Rooting and acclimatization of micropropagated marubakaido apple rootstock using Adesmia latifolia rhizobia.
In vitro rooting and the acclimatization of micropropagated rootstocks of apple trees is essential for plant development in the field. The aim of this work was to assess the use of rhizobia of Adesmia latifolia to promote rooting and acclimatization in micropropagated Marubakaido apple rootstock. An experiment involving in vitro rooting and acclimatization was performed with four strains of rhizobium and two controls, one with and the other without the addition of synthetic indoleacetic acid. The inoculated treatments involved the use of sterile inoculum and inoculum containing live rhizobia. The most significant effects on the rooting rate, primary-root length, number of roots, root length, fresh-shoot biomass, and fresh-root biomass were obtained by inoculation with strain EEL16010B and with synthetic indole acetic acid. However, there was no difference in the growth of apple explants in the acclimatization experiments. Strain EEL16010B can be used to induce in vitro rooting of the Marubakaido rootstock and can replace the use of synthetic indoleacetic acid in the rooting of this cultivar.
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2013-09-10
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Subjects: | Apple, Indoleacetic acid., Maçã, Micropropagação., auxins, micropropagation., |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/965953 |
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dig-alice-doc-9659532017-08-16T00:59:28Z Rooting and acclimatization of micropropagated marubakaido apple rootstock using Adesmia latifolia rhizobia. MUNIZ, A. W. SÁ, E. L. de DALAGNOL, G. L. AMÉRICO FILHO, J. ALEKSANDER WESTPHAL MUNIZ, CPAA; Enilson Luiz de Sá, UFRGS; Gilberto Luíz Dalagnol, EPAGRI; João Américo Filho, EPAGRI. Apple Indoleacetic acid. Maçã Micropropagação. auxins micropropagation. In vitro rooting and the acclimatization of micropropagated rootstocks of apple trees is essential for plant development in the field. The aim of this work was to assess the use of rhizobia of Adesmia latifolia to promote rooting and acclimatization in micropropagated Marubakaido apple rootstock. An experiment involving in vitro rooting and acclimatization was performed with four strains of rhizobium and two controls, one with and the other without the addition of synthetic indoleacetic acid. The inoculated treatments involved the use of sterile inoculum and inoculum containing live rhizobia. The most significant effects on the rooting rate, primary-root length, number of roots, root length, fresh-shoot biomass, and fresh-root biomass were obtained by inoculation with strain EEL16010B and with synthetic indole acetic acid. However, there was no difference in the growth of apple explants in the acclimatization experiments. Strain EEL16010B can be used to induce in vitro rooting of the Marubakaido rootstock and can replace the use of synthetic indoleacetic acid in the rooting of this cultivar. 2013-09-10T11:11:11Z 2013-09-10T11:11:11Z 2013-09-10 2013 2013-09-17T11:11:11Z Separatas SpringerPlus, v. 2, p. 437, 2013. 2193-1801 http://www.alice.cnptia.embrapa.br/alice/handle/doc/965953 10.1186/2193-1801-2-437 pt_BR por openAccess |
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Apple Indoleacetic acid. Maçã Micropropagação. auxins micropropagation. Apple Indoleacetic acid. Maçã Micropropagação. auxins micropropagation. |
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Apple Indoleacetic acid. Maçã Micropropagação. auxins micropropagation. Apple Indoleacetic acid. Maçã Micropropagação. auxins micropropagation. MUNIZ, A. W. SÁ, E. L. de DALAGNOL, G. L. AMÉRICO FILHO, J. Rooting and acclimatization of micropropagated marubakaido apple rootstock using Adesmia latifolia rhizobia. |
description |
In vitro rooting and the acclimatization of micropropagated rootstocks of apple trees is essential for plant development in the field. The aim of this work was to assess the use of rhizobia of Adesmia latifolia to promote rooting and acclimatization in micropropagated Marubakaido apple rootstock. An experiment involving in vitro rooting and acclimatization was performed with four strains of rhizobium and two controls, one with and the other without the addition of synthetic indoleacetic acid. The inoculated treatments involved the use of sterile inoculum and inoculum containing live rhizobia. The most significant effects on the rooting rate, primary-root length, number of roots, root length, fresh-shoot biomass, and fresh-root biomass were obtained by inoculation with strain EEL16010B and with synthetic indole acetic acid. However, there was no difference in the growth of apple explants in the acclimatization experiments. Strain EEL16010B can be used to induce in vitro rooting of the Marubakaido rootstock and can replace the use of synthetic indoleacetic acid in the rooting of this cultivar. |
author2 |
ALEKSANDER WESTPHAL MUNIZ, CPAA; Enilson Luiz de Sá, UFRGS; Gilberto Luíz Dalagnol, EPAGRI; João Américo Filho, EPAGRI. |
author_facet |
ALEKSANDER WESTPHAL MUNIZ, CPAA; Enilson Luiz de Sá, UFRGS; Gilberto Luíz Dalagnol, EPAGRI; João Américo Filho, EPAGRI. MUNIZ, A. W. SÁ, E. L. de DALAGNOL, G. L. AMÉRICO FILHO, J. |
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Separatas |
topic_facet |
Apple Indoleacetic acid. Maçã Micropropagação. auxins micropropagation. |
author |
MUNIZ, A. W. SÁ, E. L. de DALAGNOL, G. L. AMÉRICO FILHO, J. |
author_sort |
MUNIZ, A. W. |
title |
Rooting and acclimatization of micropropagated marubakaido apple rootstock using Adesmia latifolia rhizobia. |
title_short |
Rooting and acclimatization of micropropagated marubakaido apple rootstock using Adesmia latifolia rhizobia. |
title_full |
Rooting and acclimatization of micropropagated marubakaido apple rootstock using Adesmia latifolia rhizobia. |
title_fullStr |
Rooting and acclimatization of micropropagated marubakaido apple rootstock using Adesmia latifolia rhizobia. |
title_full_unstemmed |
Rooting and acclimatization of micropropagated marubakaido apple rootstock using Adesmia latifolia rhizobia. |
title_sort |
rooting and acclimatization of micropropagated marubakaido apple rootstock using adesmia latifolia rhizobia. |
publishDate |
2013-09-10 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/965953 |
work_keys_str_mv |
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