In vitro cytotoxic and anti-cancer effects of body wall for sea cucumber (Holothuria leucospilota)
In recent years efforts to find bioactive compounds from live organisms especially marine animals have been increased. In the present study, the anticancer and cytotoxic effects of sea cucumber body walls (Holothuria leucospilota) were investigated. For this purpose, sea cucumbers were collected from Larak Island at depths of 10 to 30 m and extraction process was done with methanol and diethyl ether solvent which then concentrated by rotary evaporator (40℃) following lyophilization with vacuum freeze dryer. XTT method was used to investigate anticancer and cytotoxic effects of body wall extracts. The results showed that the methanolic extract could prevent proliferation of human oral epidermoid carcinoma cells (KB) at concentrations of 100 and 500 μg/ml. The diethyl etheric extract also could prevent proliferation of KB at 500 μg/ml concentration. Overall result showed that sea cucumber body wall had a strong cytotoxic effect on normal cell line (Human embryonic kidney cell [HEK]) which can be used as potent cytotoxic material. However these extracts did not show significant therapeutic value against KB cells.
Main Authors: | , , , , |
---|---|
Format: | Journal Contribution biblioteca |
Language: | Persian |
Published: |
2014
|
Subjects: | In vitro, Sea cucumber, Methanolic extract, Diethyl etheric extract, Cytotoxic, Anti-cancer, Holothuria leucospilota, Body Wall, Diet, |
Online Access: | http://hdl.handle.net/1834/10749 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-aquadocs-1834-10749 |
---|---|
record_format |
koha |
spelling |
dig-aquadocs-1834-107492021-05-19T06:45:51Z In vitro cytotoxic and anti-cancer effects of body wall for sea cucumber (Holothuria leucospilota) Bahroodi, S. Nematollahi, M.A. Aghasadeghi, M.R. Nazemi, M. Behroz, B. In vitro Sea cucumber Methanolic extract Diethyl etheric extract Cytotoxic Anti-cancer Holothuria leucospilota Body Wall Diet In recent years efforts to find bioactive compounds from live organisms especially marine animals have been increased. In the present study, the anticancer and cytotoxic effects of sea cucumber body walls (Holothuria leucospilota) were investigated. For this purpose, sea cucumbers were collected from Larak Island at depths of 10 to 30 m and extraction process was done with methanol and diethyl ether solvent which then concentrated by rotary evaporator (40℃) following lyophilization with vacuum freeze dryer. XTT method was used to investigate anticancer and cytotoxic effects of body wall extracts. The results showed that the methanolic extract could prevent proliferation of human oral epidermoid carcinoma cells (KB) at concentrations of 100 and 500 μg/ml. The diethyl etheric extract also could prevent proliferation of KB at 500 μg/ml concentration. Overall result showed that sea cucumber body wall had a strong cytotoxic effect on normal cell line (Human embryonic kidney cell [HEK]) which can be used as potent cytotoxic material. However these extracts did not show significant therapeutic value against KB cells. Published 2017-12-21T14:21:53Z 2017-12-21T14:21:53Z 2014 Journal Contribution Refereed 1026-1354 http://hdl.handle.net/1834/10749 fa pp.11-20 Iran |
institution |
UNESCO |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-aquadocs |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Repositorio AQUADOCS |
language |
Persian |
topic |
In vitro Sea cucumber Methanolic extract Diethyl etheric extract Cytotoxic Anti-cancer Holothuria leucospilota Body Wall Diet In vitro Sea cucumber Methanolic extract Diethyl etheric extract Cytotoxic Anti-cancer Holothuria leucospilota Body Wall Diet |
spellingShingle |
In vitro Sea cucumber Methanolic extract Diethyl etheric extract Cytotoxic Anti-cancer Holothuria leucospilota Body Wall Diet In vitro Sea cucumber Methanolic extract Diethyl etheric extract Cytotoxic Anti-cancer Holothuria leucospilota Body Wall Diet Bahroodi, S. Nematollahi, M.A. Aghasadeghi, M.R. Nazemi, M. Behroz, B. In vitro cytotoxic and anti-cancer effects of body wall for sea cucumber (Holothuria leucospilota) |
description |
In recent years efforts to find bioactive compounds from live organisms especially marine
animals have been increased. In the present study, the anticancer and cytotoxic effects of sea
cucumber body walls (Holothuria leucospilota) were investigated. For this purpose, sea
cucumbers were collected from Larak Island at depths of 10 to 30 m and extraction process
was done with methanol and diethyl ether solvent which then concentrated by rotary
evaporator (40℃) following lyophilization with vacuum freeze dryer. XTT method was used
to investigate anticancer and cytotoxic effects of body wall extracts. The results showed that
the methanolic extract could prevent proliferation of human oral epidermoid carcinoma cells
(KB) at concentrations of 100 and 500 μg/ml. The diethyl etheric extract also could prevent
proliferation of KB at 500 μg/ml concentration. Overall result showed that sea cucumber body
wall had a strong cytotoxic effect on normal cell line (Human embryonic kidney cell [HEK])
which can be used as potent cytotoxic material. However these extracts did not show
significant therapeutic value against KB cells. |
format |
Journal Contribution |
topic_facet |
In vitro Sea cucumber Methanolic extract Diethyl etheric extract Cytotoxic Anti-cancer Holothuria leucospilota Body Wall Diet |
author |
Bahroodi, S. Nematollahi, M.A. Aghasadeghi, M.R. Nazemi, M. Behroz, B. |
author_facet |
Bahroodi, S. Nematollahi, M.A. Aghasadeghi, M.R. Nazemi, M. Behroz, B. |
author_sort |
Bahroodi, S. |
title |
In vitro cytotoxic and anti-cancer effects of body wall for sea cucumber (Holothuria leucospilota) |
title_short |
In vitro cytotoxic and anti-cancer effects of body wall for sea cucumber (Holothuria leucospilota) |
title_full |
In vitro cytotoxic and anti-cancer effects of body wall for sea cucumber (Holothuria leucospilota) |
title_fullStr |
In vitro cytotoxic and anti-cancer effects of body wall for sea cucumber (Holothuria leucospilota) |
title_full_unstemmed |
In vitro cytotoxic and anti-cancer effects of body wall for sea cucumber (Holothuria leucospilota) |
title_sort |
in vitro cytotoxic and anti-cancer effects of body wall for sea cucumber (holothuria leucospilota) |
publishDate |
2014 |
url |
http://hdl.handle.net/1834/10749 |
work_keys_str_mv |
AT bahroodis invitrocytotoxicandanticancereffectsofbodywallforseacucumberholothurialeucospilota AT nematollahima invitrocytotoxicandanticancereffectsofbodywallforseacucumberholothurialeucospilota AT aghasadeghimr invitrocytotoxicandanticancereffectsofbodywallforseacucumberholothurialeucospilota AT nazemim invitrocytotoxicandanticancereffectsofbodywallforseacucumberholothurialeucospilota AT behrozb invitrocytotoxicandanticancereffectsofbodywallforseacucumberholothurialeucospilota |
_version_ |
1756076323689725952 |