Production of Bacillus thuringiensis biopesticide using commercial lab medium and agricultural by-products as nutrient sources.

Bacillus thuringiensis (Bt) is a Gram-positive bacterium naturally found in soil, water and grain dust, and can be cultivated in liquid, solid and semi-solid media. The objective of this work was to test different media to grow B. thuringiensis. The seed culture (strain 344, B. thuringiensis tolworthi, belonging to Embrapa Maize and Sorghum Microorganism Bank) was produced using shake flasks and grown in LB medium plus salts during 18 hours, incubated on a rotary shaker at 200 revolutions per minute (rpm) at 30oC for 96 hours. Medium 1 was composed of: Luria Bertani (LB) plus salts (FeSO4, ZnSO4, MnSO4, MgSO4), and 0.2% glucose; medium 2 was composed of 1.5% glucose, 0.5% soybean flour plus salts; and medium 3 was composed of liquid swine manure at 4% and 0.2% glucose. All three media were sterilized and inoculated with B. thuringiensis tolwothi (seed culture) atastirrer speed of 200rpm, for 96 hours at 30oC. The pH was measured at regular intervals, viable spores were counted as c.f.u/mL, cell mass expressed in g/L- lyophilized, and spore counting per mL of medium. All three media showed pH variation during the fermentation process. Media 1 and 2 showed a tendency to shift toward a basic pH and medium 3 to an acidic pH. Media 1 and 2 showed the highest number of viable spores, 2.0 x 108 c.f.u/mL, within the 96 hours of incubation, however medium 2 showed a biomass dry weight of 1.18g/L. During the fermentation period, medium 1 showed the highest spore concentration, 1.4 x 109 spores/mL after 96h of fermentation. Efficiency against S. frugiperda first instar larvae showed that all Bt produced in all three media killed above 60% in the highest concentrations.

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Bibliographic Details
Main Authors: VALICENTE, F. H., TUELHER, E. de S., LEITE, M. I. S., FREIRE, F. L., VIEIRA, C. M.
Other Authors: FERNANDO HERCOS VALICENTE, CNPMS.
Format: Artigo de periódico biblioteca
Language:English
eng
Published: 2011-01-28
Subjects:Biocontrole, Inseticida microbiano., Praga de planta, Inseticida, Spodoptera Frugiperda.,
Online Access:http://www.alice.cnptia.embrapa.br/alice/handle/doc/874936
http://dx.doi.org/10.18512/1980-6477/rbms.v9n1p1-11
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spelling dig-alice-doc-8749362017-08-16T03:16:32Z Production of Bacillus thuringiensis biopesticide using commercial lab medium and agricultural by-products as nutrient sources. VALICENTE, F. H. TUELHER, E. de S. LEITE, M. I. S. FREIRE, F. L. VIEIRA, C. M. FERNANDO HERCOS VALICENTE, CNPMS. Biocontrole Inseticida microbiano. Praga de planta Inseticida Spodoptera Frugiperda. Bacillus thuringiensis (Bt) is a Gram-positive bacterium naturally found in soil, water and grain dust, and can be cultivated in liquid, solid and semi-solid media. The objective of this work was to test different media to grow B. thuringiensis. The seed culture (strain 344, B. thuringiensis tolworthi, belonging to Embrapa Maize and Sorghum Microorganism Bank) was produced using shake flasks and grown in LB medium plus salts during 18 hours, incubated on a rotary shaker at 200 revolutions per minute (rpm) at 30oC for 96 hours. Medium 1 was composed of: Luria Bertani (LB) plus salts (FeSO4, ZnSO4, MnSO4, MgSO4), and 0.2% glucose; medium 2 was composed of 1.5% glucose, 0.5% soybean flour plus salts; and medium 3 was composed of liquid swine manure at 4% and 0.2% glucose. All three media were sterilized and inoculated with B. thuringiensis tolwothi (seed culture) atastirrer speed of 200rpm, for 96 hours at 30oC. The pH was measured at regular intervals, viable spores were counted as c.f.u/mL, cell mass expressed in g/L- lyophilized, and spore counting per mL of medium. All three media showed pH variation during the fermentation process. Media 1 and 2 showed a tendency to shift toward a basic pH and medium 3 to an acidic pH. Media 1 and 2 showed the highest number of viable spores, 2.0 x 108 c.f.u/mL, within the 96 hours of incubation, however medium 2 showed a biomass dry weight of 1.18g/L. During the fermentation period, medium 1 showed the highest spore concentration, 1.4 x 109 spores/mL after 96h of fermentation. Efficiency against S. frugiperda first instar larvae showed that all Bt produced in all three media killed above 60% in the highest concentrations. 2011-04-10T11:11:11Z 2011-04-10T11:11:11Z 2011-01-28 2010 2017-05-19T11:11:11Z Artigo de periódico Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 9, n. 1, p. 1-11, 2010. http://www.alice.cnptia.embrapa.br/alice/handle/doc/874936 http://dx.doi.org/10.18512/1980-6477/rbms.v9n1p1-11 en eng openAccess
institution EMBRAPA
collection DSpace
country Brasil
countrycode BR
component Bibliográfico
access En linea
databasecode dig-alice
tag biblioteca
region America del Sur
libraryname Sistema de bibliotecas de EMBRAPA
language English
eng
topic Biocontrole
Inseticida microbiano.
