstemmed |
Investigation of possibility of identification sex marker in beluga (Huso huso) and Persian sturgeon (Acipenser persicus) by using of molecular techniques |
title_sort |
investigation of possibility of identification sex marker in beluga (huso huso) and persian sturgeon (acipenser persicus) by using of molecular techniques |
publisher |
Iranian Fisheries Science Research Institute |
publishDate |
2009 |
url |
http://hdl.handle.net/1834/14085 |
work_keys_str_mv |
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