Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe.
The viruses Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV) and Apple mosaic virus (ApMV) are common in apples and pears and main targets of detection in propagation materials. This study aimed at demonstrating the usefulness of the hybridization method with a non-radioactive probe for simultaneous detection of these four viruses. The sensitivity of this method was suffi ciently high enabling the detection of ASGV, ACLSV, ASPV and ApMV in total RNA extracted from infected samples. The probe specifi city was confi rmed by reaction with homologous viral cDNA, individually cloned for each virus.
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Format: | Artigo de periódico biblioteca |
Language: | English eng |
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2014-11-03
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Subjects: | ASGV, ACLSV, ASPV, ApMV, Macieira, Polissonda., Maçã, Doença de planta, Hibridação, Apple stem grooving virus, Apple stem pitting virus., Apple chlorotic leaf spot virus, Apple mosaic virus, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/999019 |
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dig-alice-doc-9990192017-08-16T00:20:13Z Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe. FAJARDO, T. V. M. NICKEL, O. THOR VINICIUS MARTINS FAJARDO, CNPUV; OSMAR NICKEL, CNPUV. ASGV ACLSV ASPV ApMV Macieira Polissonda. Maçã Doença de planta Hibridação Apple stem grooving virus Apple stem pitting virus. Apple chlorotic leaf spot virus Apple mosaic virus The viruses Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV) and Apple mosaic virus (ApMV) are common in apples and pears and main targets of detection in propagation materials. This study aimed at demonstrating the usefulness of the hybridization method with a non-radioactive probe for simultaneous detection of these four viruses. The sensitivity of this method was suffi ciently high enabling the detection of ASGV, ACLSV, ASPV and ApMV in total RNA extracted from infected samples. The probe specifi city was confi rmed by reaction with homologous viral cDNA, individually cloned for each virus. 2014-11-03T11:11:11Z 2014-11-03T11:11:11Z 2014-11-03 2014 2014-11-03T11:11:11Z Artigo de periódico Ciência Rural, Santa Maria, v. 44, n. 10, p. 1711-1714, out. 2014. http://www.alice.cnptia.embrapa.br/alice/handle/doc/999019 en eng openAccess |
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ASGV ACLSV ASPV ApMV Macieira Polissonda. Maçã Doença de planta Hibridação Apple stem grooving virus Apple stem pitting virus. Apple chlorotic leaf spot virus Apple mosaic virus ASGV ACLSV ASPV ApMV Macieira Polissonda. Maçã Doença de planta Hibridação Apple stem grooving virus Apple stem pitting virus. Apple chlorotic leaf spot virus Apple mosaic virus |
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ASGV ACLSV ASPV ApMV Macieira Polissonda. Maçã Doença de planta Hibridação Apple stem grooving virus Apple stem pitting virus. Apple chlorotic leaf spot virus Apple mosaic virus ASGV ACLSV ASPV ApMV Macieira Polissonda. Maçã Doença de planta Hibridação Apple stem grooving virus Apple stem pitting virus. Apple chlorotic leaf spot virus Apple mosaic virus FAJARDO, T. V. M. NICKEL, O. Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe. |
description |
The viruses Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV) and Apple mosaic virus (ApMV) are common in apples and pears and main targets of detection in propagation materials. This study aimed at demonstrating the usefulness of the hybridization method with a non-radioactive probe for simultaneous detection of these four viruses. The sensitivity of this method was suffi ciently high enabling the detection of ASGV, ACLSV, ASPV and ApMV in total RNA extracted from infected samples. The probe specifi city was confi rmed by reaction with homologous viral cDNA, individually cloned for each virus. |
author2 |
THOR VINICIUS MARTINS FAJARDO, CNPUV; OSMAR NICKEL, CNPUV. |
author_facet |
THOR VINICIUS MARTINS FAJARDO, CNPUV; OSMAR NICKEL, CNPUV. FAJARDO, T. V. M. NICKEL, O. |
format |
Artigo de periódico |
topic_facet |
ASGV ACLSV ASPV ApMV Macieira Polissonda. Maçã Doença de planta Hibridação Apple stem grooving virus Apple stem pitting virus. Apple chlorotic leaf spot virus Apple mosaic virus |
author |
FAJARDO, T. V. M. NICKEL, O. |
author_sort |
FAJARDO, T. V. M. |
title |
Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe. |
title_short |
Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe. |
title_full |
Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe. |
title_fullStr |
Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe. |
title_full_unstemmed |
Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe. |
title_sort |
simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe. |
publishDate |
2014-11-03 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/999019 |
work_keys_str_mv |
AT fajardotvm simultaneousdetectionoffourvirusesaffectingappleandpearbymolecularhybridizationusingapolyprobe AT nickelo simultaneousdetectionoffourvirusesaffectingappleandpearbymolecularhybridizationusingapolyprobe |
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1756020008594440192 |