Praga de planta
Inseticida
Spodoptera Frugiperda.
Biocontrole
Inseticida microbiano.
Praga de planta
Inseticida
Spodoptera Frugiperda.
spellingShingle Biocontrole
Inseticida microbiano.
Praga de planta
Inseticida
Spodoptera Frugiperda.
Biocontrole
Inseticida microbiano.
Praga de planta
Inseticida
Spodoptera Frugiperda.
VALICENTE, F. H.
TUELHER, E. de S.
LEITE, M. I. S.
FREIRE, F. L.
VIEIRA, C. M.
Production of Bacillus thuringiensis biopesticide using commercial lab medium and agricultural by-products as nutrient sources.
description Bacillus thuringiensis (Bt) is a Gram-positive bacterium naturally found in soil, water and grain dust, and can be cultivated in liquid, solid and semi-solid media. The objective of this work was to test different media to grow B. thuringiensis. The seed culture (strain 344, B. thuringiensis tolworthi, belonging to Embrapa Maize and Sorghum Microorganism Bank) was produced using shake flasks and grown in LB medium plus salts during 18 hours, incubated on a rotary shaker at 200 revolutions per minute (rpm) at 30oC for 96 hours. Medium 1 was composed of: Luria Bertani (LB) plus salts (FeSO4, ZnSO4, MnSO4, MgSO4), and 0.2% glucose; medium 2 was composed of 1.5% glucose, 0.5% soybean flour plus salts; and medium 3 was composed of liquid swine manure at 4% and 0.2% glucose. All three media were sterilized and inoculated with B. thuringiensis tolwothi (seed culture) atastirrer speed of 200rpm, for 96 hours at 30oC. The pH was measured at regular intervals, viable spores were counted as c.f.u/mL, cell mass expressed in g/L- lyophilized, and spore counting per mL of medium. All three media showed pH variation during the fermentation process. Media 1 and 2 showed a tendency to shift toward a basic pH and medium 3 to an acidic pH. Media 1 and 2 showed the highest number of viable spores, 2.0 x 108 c.f.u/mL, within the 96 hours of incubation, however medium 2 showed a biomass dry weight of 1.18g/L. During the fermentation period, medium 1 showed the highest spore concentration, 1.4 x 109 spores/mL after 96h of fermentation. Efficiency against S. frugiperda first instar larvae showed that all Bt produced in all three media killed above 60% in the highest concentrations.
author2 FERNANDO HERCOS VALICENTE, CNPMS.
author_facet FERNANDO HERCOS VALICENTE, CNPMS.
VALICENTE, F. H.
TUELHER, E. de S.
LEITE, M. I. S.
FREIRE, F. L.
VIEIRA, C. M.
format Artigo de periódico
topic_facet Biocontrole
Inseticida microbiano.
Praga de planta
Inseticida
Spodoptera Frugiperda.
author VALICENTE, F. H.
TUELHER, E. de S.
LEITE, M. I. S.
FREIRE, F. L.
VIEIRA, C. M.
author_sort VALICENTE, F. H.
title Production of Bacillus thuringiensis biopesticide using commercial lab medium and agricultural by-products as nutrient sources.
title_short Production of Bacillus thuringiensis biopesticide using commercial lab medium and agricultural by-products as nutrient sources.
title_full Production of Bacillus thuringiensis biopesticide using commercial lab medium and agricultural by-products as nutrient sources.
title_fullStr Production of Bacillus thuringiensis biopesticide using commercial lab medium and agricultural by-products as nutrient sources.
title_full_unstemmed Production of Bacillus thuringiensis biopesticide using commercial lab medium and agricultural by-products as nutrient sources.
title_sort production of bacillus thuringiensis biopesticide using commercial lab medium and agricultural by-products as nutrient sources.
publishDate 2011-01-28
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/874936
http://dx.doi.org/10.18512/1980-6477/rbms.v9n1p1-11
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