Production of Bacillus thuringiensis biopesticide using commercial lab medium and agricultural by-products as nutrient sources.
Bacillus thuringiensis (Bt) is a Gram-positive bacterium naturally found in soil, water and grain dust, and can be cultivated in liquid, solid and semi-solid media. The objective of this work was to test different media to grow B. thuringiensis. The seed culture (strain 344, B. thuringiensis tolworthi, belonging to Embrapa Maize and Sorghum Microorganism Bank) was produced using shake flasks and grown in LB medium plus salts during 18 hours, incubated on a rotary shaker at 200 revolutions per minute (rpm) at 30oC for 96 hours. Medium 1 was composed of: Luria Bertani (LB) plus salts (FeSO4, ZnSO4, MnSO4, MgSO4), and 0.2% glucose; medium 2 was composed of 1.5% glucose, 0.5% soybean flour plus salts; and medium 3 was composed of liquid swine manure at 4% and 0.2% glucose. All three media were sterilized and inoculated with B. thuringiensis tolwothi (seed culture) atastirrer speed of 200rpm, for 96 hours at 30oC. The pH was measured at regular intervals, viable spores were counted as c.f.u/mL, cell mass expressed in g/L- lyophilized, and spore counting per mL of medium. All three media showed pH variation during the fermentation process. Media 1 and 2 showed a tendency to shift toward a basic pH and medium 3 to an acidic pH. Media 1 and 2 showed the highest number of viable spores, 2.0 x 108 c.f.u/mL, within the 96 hours of incubation, however medium 2 showed a biomass dry weight of 1.18g/L. During the fermentation period, medium 1 showed the highest spore concentration, 1.4 x 109 spores/mL after 96h of fermentation. Efficiency against S. frugiperda first instar larvae showed that all Bt produced in all three media killed above 60% in the highest concentrations.
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Format: | Artigo de periódico biblioteca |
Language: | English eng |
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2011-01-28
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Subjects: | Biocontrole, Inseticida microbiano., Praga de planta, Inseticida, Spodoptera Frugiperda., |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/874936 http://dx.doi.org/10.18512/1980-6477/rbms.v9n1p1-11 |
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dig-alice-doc-8749362017-08-16T03:16:32Z Production of Bacillus thuringiensis biopesticide using commercial lab medium and agricultural by-products as nutrient sources. VALICENTE, F. H. TUELHER, E. de S. LEITE, M. I. S. FREIRE, F. L. VIEIRA, C. M. FERNANDO HERCOS VALICENTE, CNPMS. Biocontrole Inseticida microbiano. Praga de planta Inseticida Spodoptera Frugiperda. Bacillus thuringiensis (Bt) is a Gram-positive bacterium naturally found in soil, water and grain dust, and can be cultivated in liquid, solid and semi-solid media. The objective of this work was to test different media to grow B. thuringiensis. The seed culture (strain 344, B. thuringiensis tolworthi, belonging to Embrapa Maize and Sorghum Microorganism Bank) was produced using shake flasks and grown in LB medium plus salts during 18 hours, incubated on a rotary shaker at 200 revolutions per minute (rpm) at 30oC for 96 hours. Medium 1 was composed of: Luria Bertani (LB) plus salts (FeSO4, ZnSO4, MnSO4, MgSO4), and 0.2% glucose; medium 2 was composed of 1.5% glucose, 0.5% soybean flour plus salts; and medium 3 was composed of liquid swine manure at 4% and 0.2% glucose. All three media were sterilized and inoculated with B. thuringiensis tolwothi (seed culture) atastirrer speed of 200rpm, for 96 hours at 30oC. The pH was measured at regular intervals, viable spores were counted as c.f.u/mL, cell mass expressed in g/L- lyophilized, and spore counting per mL of medium. All three media showed pH variation during the fermentation process. Media 1 and 2 showed a tendency to shift toward a basic pH and medium 3 to an acidic pH. Media 1 and 2 showed the highest number of viable spores, 2.0 x 108 c.f.u/mL, within the 96 hours of incubation, however medium 2 showed a biomass dry weight of 1.18g/L. During the fermentation period, medium 1 showed the highest spore concentration, 1.4 x 109 spores/mL after 96h of fermentation. Efficiency against S. frugiperda first instar larvae showed that all Bt produced in all three media killed above 60% in the highest concentrations. 2011-04-10T11:11:11Z 2011-04-10T11:11:11Z 2011-01-28 2010 2017-05-19T11:11:11Z Artigo de periódico Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 9, n. 1, p. 1-11, 2010. http://www.alice.cnptia.embrapa.br/alice/handle/doc/874936 http://dx.doi.org/10.18512/1980-6477/rbms.v9n1p1-11 en eng openAccess |
