mRNA profile of Nellore calves after primary infection with Haemonchus placei.
Haemonchus parasites are responsible for many losses in animal production. However, few studies are available, especially of zebu cattle. In this study, we investigated mRNA differences of immune response genes in naïve Nellore calves infected with Haemonchus placei, relating these differences to patterns of cellular infiltrate. Calves were infected with 15,000 H. placei L3 larvae and after 7 days lymph node and abomasum tissues were collected. IL-2, IL-4, IL-8, IL-12, IL-13, IFN-?, MCP-1, lysozyme, pepsinogen and TNF-? genes were evaluated by qPCR. Mast cells, eosinophils and globular leukocytes were counted by abomasum histology. In the infected group, IL-4, IL-13 and TNF-? were up-regulated in the abomasal lymph node. In the abomasum, IL-13 increased and TNF-? was down-regulated (p<0.05). No differences were detected for mast cells and eosinophil counts in abomasal tissue (p>0.05). We conclude that for this infection time, there was Th2 polarization but that cellular infiltrate in abomasal tissue takes longer to develop.
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Format: | Artigo de periódico biblioteca |
Language: | Ingles English |
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2010-12-15
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Subjects: | MRNA profile, Gastrointestinal parasites, Bos Indicus, Haemonchus placei, Nellore, beef cattle, cytokines, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/870087 |
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dig-alice-doc-8700872023-03-13T11:51:57Z mRNA profile of Nellore calves after primary infection with Haemonchus placei. IBELLI, A. M. G. NAKATA, L. C. ANDRÉO, R. COUTINHO, L. L. OLIVEIRA, M. C. de S. AMARANTE, A. F. T. FURLONG, J. ZAROS, L. G. REGITANO, L. C. de A. ADRIANA M. G. IBELLI, UFSCar/SÃO CARLOS, SP; L. C. NAKATA, UFSCar; R. ANDRÉO, UNESP; L. L. COUTINHO, USP-ESALQ; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; A. F. T. AMARANTE, UNESP; JOHN FURLONG, CNPGL; L. G. ZAROS, UFRGN; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE. MRNA profile Gastrointestinal parasites Bos Indicus Haemonchus placei Nellore beef cattle cytokines Haemonchus parasites are responsible for many losses in animal production. However, few studies are available, especially of zebu cattle. In this study, we investigated mRNA differences of immune response genes in naïve Nellore calves infected with Haemonchus placei, relating these differences to patterns of cellular infiltrate. Calves were infected with 15,000 H. placei L3 larvae and after 7 days lymph node and abomasum tissues were collected. IL-2, IL-4, IL-8, IL-12, IL-13, IFN-?, MCP-1, lysozyme, pepsinogen and TNF-? genes were evaluated by qPCR. Mast cells, eosinophils and globular leukocytes were counted by abomasum histology. In the infected group, IL-4, IL-13 and TNF-? were up-regulated in the abomasal lymph node. In the abomasum, IL-13 increased and TNF-? was down-regulated (p<0.05). No differences were detected for mast cells and eosinophil counts in abomasal tissue (p>0.05). We conclude that for this infection time, there was Th2 polarization but that cellular infiltrate in abomasal tissue takes longer to develop. 2023-03-13T11:51:57Z 2023-03-13T11:51:57Z 2010-12-15 2011 Artigo de periódico Veterinary Parasitology, v. 176, n. 2-3, p. 195-200, mar. 2011. http://www.alice.cnptia.embrapa.br/alice/handle/doc/870087 10.1016/j.vetpar.2010.11.013 Ingles en openAccess |
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MRNA profile Gastrointestinal parasites Bos Indicus Haemonchus placei Nellore beef cattle cytokines MRNA profile Gastrointestinal parasites Bos Indicus Haemonchus placei Nellore beef cattle cytokines |
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MRNA profile Gastrointestinal parasites Bos Indicus Haemonchus placei Nellore beef cattle cytokines MRNA profile Gastrointestinal parasites Bos Indicus Haemonchus placei Nellore beef cattle cytokines IBELLI, A. M. G. NAKATA, L. C. ANDRÉO, R. COUTINHO, L. L. OLIVEIRA, M. C. de S. AMARANTE, A. F. T. FURLONG, J. ZAROS, L. G. REGITANO, L. C. de A. mRNA profile of Nellore calves after primary infection with Haemonchus placei. |
description |
Haemonchus parasites are responsible for many losses in animal production. However, few studies are available, especially of zebu cattle. In this study, we investigated mRNA differences of immune response genes in naïve Nellore calves infected with Haemonchus placei, relating these differences to patterns of cellular infiltrate. Calves were infected with 15,000 H. placei L3 larvae and after 7 days lymph node and abomasum tissues were collected. IL-2, IL-4, IL-8, IL-12, IL-13, IFN-?, MCP-1, lysozyme, pepsinogen and TNF-? genes were evaluated by qPCR. Mast cells, eosinophils and globular leukocytes were counted by abomasum histology. In the infected group, IL-4, IL-13 and TNF-? were up-regulated in the abomasal lymph node. In the abomasum, IL-13 increased and TNF-? was down-regulated (p<0.05). No differences were detected for mast cells and eosinophil counts in abomasal tissue (p>0.05). We conclude that for this infection time, there was Th2 polarization but that cellular infiltrate in abomasal tissue takes longer to develop. |
author2 |
ADRIANA M. G. IBELLI, UFSCar/SÃO CARLOS, SP; L. C. NAKATA, UFSCar; R. ANDRÉO, UNESP; L. L. COUTINHO, USP-ESALQ; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; A. F. T. AMARANTE, UNESP; JOHN FURLONG, CNPGL; L. G. ZAROS, UFRGN; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE. |
author_facet |
ADRIANA M. G. IBELLI, UFSCar/SÃO CARLOS, SP; L. C. NAKATA, UFSCar; R. ANDRÉO, UNESP; L. L. COUTINHO, USP-ESALQ; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; A. F. T. AMARANTE, UNESP; JOHN FURLONG, CNPGL; L. G. ZAROS, UFRGN; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE. IBELLI, A. M. G. NAKATA, L. C. ANDRÉO, R. COUTINHO, L. L. OLIVEIRA, M. C. de S. AMARANTE, A. F. T. FURLONG, J. ZAROS, L. G. REGITANO, L. C. de A. |
format |
Artigo de periódico |
topic_facet |
MRNA profile Gastrointestinal parasites Bos Indicus Haemonchus placei Nellore beef cattle cytokines |
author |
IBELLI, A. M. G. NAKATA, L. C. ANDRÉO, R. COUTINHO, L. L. OLIVEIRA, M. C. de S. AMARANTE, A. F. T. FURLONG, J. ZAROS, L. G. REGITANO, L. C. de A. |
author_sort |
IBELLI, A. M. G. |
title |
mRNA profile of Nellore calves after primary infection with Haemonchus placei. |
title_short |
mRNA profile of Nellore calves after primary infection with Haemonchus placei. |
title_full |
mRNA profile of Nellore calves after primary infection with Haemonchus placei. |
title_fullStr |
mRNA profile of Nellore calves after primary infection with Haemonchus placei. |
title_full_unstemmed |
mRNA profile of Nellore calves after primary infection with Haemonchus placei. |
title_sort |
mrna profile of nellore calves after primary infection with haemonchus placei. |
publishDate |
2010-12-15 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/870087 |
work_keys_str_mv |
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