Study of genetic structure of Rutilus frisii kutum in Golestan province coastal waters using microsatellite markers
To determine the genetic diversity of Rutilus frisii kutum, 25 samples were collected from Gharesou and Gorgan-rud rivers in April 2008. The genomic DNA was extracted from the caudal fin using phenol- chloroform method and investigated with 10 microsatellite loci. All the 10 investigated loci were polymorphic and showed variability in the two populations of the rivers. The mean number of allele per locus at population level and the observed heterozygosity ranged from 4 to 13 and 0.22 to 1.00, respectively. The FST value between populations was 0.014 (P<0.05), and the analysis of genetic variation distribution indicated a very high within population variation (99%) while among population variation is low (1%). Highly significant deviation from hardy-Weinberg equilibrium was observed in both populations which can be attributed either to the presence of null alleles or to the genetic bottleneck. The results indicated a high level of genetic variation in this species and we concluded that there is more than one population of Rutilus frisii kutum in Golestan coastal waters.
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Format: | Journal Contribution biblioteca |
Language: | Persian |
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2010
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Subjects: | Fins, Rutilus frisii kutum, Brackish, Variability, DNA, Genetic diversity, Biopolymorphism, Population number, |
Online Access: | http://hdl.handle.net/1834/11683 |
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dig-aquadocs-1834-116832021-05-19T06:45:53Z Study of genetic structure of Rutilus frisii kutum in Golestan province coastal waters using microsatellite markers Rezaei, M. Shabani, A. Shabanpour, B. Kashiri, H. Fins Rutilus frisii kutum Brackish Variability DNA Genetic diversity Biopolymorphism Population number To determine the genetic diversity of Rutilus frisii kutum, 25 samples were collected from Gharesou and Gorgan-rud rivers in April 2008. The genomic DNA was extracted from the caudal fin using phenol- chloroform method and investigated with 10 microsatellite loci. All the 10 investigated loci were polymorphic and showed variability in the two populations of the rivers. The mean number of allele per locus at population level and the observed heterozygosity ranged from 4 to 13 and 0.22 to 1.00, respectively. The FST value between populations was 0.014 (P<0.05), and the analysis of genetic variation distribution indicated a very high within population variation (99%) while among population variation is low (1%). Highly significant deviation from hardy-Weinberg equilibrium was observed in both populations which can be attributed either to the presence of null alleles or to the genetic bottleneck. The results indicated a high level of genetic variation in this species and we concluded that there is more than one population of Rutilus frisii kutum in Golestan coastal waters. Published 2018-02-24T11:50:33Z 2018-02-24T11:50:33Z 2010 Journal Contribution Refereed 1026-1354 http://hdl.handle.net/1834/11683 fa http://isfj.areo.ir/ pp.151-156 Iran Caspian Sea |
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Fins Rutilus frisii kutum Brackish Variability DNA Genetic diversity Biopolymorphism Population number Fins Rutilus frisii kutum Brackish Variability DNA Genetic diversity Biopolymorphism Population number |
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Fins Rutilus frisii kutum Brackish Variability DNA Genetic diversity Biopolymorphism Population number Fins Rutilus frisii kutum Brackish Variability DNA Genetic diversity Biopolymorphism Population number Rezaei, M. Shabani, A. Shabanpour, B. Kashiri, H. Study of genetic structure of Rutilus frisii kutum in Golestan province coastal waters using microsatellite markers |
description |
To determine the genetic diversity of Rutilus frisii kutum, 25 samples were collected from Gharesou and Gorgan-rud rivers in April 2008. The genomic DNA was extracted from the caudal fin using phenol- chloroform method and investigated with 10 microsatellite loci. All the 10 investigated loci were polymorphic and showed variability in the two populations of the rivers. The mean number of allele per locus at population level and the observed heterozygosity ranged from 4 to 13 and 0.22 to 1.00, respectively. The FST value between populations was 0.014 (P<0.05), and the analysis of genetic variation distribution indicated a very high within population variation (99%) while among population variation is low (1%). Highly significant deviation from hardy-Weinberg equilibrium was observed in both populations which can be attributed either to the presence of null alleles or to the genetic bottleneck. The results indicated a high level of genetic variation in this species and we concluded that there is more than one population of Rutilus frisii kutum in Golestan coastal waters. |
format |
Journal Contribution |
topic_facet |
Fins Rutilus frisii kutum Brackish Variability DNA Genetic diversity Biopolymorphism Population number |
author |
Rezaei, M. Shabani, A. Shabanpour, B. Kashiri, H. |
author_facet |
Rezaei, M. Shabani, A. Shabanpour, B. Kashiri, H. |
author_sort |
Rezaei, M. |
title |
Study of genetic structure of Rutilus frisii kutum in Golestan province coastal waters using microsatellite markers |
title_short |
Study of genetic structure of Rutilus frisii kutum in Golestan province coastal waters using microsatellite markers |
title_full |
Study of genetic structure of Rutilus frisii kutum in Golestan province coastal waters using microsatellite markers |
title_fullStr |
Study of genetic structure of Rutilus frisii kutum in Golestan province coastal waters using microsatellite markers |
title_full_unstemmed |
Study of genetic structure of Rutilus frisii kutum in Golestan province coastal waters using microsatellite markers |
title_sort |
study of genetic structure of rutilus frisii kutum in golestan province coastal waters using microsatellite markers |
publishDate |
2010 |
url |
http://hdl.handle.net/1834/11683 |
work_keys_str_mv |
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1756076327508639744 |