Study of total hemocyte count and total plasma protein in immune system of freshwater Crayfish (Astacus leptodactylus)
In this study, hemocytes defence cells and total plasma protein had been studied in immune system of freshwater Crayfish Astacus leptodactylus. About one-hundered freshwater Crayfish (A.leptodactylus) with average weight of 25-40g were purchased from aras damreservours in west Azarbayjan province and transported to Iranian artemia research center of orumya province.(25 pieces of Crayfish were considered surplus for possible losses) Before experimernt the Crayfish were acclimated for two days in the laboratory and then disinfected by oxytetracycline antibiotic and finally, an equal number (15 pieces of Crayfish in each glass aquariums) transported to 5 glass aquariums. In order to study hemocyte and plasma total protein, the hemolymph samples were withdrawn from abdominal second segments of Crayfish in interval hours (0, 6, 12, 24, 48, 96, 144, 240 and 336). Result showed that the mean of hemocytes was 27-114 CFU ml-1 , and the highest and lowest value of hemocytes were 180 and 12 CFU ml-1 in 12 and 336 hours after experiment times, respectively. Also based on the results, the semigranular cells(SGC) and granular cells(GC) were approximately comprised about 58-65% and 28- 37%, respectively and hyaline cells(HC) about was 3-6%. Based on total plasma protein results, the mean of total plasma protein was 1-2 Gr dl-1 , and the highest and lowest value of total plasma protein were 2/8 and 0/5 Gr dl-1 in 12 and 336 hours after experiment times.
Main Authors: | , |
---|---|
Format: | Journal Contribution biblioteca |
Language: | Persian |
Published: |
2016
|
Subjects: | Hemocytes, Granular cells, Semigranular cells, Hyaline cells, Total plasma protein, Astacus leptodactylus, Crayfish, Fresh waters, Plasma, Protein, Immune, Glass aquarium, |
Online Access: | http://hdl.handle.net/1834/13633 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-aquadocs-1834-13633 |
---|---|
record_format |
koha |
spelling |
dig-aquadocs-1834-136332021-05-19T06:53:53Z Study of total hemocyte count and total plasma protein in immune system of freshwater Crayfish (Astacus leptodactylus) Samnejhad, Arash Afsharnasab, Mohammad Hemocytes Granular cells Semigranular cells Hyaline cells Total plasma protein Astacus leptodactylus Crayfish Fresh waters Plasma Protein Immune Glass aquarium In this study, hemocytes defence cells and total plasma protein had been studied in immune system of freshwater Crayfish Astacus leptodactylus. About one-hundered freshwater Crayfish (A.leptodactylus) with average weight of 25-40g were purchased from aras damreservours in west Azarbayjan province and transported to Iranian artemia research center of orumya province.(25 pieces of Crayfish were considered surplus for possible losses) Before experimernt the Crayfish were acclimated for two days in the laboratory and then disinfected by oxytetracycline antibiotic and finally, an equal number (15 pieces of Crayfish in each glass aquariums) transported to 5 glass aquariums. In order to study hemocyte and plasma total protein, the hemolymph samples were withdrawn from abdominal second segments of Crayfish in interval hours (0, 6, 12, 24, 48, 96, 144, 240 and 336). Result showed that the mean of hemocytes was 27-114 CFU ml-1 , and the highest and lowest value of hemocytes were 180 and 12 CFU ml-1 in 12 and 336 hours after experiment times, respectively. Also based on the results, the semigranular cells(SGC) and granular cells(GC) were approximately comprised about 58-65% and 28- 37%, respectively and hyaline cells(HC) about was 3-6%. Based on total plasma protein results, the mean of total plasma protein was 1-2 Gr dl-1 , and the highest and lowest value of total plasma protein were 2/8 and 0/5 Gr dl-1 in 12 and 336 hours after experiment times. Published 2018-08-04T05:42:22Z 2018-08-04T05:42:22Z 2016 Journal Contribution Refereed 2008-8965 http://hdl.handle.net/1834/13633 fa pp.49-58 Iran West Azarbayjan province |
