Effect of copper on the activation of the acid phosphatase from the green algae Pseudokirchneriella subcapitata.
The in vitro activation effect of copper on the acid phosphatase of the green algae Pseudokirch- neriella subcapitata (formely Selenastrum capricor- nutum) under preincubation condition. Apparent Michaelis constant values of 1.21 and 0.37 mM, and activation energy values of 26.8 and 13.6 kJ mol -1 were determined in the absence and in the presence of 0.2 mM Cu2?, respectively. The dissociation constant value for Cu2? binding to the enzyme was determined to be 22.04 lM. The decrease of the apparent Michaelis constant (Km) and activation energy values in the presence of Cu2? correlates well with its activating effect on the acid phosphatase activity. This propriety could be used as a sensitive bioindicator for copper in environmental samples.
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2009-12-02
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Subjects: | Ecotoxicologia, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/577024 |
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dig-alice-doc-5770242017-08-16T03:29:20Z Effect of copper on the activation of the acid phosphatase from the green algae Pseudokirchneriella subcapitata. JONSSON, C. M. AOYAMA, H. CLAUDIO MARTIN JONSSON, CNPMA; HIROSHI AOYAMA, IB-UNICAMP. Ecotoxicologia The in vitro activation effect of copper on the acid phosphatase of the green algae Pseudokirch- neriella subcapitata (formely Selenastrum capricor- nutum) under preincubation condition. Apparent Michaelis constant values of 1.21 and 0.37 mM, and activation energy values of 26.8 and 13.6 kJ mol -1 were determined in the absence and in the presence of 0.2 mM Cu2?, respectively. The dissociation constant value for Cu2? binding to the enzyme was determined to be 22.04 lM. The decrease of the apparent Michaelis constant (Km) and activation energy values in the presence of Cu2? correlates well with its activating effect on the acid phosphatase activity. This propriety could be used as a sensitive bioindicator for copper in environmental samples. 2016-06-04T09:06:45Z 2016-06-04T09:06:45Z 2009-12-02 2010 2016-06-04T09:06:45Z Separatas Biometals, v. 23, n. 1, p. 93-98, Sep. 2010. http://www.alice.cnptia.embrapa.br/alice/handle/doc/577024 10.1007/s10534-009-9270-z pt_BR por openAccess |
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Ecotoxicologia Ecotoxicologia JONSSON, C. M. AOYAMA, H. Effect of copper on the activation of the acid phosphatase from the green algae Pseudokirchneriella subcapitata. |
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The in vitro activation effect of copper on the acid phosphatase of the green algae Pseudokirch- neriella subcapitata (formely Selenastrum capricor- nutum) under preincubation condition. Apparent Michaelis constant values of 1.21 and 0.37 mM, and activation energy values of 26.8 and 13.6 kJ mol -1 were determined in the absence and in the presence of 0.2 mM Cu2?, respectively. The dissociation constant value for Cu2? binding to the enzyme was determined to be 22.04 lM. The decrease of the apparent Michaelis constant (Km) and activation energy values in the presence of Cu2? correlates well with its activating effect on the acid phosphatase activity. This propriety could be used as a sensitive bioindicator for copper in environmental samples. |
author2 |
CLAUDIO MARTIN JONSSON, CNPMA; HIROSHI AOYAMA, IB-UNICAMP. |
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CLAUDIO MARTIN JONSSON, CNPMA; HIROSHI AOYAMA, IB-UNICAMP. JONSSON, C. M. AOYAMA, H. |
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Ecotoxicologia |
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JONSSON, C. M. AOYAMA, H. |
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JONSSON, C. M. |
title |
Effect of copper on the activation of the acid phosphatase from the green algae Pseudokirchneriella subcapitata. |
title_short |
Effect of copper on the activation of the acid phosphatase from the green algae Pseudokirchneriella subcapitata. |
title_full |
Effect of copper on the activation of the acid phosphatase from the green algae Pseudokirchneriella subcapitata. |
title_fullStr |
Effect of copper on the activation of the acid phosphatase from the green algae Pseudokirchneriella subcapitata. |
title_full_unstemmed |
Effect of copper on the activation of the acid phosphatase from the green algae Pseudokirchneriella subcapitata. |
title_sort |
effect of copper on the activation of the acid phosphatase from the green algae pseudokirchneriella subcapitata. |
publishDate |
2009-12-02 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/577024 |
work_keys_str_mv |
AT jonssoncm effectofcopperontheactivationoftheacidphosphatasefromthegreenalgaepseudokirchneriellasubcapitata AT aoyamah effectofcopperontheactivationoftheacidphosphatasefromthegreenalgaepseudokirchneriellasubcapitata |
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