Studying molecular 2 species econimic shrimps (P. merguiensis, P. indicus) Northern coast of the Persian Gulf and Oman sea using microsatelitte markers to separating and identificating of their possible populations
This study focuses on molecular investigation of two commercial shrimp species of penaeus family namely as : P. merguiensis and P. indicus in order to find and introduce the genetic differentiations and also probable genotypes for monitoring and managing the genetic resources of populations in three major catch areas in the Persian Gulf and the Oman Sea. Only five out of the eight primers for p. merguiensis and four out of the eight primers for P. indicus produced good amplified PCR products with fixed annealing temperature. The rest of the primers were either not easily amplified or produced nonspecific bands. Seven and six alleles were found to be unique to each of the three populations in P.merguiensis and and two populations of P. indicus respectively. Occurrences of heterozygosity deficiency were found at most loci. These heterozygosity deficiencies in observed heterozygosity in compare to expected heterozygosity may be due to inbreeding, genetic drift and consequences of illegal overharvesting of P. merguiensis and P. indicus in the studied areas as well. Deviation from HWE in both studied species was significant in most microsatellite loci (P <0.001). We observed deviation from HWE in most loci with hetrozygosity deficits. The genetic variation results showed that the pairwise Fst values were significant between populations in both species. The assignment test for P. merguiensis revealed high gene flow between Hormoz and Jask and restricted genetic flow between Guatr and Hormoz populations .We observed high gene flow between Hormoz and Jask populations for P. indicus. It seems that the changes in immigration patterns of populations between Hormoz, Jask and Guatr areas in both species are depend on the influence of Persian Gulf currents or the life cycle of studied species. Alternatively, the presence of ecological barriers such as mangrove forests may result in restricted genetic flow between Guatr and both Hormoz and Jask populations.
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Format: | Report biblioteca |
Language: | Persian |
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Iranian Fisheries Science Research Institute
2014
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Subjects: | Commercial, Ecological, Microsatellite markers, P.merguiensis, P. indicus, PCR, Molecular, Species, Genotype, Population, Temperature, |
Online Access: | http://hdl.handle.net/1834/13399 |
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dig-aquadocs-1834-133992021-05-19T06:52:32Z Studying molecular 2 species econimic shrimps (P. merguiensis, P. indicus) Northern coast of the Persian Gulf and Oman sea using microsatelitte markers to separating and identificating of their possible populations Tamadoni Jahromi, Saeid Ghadirnezhad, S.H. Seyd Ali Babaei, S.A. Ghorghi, A. Amirinia, S. Ezhdehakosh, A. Salehi, H. Sadeghi, M.R. Commercial Ecological Microsatellite markers P.merguiensis P. indicus PCR Molecular Species Genotype Population Temperature This study focuses on molecular investigation of two commercial shrimp species of penaeus family namely as : P. merguiensis and P. indicus in order to find and introduce the genetic differentiations and also probable genotypes for monitoring and managing the genetic resources of populations in three major catch areas in the Persian Gulf and the Oman Sea. Only five out of the eight primers for p. merguiensis and four out of the eight primers for P. indicus produced good amplified PCR products with fixed annealing temperature. The rest of the primers were either not easily amplified or produced nonspecific bands. Seven and six alleles were found to be unique to each of the three populations in P.merguiensis and and two populations of P. indicus respectively. Occurrences of heterozygosity deficiency were found at most loci. These heterozygosity deficiencies in observed heterozygosity in compare to expected heterozygosity may be due to inbreeding, genetic drift and consequences of illegal overharvesting of P. merguiensis and P. indicus in the studied areas as well. Deviation from HWE in both studied species was significant in most microsatellite loci (P <0.001). We observed deviation from HWE in most loci with hetrozygosity deficits. The genetic variation results showed that the pairwise Fst values were significant between populations in both species. The assignment test for P. merguiensis revealed high gene flow between Hormoz and Jask and restricted genetic flow between Guatr and Hormoz populations .We observed high gene flow between Hormoz and Jask populations for P. indicus. It seems that the changes in immigration patterns of populations between Hormoz, Jask and Guatr areas in both species are depend on the influence of Persian Gulf currents or the life cycle of studied species. Alternatively, the presence of ecological barriers such as mangrove forests may result in restricted genetic flow between Guatr and both Hormoz and Jask populations. Iranian Fisheries Science Research Institute Published 2018-08-01T04:47:22Z 2018-08-01T04:47:22Z 2014 Report Refereed http://hdl.handle.net/1834/13399 fa 43086; 84pp. Iran Persian Gulf Oman Sea Hormoz Jask Guatr Iranian Fisheries Science Research Institute Tehran, Iran |
