Failure of PCR-RAPD technique to differentiate sex in Mahisefied (Rutilus frisii kutum) from the South Caspian Sea
In order to identify the sex marker in Mahisefied, Rutilus frisii kutum, samples from 5 male and 5 female fish were collected from the south Caspian Sea. Polymerase chain reaction random amplified polymorphic DNA (PCR-RAPD) was performed using 124 primer sets. All bands were numbered using 1 and 0 scores corresponding to the presence or absence of bands, respectively and data were analyzed using RAPDPLOT program. Results indicated that 44 sets of primers did not show any flanking site and produced no bands, while the remaining 80 produced sharp and visible bands on polyacrylamid gel. In total, 1600 bands were scored. However, none of the bands corresponded to either the male or female fish. According to the results it has been concluded that RAPD technique failed to detect sex and cannot be considered as a robust molecular tool for sex differentiation in the studied fish. The reason may be the absence of sex chromosomes in this species or that the genes corresponding to sex differentiation are spread on different autosomal chromosomes with interaction of some environmental factors.
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Format: | Journal Contribution biblioteca |
Language: | English |
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2011
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Subjects: | Rutilus frisii kutum, Mahisefid, Random Amplified Polymorphic DNA, Sex determination, Failure, Sex, DNA, |
Online Access: | http://hdl.handle.net/1834/10411 |
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dig-aquadocs-1834-104112021-05-19T06:54:10Z Failure of PCR-RAPD technique to differentiate sex in Mahisefied (Rutilus frisii kutum) from the South Caspian Sea Pourkazemi, M. Razikazemi, S. Rutilus frisii kutum Mahisefid Random Amplified Polymorphic DNA Sex determination Failure Sex DNA In order to identify the sex marker in Mahisefied, Rutilus frisii kutum, samples from 5 male and 5 female fish were collected from the south Caspian Sea. Polymerase chain reaction random amplified polymorphic DNA (PCR-RAPD) was performed using 124 primer sets. All bands were numbered using 1 and 0 scores corresponding to the presence or absence of bands, respectively and data were analyzed using RAPDPLOT program. Results indicated that 44 sets of primers did not show any flanking site and produced no bands, while the remaining 80 produced sharp and visible bands on polyacrylamid gel. In total, 1600 bands were scored. However, none of the bands corresponded to either the male or female fish. According to the results it has been concluded that RAPD technique failed to detect sex and cannot be considered as a robust molecular tool for sex differentiation in the studied fish. The reason may be the absence of sex chromosomes in this species or that the genes corresponding to sex differentiation are spread on different autosomal chromosomes with interaction of some environmental factors. Published 2017-11-17T07:25:13Z 2017-11-17T07:25:13Z 2011 Journal Contribution Refereed 1735-3033 http://hdl.handle.net/1834/10411 en pp.235-242 Iran Caspian Sea |
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Rutilus frisii kutum Mahisefid Random Amplified Polymorphic DNA Sex determination Failure Sex DNA Rutilus frisii kutum Mahisefid Random Amplified Polymorphic DNA Sex determination Failure Sex DNA |
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Rutilus frisii kutum Mahisefid Random Amplified Polymorphic DNA Sex determination Failure Sex DNA Rutilus frisii kutum Mahisefid Random Amplified Polymorphic DNA Sex determination Failure Sex DNA Pourkazemi, M. Razikazemi, S. Failure of PCR-RAPD technique to differentiate sex in Mahisefied (Rutilus frisii kutum) from the South Caspian Sea |
description |
In order to identify the sex marker in Mahisefied, Rutilus frisii kutum, samples from 5 male and 5 female
fish were collected from the south Caspian Sea. Polymerase chain reaction random amplified polymorphic
DNA (PCR-RAPD) was performed using 124 primer sets. All bands were numbered using 1 and 0 scores
corresponding to the presence or absence of bands, respectively and data were analyzed using
RAPDPLOT program. Results indicated that 44 sets of primers did not show any flanking site and
produced no bands, while the remaining 80 produced sharp and visible bands on polyacrylamid gel. In
total, 1600 bands were scored. However, none of the bands corresponded to either the male or female fish.
According to the results it has been concluded that RAPD technique failed to detect sex and cannot be
considered as a robust molecular tool for sex differentiation in the studied fish. The reason may be the
absence of sex chromosomes in this species or that the genes corresponding to sex differentiation are
spread on different autosomal chromosomes with interaction of some environmental factors. |
format |
Journal Contribution |
topic_facet |
Rutilus frisii kutum Mahisefid Random Amplified Polymorphic DNA Sex determination Failure Sex DNA |
author |
Pourkazemi, M. Razikazemi, S. |
author_facet |
Pourkazemi, M. Razikazemi, S. |
author_sort |
Pourkazemi, M. |
title |
Failure of PCR-RAPD technique to differentiate sex in Mahisefied (Rutilus frisii kutum) from the South Caspian Sea |
title_short |
Failure of PCR-RAPD technique to differentiate sex in Mahisefied (Rutilus frisii kutum) from the South Caspian Sea |
title_full |
Failure of PCR-RAPD technique to differentiate sex in Mahisefied (Rutilus frisii kutum) from the South Caspian Sea |
title_fullStr |
Failure of PCR-RAPD technique to differentiate sex in Mahisefied (Rutilus frisii kutum) from the South Caspian Sea |
title_full_unstemmed |
Failure of PCR-RAPD technique to differentiate sex in Mahisefied (Rutilus frisii kutum) from the South Caspian Sea |
title_sort |
failure of pcr-rapd technique to differentiate sex in mahisefied (rutilus frisii kutum) from the south caspian sea |
publishDate |
2011 |
url |
http://hdl.handle.net/1834/10411 |
work_keys_str_mv |
AT pourkazemim failureofpcrrapdtechniquetodifferentiatesexinmahisefiedrutilusfrisiikutumfromthesouthcaspiansea AT razikazemis failureofpcrrapdtechniquetodifferentiatesexinmahisefiedrutilusfrisiikutumfromthesouthcaspiansea |
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