Failure of PCR-RAPD technique to differentiate sex in Mahisefied (Rutilus frisii kutum) from the South Caspian Sea
In order to identify the sex marker in Mahisefied, Rutilus frisii kutum, samples from 5 male and 5 female fish were collected from the south Caspian Sea. Polymerase chain reaction random amplified polymorphic DNA (PCR-RAPD) was performed using 124 primer sets. All bands were numbered using 1 and 0 scores corresponding to the presence or absence of bands, respectively and data were analyzed using RAPDPLOT program. Results indicated that 44 sets of primers did not show any flanking site and produced no bands, while the remaining 80 produced sharp and visible bands on polyacrylamid gel. In total, 1600 bands were scored. However, none of the bands corresponded to either the male or female fish. According to the results it has been concluded that RAPD technique failed to detect sex and cannot be considered as a robust molecular tool for sex differentiation in the studied fish. The reason may be the absence of sex chromosomes in this species or that the genes corresponding to sex differentiation are spread on different autosomal chromosomes with interaction of some environmental factors.
Main Authors: | Pourkazemi, M., Razikazemi, S. |
---|---|
Format: | Journal Contribution biblioteca |
Language: | English |
Published: |
2011
|
Subjects: | Rutilus frisii kutum, Mahisefid, Random Amplified Polymorphic DNA, Sex determination, Failure, Sex, DNA, |
Online Access: | http://hdl.handle.net/1834/10411 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Failure of PCR-RAPD technique to differentiate sex in Mahisefied (Rutilus frisii kutum) from the south Caspian Sea
by: Pourkazemi, M., et al.
Published: (2011) -
Morphometrics studies of Mahisefid (Rutilus frisii kutum, Kamensky, 1901) from selected rivers in the southern Caspian Sea
by: Abdolhay, H. A., et al.
Published: (2010) -
Genetic diversity of Mahisefid (Rutilus frisii kutum Kamensky 1901) in different rivers of the south Caspian Sea using PCR-RFLP
by: Abdolhay, H. A., et al.
Published: (2012) -
Study on sex steroid-binding proteins (with emphasize on 17 b-estradiol) in plasma of female and juvenile kutum (Rutilus frisii kutum)
by: Kousha, A., et al.
Published: (2007) -
Study of Kutum (Rutilus frisii kutum) population using mtDNA with PCR
by: Abdolhay, Hossein Ali, et al.
Published: (2011)