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Commercial Ecological Microsatellite markers P.merguiensis P. indicus PCR Molecular Species Genotype Population Temperature Commercial Ecological Microsatellite markers P.merguiensis P. indicus PCR Molecular Species Genotype Population Temperature |
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Commercial Ecological Microsatellite markers P.merguiensis P. indicus PCR Molecular Species Genotype Population Temperature Commercial Ecological Microsatellite markers P.merguiensis P. indicus PCR Molecular Species Genotype Population Temperature Tamadoni Jahromi, Saeid Ghadirnezhad, S.H. Seyd Ali Babaei, S.A. Ghorghi, A. Amirinia, S. Ezhdehakosh, A. Salehi, H. Sadeghi, M.R. Studying molecular 2 species econimic shrimps (P. merguiensis, P. indicus) Northern coast of the Persian Gulf and Oman sea using microsatelitte markers to separating and identificating of their possible populations |
description |
This study focuses on molecular investigation of two commercial shrimp species of penaeus family namely as : P. merguiensis and P. indicus in order to find and introduce the genetic differentiations and also probable genotypes for monitoring and managing the genetic resources of populations in three major catch areas in the Persian Gulf and the Oman Sea. Only five out of the eight primers for p. merguiensis and four out of the eight primers for P. indicus produced good amplified PCR products with fixed annealing temperature. The rest of the primers were either not easily amplified or produced nonspecific bands. Seven and six alleles were found to be unique to each of the three populations in P.merguiensis and and two populations of P. indicus respectively. Occurrences of heterozygosity deficiency were found at most loci. These heterozygosity deficiencies in observed heterozygosity in compare to expected heterozygosity may be due to inbreeding, genetic drift and consequences of illegal overharvesting of P. merguiensis and P. indicus in the studied areas as well. Deviation from HWE in both studied species was significant in most microsatellite loci (P <0.001). We observed deviation from HWE in most loci with hetrozygosity deficits. The genetic variation results showed that the pairwise Fst values were significant between populations in both species. The assignment test for P. merguiensis revealed high gene flow between Hormoz and Jask and restricted genetic flow between Guatr and Hormoz populations .We observed high gene flow between Hormoz and Jask populations for P. indicus. It seems that the changes in immigration patterns of populations between Hormoz, Jask and Guatr areas in both species are depend on the influence of Persian Gulf currents or the life cycle of studied species. Alternatively, the presence of ecological barriers such as mangrove forests may result in restricted genetic flow between Guatr and both Hormoz and Jask populations. |
format |
Report |
topic_facet |
Commercial Ecological Microsatellite markers P.merguiensis P. indicus PCR Molecular Species Genotype Population Temperature |
author |
Tamadoni Jahromi, Saeid Ghadirnezhad, S.H. Seyd Ali Babaei, S.A. Ghorghi, A. Amirinia, S. Ezhdehakosh, A. Salehi, H. Sadeghi, M.R. |
author_facet |
Tamadoni Jahromi, Saeid Ghadirnezhad, S.H. Seyd Ali Babaei, S.A. Ghorghi, A. Amirinia, S. Ezhdehakosh, A. Salehi, H. Sadeghi, M.R. |
author_sort |
Tamadoni Jahromi, Saeid |
title |
Studying molecular 2 species econimic shrimps (P. merguiensis, P. indicus) Northern coast of the Persian Gulf and Oman sea using microsatelitte markers to separating and identificating of their possible populations |
title_short |
Studying molecular 2 species econimic shrimps (P. merguiensis, P. indicus) Northern coast of the Persian Gulf and Oman sea using microsatelitte markers to separating and identificating of their possible populations |
title_full |
Studying molecular 2 species econimic shrimps (P. merguiensis, P. indicus) Northern coast of the Persian Gulf and Oman sea using microsatelitte markers to separating and identificating of their possible populations |
title_fullStr |
Studying molecular 2 species econimic shrimps (P. merguiensis, P. indicus) Northern coast of the Persian Gulf and Oman sea using microsatelitte markers to separating and identificating of their possible populations |
title_full_unstemmed |
Studying molecular 2 species econimic shrimps (P. merguiensis, P. indicus) Northern coast of the Persian Gulf and Oman sea using microsatelitte markers to separating and identificating of their possible populations |
title_sort |
studying molecular 2 species econimic shrimps (p. merguiensis, p. indicus) northern coast of the persian gulf and oman sea using microsatelitte markers to separating and identificating of their possible populations |
publisher |
Iranian Fisheries Science Research Institute |
publishDate |
2014 |
url |
http://hdl.handle.net/1834/13399 |
work_keys_str_mv |
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