Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit.

Soybean has a wide range of applications in the industry and, due to its crop potential, its improvement is widely desirable. During drought conditions, soybean crops suffer significant losses in productivity. Therefore, understanding the responses of the soybean under this stress is an effective way of targeting crop improvement techniques. In this study, we employed the Suppressive Subtractive Hybridization (SSH) technique to investigate differentially expressed genes under water deficit conditions. Embrapa 48 and BR 16 soybean lines, known as drought-tolerant and -sensitive, respectively, were grown hydroponically and subjected to different short-term periods of stress by withholding the nutrient solution. Using this approach, we have identified genes expressed during the early response to water deficit in roots and leaves. These genes were compared among the lines to assess probable differences in the plant transcriptomes. In general, similar biochemical processes were predominant in both cultivars; however, there were more considerable differences between roots and leaves of Embrapa 48. Moreover, we present here a fast, clean and straightforward method to obtain drought-stressed root tissues and a large enriched collection of transcripts expressed by soybean plants under water deficit that can be useful for further studies towards the understanding of plant responses to stress.

Bibliographic Details

Main Authors:	RODRIGUES, F. A., MARCOLINO-GOMES, J., CARVALHO, J. de F. C., NASCIMENTO, L. C. do, NEUMAIER, N., FARIAS, J. R. B., CARAZZOLLE, M. F., MARCELINO, F. C., NEPOMUCENO, A. L.
Other Authors:	FABIANA APARECIDA RODRIGUES, CNPSo; JULIANA MARCOLINO-GOMES, CNPSo; JOSIRLEI DE FÁTIMA CORRÊA CARVALHO, CARVALHO , J. de F. C.; LEANDRO COSTA DO NASCIMENTO, UNICAMP; NORMAN NEUMAIER, CNPSO; JOSE RENATO BOUCAS FARIAS, CNPSO; MARCELO FALSARELLA CARAZZOLLE, UNICAMP; FRANCISMAR CORREA MARCELINO GUIMARÃES, CNPSO; ALEXANDRE LIMA NEPOMUCENO, SRI.
Format:	Artigo de periódico biblioteca
Language:	English eng
Published:	2012-06-18
Subjects:	Soja, Genoma, Gene, Deficiência hídrica, Relação água-planta, Seca, Estiagem, Soybeans, Gene expression, Drought,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/926619
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