Analysis of saline activator solution effects on sperm quality indices of Barbus sharpeyi by image J software

Regarding low fertilization rate of Barbus sharpeyi, one of the main Barbus species in south of Iran, saline activator solutions (SAS 1 and 2) were used for increasing of sperm activation and its sperm quality indices were assessed by using Image J software as a fish sperm adapted tools. SAS 1 (45mM NaCl, 5mM KCl, 30mM Tris, 188±2.3 mosmol/kg) and SAS 2 (50mM NaCl, 30mM KCl, 30mM Tris, 189±1.7 mosmol.kg) were added to a mixture of 12 male semen comparing with distilled and tap water and their effects on VCL, VSL, ALH, Linearity, %motility, spermatozoa path, % A, B and C spermatozoa type, fertilization, hatching and deformity rate were analyzed in triplicate. Results showed that SAS2 had the highest VCL (21.37±0.36 µm/s-1), VSL (11.6±0.3 µm/s-1) and motility percentage (85.75±1.56 %) among treatments. The highest A type spermatozoa percentage was recorded for SAS1 (47.16±3.15 %) and SAS2 (43.89±4.54%). The lowest C type spermatozoa percentage was recorded using SAS2 (14.24±1.56 %). Also, the highest fertilization rate was achieved from SAS2 (79.5±0.37%) and SAS1 (77.74±4%) respectively. Furthermore the lowest larval deformity rate (13.77±1.03%) was shown due to the use of SAS2. Final conclusion confirmed that SAS1 and SAS2 have positive and significant effects (P < 0.05) on extending the time length of sperms movement, VCL and VSL of B. sharpeyi which consequently resulted in better efficiency of propagation of this species in south of Iran.

Bibliographic Details

Main Authors:	Kalbassi, M. R., Lorestani, R., Ghafle Maramazi, J.
Format:	Journal Contribution biblioteca
Language:	English
Published:	2013
Subjects:	Aquaculture, Software, Sperm, Saline activator solution, Fertilization, Barbus sharpeyi, Quality, Species,
Online Access:	http://hdl.handle.net/1834/11638
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