Coffee seed cryopreservation: current research progress.
Viability of seeds stored in genebanks must be maintained for several years or even centuries. Because of the difficulties in storing the seeds, coffee germplasm is maintained in field collections, presenting significant problems, such as land and labor costs and susceptibility to environmental hazards and pathogens. Storage of Coffea species in ex situ genebanks may help to preserve the threatened diversity of this important genus. Since 1976 the International Plant Genetic Resources Institute has considered coffee as a high priority for genetic conservation. For non-orthodox seed species, cryopreservation is the only technique available for long-term germplasm conservation. The genebank of Embrapa Genetic Resources and Biotechnology, in Brazil has now established a program to cryopreserve genetic resources of Coffea. The protocol was first determined for C. arabica and C. racemosa. Seeds were first dried to 0.20 g/g (in equilibrium with 78-80% RH). Sufficiently rapid cooling and warming was achieved in hermetically-sealed foil-laminate bags containing 10-11 g (or 50 seeds) by plunging bags directly into liquid nitrogen ? LN containers and placing bags removed from LN directly into a 40 °C bath. Successfully cryopreserved C. arabica and C. racemosa seeds showed minimal viability loss after two-year storage in liquid nitrogen. The same protocol is being adapted to other species of Coffea.
Main Authors: | EIRA, M. T. S. da, RIBEIRO, V. S., FUZUOLI, L. C., GUERREIRO FILHO, O., WALTERS, C. |
---|---|
Other Authors: | MIRIAN THEREZINHA SOUZA DA EIRA, SAPC; IAC; IAC; NCGRP/USDA. |
Format: | Anais e Proceedings de eventos biblioteca |
Language: | English eng |
Published: |
2011-04-13
|
Subjects: | Coffee germplasm, Genebanks., |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/885759 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Embrapa network for Brazilian plant genetic resources conservation.
by: ALVES, A. A. C., et al.
Published: (2019-01-22) -
ISPC Assessment of the revised proposal for the Genebank Platform phase 2 (2017-2022)
by: CGIAR Independent Science and Partnership Council
Published: (2016-09-14) -
Effectiveness of cowpea and wild Vigna germplasm distribution and cost implication on their conservation efforts by IITA
by: Manyong, Victor M., et al.
Published: (2012) -
Bancos de germoplasma de café no Brasil: base do melhoramento para produtividade e qualidade.
by: EIRA, M. T. S. da, et al.
Published: (2011-11-16T11:11:11Z) -
ISPC Note on the Proposal for Financial Support to the CGIAR Centre Genebanks
by: CGIAR Independent Science and Partnership Council
Published: (2011-04-01)