Some effects of experimental acidification on phenoloxidase, trypsin and lysozyme activities in freshwater crayfish (Pontastacus leptodactylus)
Levels of phenoloxidase (Po), trypsin and lysozyme activities were determined following 48 hours exposing freshwater crayfish (Pontastacus leptodactylus) weighing 59.8±3.4g to different low pH values of 6.5, 5.5, 4 and 2.5 at 20-23˚C. Significant decrease were found (p<0.01) in levels of Po and trypsin activities in crayfish haemocyte lysate supernatant (HLS) at pHs 5.5 and 4 compared to the control group (pH 8). Also, plasma lysozyme activity showed a significant increase in water with pHs of 6.5, 5.5 and 4 (p<0.01). Furthermore, measurement of calcium and magnesium concentrations in sera samples showed a significant decrease for calcium ion at pHs 5.5 and 4, while magnesium concentration was significantly decreased only at pH 4 compared to control group (p<0.05). Crayfish exposed to pH 2.5 died within 24 hours post-exposure.
Main Authors: | , , |
---|---|
Format: | Journal Contribution biblioteca |
Language: | English |
Published: |
2008
|
Subjects: | Crayfish, Pontastacus leptodactylus, Trypsin, Serum calcium and magnesium, Lysozyme, Phenoloxidase, |
Online Access: | http://hdl.handle.net/1834/11188 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-aquadocs-1834-11188 |
---|---|
record_format |
koha |
spelling |
dig-aquadocs-1834-111882021-05-19T06:43:33Z Some effects of experimental acidification on phenoloxidase, trypsin and lysozyme activities in freshwater crayfish (Pontastacus leptodactylus) Soltani, M. Khazraeenia, S. Sepehri, H. Crayfish Pontastacus leptodactylus Trypsin Serum calcium and magnesium Lysozyme Phenoloxidase Levels of phenoloxidase (Po), trypsin and lysozyme activities were determined following 48 hours exposing freshwater crayfish (Pontastacus leptodactylus) weighing 59.8±3.4g to different low pH values of 6.5, 5.5, 4 and 2.5 at 20-23˚C. Significant decrease were found (p<0.01) in levels of Po and trypsin activities in crayfish haemocyte lysate supernatant (HLS) at pHs 5.5 and 4 compared to the control group (pH 8). Also, plasma lysozyme activity showed a significant increase in water with pHs of 6.5, 5.5 and 4 (p<0.01). Furthermore, measurement of calcium and magnesium concentrations in sera samples showed a significant decrease for calcium ion at pHs 5.5 and 4, while magnesium concentration was significantly decreased only at pH 4 compared to control group (p<0.05). Crayfish exposed to pH 2.5 died within 24 hours post-exposure. Published 2018-02-10T12:57:00Z 2018-02-10T12:57:00Z 2008 Journal Contribution Refereed 1562-2916 http://hdl.handle.net/1834/11188 en pp.87-102 Iran Tehran |
institution |
UNESCO |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-aquadocs |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Repositorio AQUADOCS |
language |
English |
topic |
Crayfish Pontastacus leptodactylus Trypsin Serum calcium and magnesium Lysozyme Phenoloxidase Crayfish Pontastacus leptodactylus Trypsin Serum calcium and magnesium Lysozyme Phenoloxidase |
spellingShingle |
Crayfish Pontastacus leptodactylus Trypsin Serum calcium and magnesium Lysozyme Phenoloxidase Crayfish Pontastacus leptodactylus Trypsin Serum calcium and magnesium Lysozyme Phenoloxidase Soltani, M. Khazraeenia, S. Sepehri, H. Some effects of experimental acidification on phenoloxidase, trypsin and lysozyme activities in freshwater crayfish (Pontastacus leptodactylus) |
description |
Levels of phenoloxidase (Po), trypsin and lysozyme activities were determined following 48 hours exposing freshwater crayfish (Pontastacus leptodactylus) weighing 59.8±3.4g to different low pH values of 6.5, 5.5, 4 and 2.5 at 20-23˚C. Significant decrease were found (p<0.01) in levels of Po and trypsin activities in crayfish haemocyte lysate supernatant (HLS) at pHs 5.5 and 4 compared to the control group (pH 8). Also, plasma lysozyme activity showed a significant increase in water with pHs of 6.5, 5.5 and 4 (p<0.01). Furthermore, measurement of calcium and magnesium concentrations in sera samples showed a significant decrease for calcium ion at pHs 5.5 and 4, while magnesium concentration was significantly decreased only at pH 4 compared to control group (p<0.05). Crayfish exposed to pH 2.5 died within 24 hours post-exposure. |
format |
Journal Contribution |
topic_facet |
Crayfish Pontastacus leptodactylus Trypsin Serum calcium and magnesium Lysozyme Phenoloxidase |
author |
Soltani, M. Khazraeenia, S. Sepehri, H. |
author_facet |
Soltani, M. Khazraeenia, S. Sepehri, H. |
author_sort |
Soltani, M. |
title |
Some effects of experimental acidification on phenoloxidase, trypsin and lysozyme activities in freshwater crayfish (Pontastacus leptodactylus) |
title_short |
Some effects of experimental acidification on phenoloxidase, trypsin and lysozyme activities in freshwater crayfish (Pontastacus leptodactylus) |
title_full |
Some effects of experimental acidification on phenoloxidase, trypsin and lysozyme activities in freshwater crayfish (Pontastacus leptodactylus) |
title_fullStr |
Some effects of experimental acidification on phenoloxidase, trypsin and lysozyme activities in freshwater crayfish (Pontastacus leptodactylus) |
title_full_unstemmed |
Some effects of experimental acidification on phenoloxidase, trypsin and lysozyme activities in freshwater crayfish (Pontastacus leptodactylus) |
title_sort |
some effects of experimental acidification on phenoloxidase, trypsin and lysozyme activities in freshwater crayfish (pontastacus leptodactylus) |
publishDate |
2008 |
url |
http://hdl.handle.net/1834/11188 |
work_keys_str_mv |
AT soltanim someeffectsofexperimentalacidificationonphenoloxidasetrypsinandlysozymeactivitiesinfreshwatercrayfishpontastacusleptodactylus AT khazraeenias someeffectsofexperimentalacidificationonphenoloxidasetrypsinandlysozymeactivitiesinfreshwatercrayfishpontastacusleptodactylus AT sepehrih someeffectsofexperimentalacidificationonphenoloxidasetrypsinandlysozymeactivitiesinfreshwatercrayfishpontastacusleptodactylus |
_version_ |
1756076204116410368 |