institution |
UNESCO |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-aquadocs |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Repositorio AQUADOCS |
language |
Persian |
topic |
Hemocytes Granular cells Semigranular cells Hyaline cells Total plasma protein Astacus leptodactylus Crayfish Fresh waters Plasma Protein Immune Glass aquarium Hemocytes Granular cells Semigranular cells Hyaline cells Total plasma protein Astacus leptodactylus Crayfish Fresh waters Plasma Protein Immune Glass aquarium |
spellingShingle |
Hemocytes Granular cells Semigranular cells Hyaline cells Total plasma protein Astacus leptodactylus Crayfish Fresh waters Plasma Protein Immune Glass aquarium Hemocytes Granular cells Semigranular cells Hyaline cells Total plasma protein Astacus leptodactylus Crayfish Fresh waters Plasma Protein Immune Glass aquarium Samnejhad, Arash Afsharnasab, Mohammad Study of total hemocyte count and total plasma protein in immune system of freshwater Crayfish (Astacus leptodactylus) |
description |
In this study, hemocytes defence cells and total plasma protein had been studied in immune system of
freshwater Crayfish Astacus leptodactylus. About one-hundered freshwater Crayfish (A.leptodactylus)
with average weight of 25-40g were purchased from aras damreservours in west Azarbayjan province
and transported to Iranian artemia research center of orumya province.(25 pieces of Crayfish were
considered surplus for possible losses) Before experimernt the Crayfish were acclimated for two days in
the laboratory and then disinfected by oxytetracycline antibiotic and finally, an equal number (15 pieces
of Crayfish in each glass aquariums) transported to 5 glass aquariums. In order to study hemocyte and
plasma total protein, the hemolymph samples were withdrawn from abdominal second segments of
Crayfish in interval hours (0, 6, 12, 24, 48, 96, 144, 240 and 336). Result showed that the mean of
hemocytes was 27-114 CFU ml-1
, and the highest and lowest value of hemocytes were 180 and 12 CFU
ml-1
in 12 and 336 hours after experiment times, respectively. Also based on the results, the
semigranular cells(SGC) and granular cells(GC) were approximately comprised about 58-65% and 28-
37%, respectively and hyaline cells(HC) about was 3-6%. Based on total plasma protein results, the
mean of total plasma protein was 1-2 Gr dl-1
, and the highest and lowest value of total plasma protein
were 2/8 and 0/5 Gr dl-1
in 12 and 336 hours after experiment times. |
format |
Journal Contribution |
topic_facet |
Hemocytes Granular cells Semigranular cells Hyaline cells Total plasma protein Astacus leptodactylus Crayfish Fresh waters Plasma Protein Immune Glass aquarium |
author |
Samnejhad, Arash Afsharnasab, Mohammad |
author_facet |
Samnejhad, Arash Afsharnasab, Mohammad |
author_sort |
Samnejhad, Arash |
title |
Study of total hemocyte count and total plasma protein in immune system of freshwater Crayfish (Astacus leptodactylus) |
title_short |
Study of total hemocyte count and total plasma protein in immune system of freshwater Crayfish (Astacus leptodactylus) |
title_full |
Study of total hemocyte count and total plasma protein in immune system of freshwater Crayfish (Astacus leptodactylus) |
title_fullStr |
Study of total hemocyte count and total plasma protein in immune system of freshwater Crayfish (Astacus leptodactylus) |
title_full_unstemmed |
Study of total hemocyte count and total plasma protein in immune system of freshwater Crayfish (Astacus leptodactylus) |
title_sort |
study of total hemocyte count and total plasma protein in immune system of freshwater crayfish (astacus leptodactylus) |
publishDate |
2016 |
url |
http://hdl.handle.net/1834/13633 |
work_keys_str_mv |
AT samnejhadarash studyoftotalhemocytecountandtotalplasmaproteininimmunesystemoffreshwatercrayfishastacusleptodactylus AT afsharnasabmohammad studyoftotalhemocytecountandtotalplasmaproteininimmunesystemoffreshwatercrayfishastacusleptodactylus |
_version_ |
1756076610269741056 |