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Biocontrole Inseticida microbiano. Praga de planta Inseticida Spodoptera Frugiperda. Biocontrole Inseticida microbiano. Praga de planta Inseticida Spodoptera Frugiperda. |
spellingShingle |
Biocontrole Inseticida microbiano. Praga de planta Inseticida Spodoptera Frugiperda. Biocontrole Inseticida microbiano. Praga de planta Inseticida Spodoptera Frugiperda. VALICENTE, F. H. TUELHER, E. de S. LEITE, M. I. S. FREIRE, F. L. VIEIRA, C. M. Production of Bacillus thuringiensis biopesticide using commercial lab medium and agricultural by-products as nutrient sources. |
description |
Bacillus thuringiensis (Bt) is a Gram-positive bacterium naturally found in soil, water and grain dust, and can be cultivated in liquid, solid and semi-solid media. The objective of this work was to test different media to grow B. thuringiensis. The seed culture (strain 344, B. thuringiensis tolworthi, belonging to Embrapa Maize and Sorghum Microorganism Bank) was produced using shake flasks and grown in LB medium plus salts during 18 hours, incubated on a rotary shaker at 200 revolutions per minute (rpm) at 30oC for 96 hours. Medium 1 was composed of: Luria Bertani (LB) plus salts (FeSO4, ZnSO4, MnSO4, MgSO4), and 0.2% glucose; medium 2 was composed of 1.5% glucose, 0.5% soybean flour plus salts; and medium 3 was composed of liquid swine manure at 4% and 0.2% glucose. All three media were sterilized and inoculated with B. thuringiensis tolwothi (seed culture) atastirrer speed of 200rpm, for 96 hours at 30oC. The pH was measured at regular intervals, viable spores were counted as c.f.u/mL, cell mass expressed in g/L- lyophilized, and spore counting per mL of medium. All three media showed pH variation during the fermentation process. Media 1 and 2 showed a tendency to shift toward a basic pH and medium 3 to an acidic pH. Media 1 and 2 showed the highest number of viable spores, 2.0 x 108 c.f.u/mL, within the 96 hours of incubation, however medium 2 showed a biomass dry weight of 1.18g/L. During the fermentation period, medium 1 showed the highest spore concentration, 1.4 x 109 spores/mL after 96h of fermentation. Efficiency against S. frugiperda first instar larvae showed that all Bt produced in all three media killed above 60% in the highest concentrations. |
author2 |
FERNANDO HERCOS VALICENTE, CNPMS. |
author_facet |
FERNANDO HERCOS VALICENTE, CNPMS. VALICENTE, F. H. TUELHER, E. de S. LEITE, M. I. S. FREIRE, F. L. VIEIRA, C. M. |
format |
Artigo de periódico |
topic_facet |
Biocontrole Inseticida microbiano. Praga de planta Inseticida Spodoptera Frugiperda. |
author |
VALICENTE, F. H. TUELHER, E. de S. LEITE, M. I. S. FREIRE, F. L. VIEIRA, C. M. |
author_sort |
VALICENTE, F. H. |
title |
Production of Bacillus thuringiensis biopesticide using commercial lab medium and agricultural by-products as nutrient sources. |
title_short |
Production of Bacillus thuringiensis biopesticide using commercial lab medium and agricultural by-products as nutrient sources. |
title_full |
Production of Bacillus thuringiensis biopesticide using commercial lab medium and agricultural by-products as nutrient sources. |
title_fullStr |
Production of Bacillus thuringiensis biopesticide using commercial lab medium and agricultural by-products as nutrient sources. |
title_full_unstemmed |
Production of Bacillus thuringiensis biopesticide using commercial lab medium and agricultural by-products as nutrient sources. |
title_sort |
production of bacillus thuringiensis biopesticide using commercial lab medium and agricultural by-products as nutrient sources. |
publishDate |
2011-01-28 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/874936 http://dx.doi.org/10.18512/1980-6477/rbms.v9n1p1-11 |
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