Differential expression of genes during mastitis in Holstein-Zebu crossbreed dairy cows.

Among the potential public health problems of animal production, infectious-contagious diseases stand out. Mastitis is among the main diseases affecting dairy cattle. One of the most promising options to reduce the problems caused by this disease, besides proper sanitary and management practices, is selective breeding of resistant animals. To shed light on the immune response mechanisms involved in the resistance/susceptibility phenotype to this disease, we quantified the relative expression of the genes IL-2, IL-6, IL-8, IL-12, IFN-γ, TNF-α, TLR-2, SEMA5A, and FEZL in cells of crossbreed dairy cows, divided into two groups, one healthy and the other suffering from clinical mastitis. Total RNA was extracted from the cells in the milk from the animals in each group (with and without clinical mastitis). Gene expression was determined using the real-time PCR method. The levels of gene expression were compared, and the cows with mastitis were found to express 2.5 times more TLR-2 than those free of mastitis (P < 0.05). There were no significant differences in the expression of the other genes.

Bibliographic Details

Main Authors:	FONSECA, I., ANTUNES, G. R., PAIVA, D. de S., LANGE, C. C., GUIMARÃES, S. E. F., MARTINS, M. F.
Other Authors:	ISABELA FONSECA, UFV; GUSTAVO RESENDE ANTUNES, UFJF; DAISYLÉA DE SOUZA PAIVA, UFJF; CARLA CHRISTINE LANGE, CNPGL; SIMONE ELISA FACIONI GUIMARÃES, UFV; MARTA FONSECA MARTINS, CNPGL.
Format:	Artigo de periódico biblioteca
Language:	Ingles English
Published:	2011-10-04
Subjects:	Real-time PCR, Resistance to mastitis, Toll-like receptor 2 gene, immune response,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/902309 https://doi.org/10.4238/vol10-3gmr1096
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