Preliminary study on induction of triploidy in Benni (Barbus sharpeyi) by thermal shocks

Barbus sharpeyi is a local fish of Khouzestan that is planned to be a target for aquaculture in recent programs of fisheries organization. Considering importance of this species in its dispersal region, the main goal of this project was evaluation of possibility for triploidy induction and its potential in response to the heat shocks, efficiency of viability and growth and finally reporting the best condition for triploidy induction in Benny. Induction of thermal shocks was executed in for cold and heat shocks (2 and 4°C for cold and 34, 36 and 38°C for heat). Time of induction and its duration varied between 2 and 5 minutes after the fertilization for 3 and 5 minutes. Each treatment was repeated for 3 times. The ploidy level was determined based on size of nucleus diameters in erythrocytes. Analysis of data was done by SPSS (ver. 16) using T-test and ANOVA method. Results showed that the maximum number of triploid individuals was obtained in treatment of 38°C, 2 min after the fertilization by duration of 3 minutes but as the condition was not suitable for the viability of the eggs, losses of the larvae was high in this group. The best efficiency of triploidization in B. sharpeyi belongs to the 34°C, 2 to 5 minutes after the fertilization for duration of 5 minutes. Nuclear dimensions showed an increase in triploids and confirmed that this characteristic can be used as a reliable factor to distinguish polyploidy. Results of this study showed that B. sharpeyi has the ability for polyploidy inductions specially heat shocks. Evaluation of growth in matured fishes, use of proper tagging systems to distinguish the treatments and designing a plan for bioconserving and genetic improvement of this species is recommended.

Bibliographic Details

Main Authors:	Jorfi, Elham, Mortazavizadeh, S.A.S., Sanjari, M., Amiri, F., Youneszadeh, M.F., Nikpey, M., Eskandari, Gh., Basak Kahkesh, F.
Format:	Report biblioteca
Language:	Persian
Published:	Iranian Fisheries Science Research Institute 2016
Subjects:	Aquaculture, Triploidy, Benni, Barbus sharpeyi, Thermal shocks, Species, Growth, Fertilization, ANOVA, Bio conserving, Genetic,
Online Access:	http://hdl.handle.net/1834/13762
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