Survey of different dietary energy and protein levels on the reproduction indices of Benni (Barbus sharpeyi) broodstock

The survey of different protein and energy levels of brooders Benni(Mesopotamichthys sharpeyi) diet on reproductive indices This study,to preparation of suitable diet for brooders of Benni(Mesopotamichthys sharpeyi) carried Out increasing efficiency artificial propagation and achievement to maximum working fecundity and fingerling production. 9 dietary feeding with 3 triplication were prepared that included 3 protein levels(30,35 and 40 percentage) and 3 different levels of digestable energy (250,300 and 350 kcal/100g).The reproductive indices (working fecundity, fertilization, hatching and survival rate) were studied in this experiment in South Iran Aquaculture Research Center .12 female brooders in any triplication were transported to earthern ponds 300 m2. They were fed 2 times in days for 4 month until satiation.The artificial breeding carried out in spring for survey of reproductive indices. The amount of hypophysis injection was 3mg/kg weight of fish with two doses10% in first stage and 90% in second stage with interval 10 Hour . The male broodstock injection was first stage spantanously with second females injections with a dose of 2 mg/kg.The total of breeding stages until larvae release was registered 22.5-24.5°C. Result showed that constant protein (35%) with rising energy until specified amount(350 Kcal/100g) was increased reproductive indicies.The working fecundity is one of the reproductive indices that in treatment 5 had significant difference compare to other treatment.In this survey, Comparison between different levels of dietary protein and energy on the brooders Benni indicated that 35% protein level and 350 Kcal/100g energy for barbus grypus had best efficiency in diet of broodstock.