Influence of Pseudomonas putida AF7 inoculation on soil enzymes.

There has been some concern about the environmental impact of microbial agents. Pseudomonas may be used as bioremediator and as biopesticide. In this study, we report the use of soil enzyme assays as biological indicator of possible negative effects in soil functioning after the P. putida AF7 inoculation. For that, P. putida AF7 was originally isolated from the rizosphere of rice and was inoculated on three soil types: Rhodic Hapludox (RH), Typic Hapludox (TH); and Arenic Hapludult (AH). The acid phosphatase, b-glucosidase and protease enzymes activities were measured for three period of evaluation (7, 14 and 21 days). In general, the enzymatic activities pre- sented variation among the tested soils. The highest activities of b-glucosidase and acid phosphatase were observed in the RH and AH soils, while the protease activity was higher in the TH soil. Also, the soil charac- teristics were measured for each plot. The activity of enzymes from the carbon cycle was positively correlated with the N and the P and the enzyme from the nitrogen cycle was negatively correlated with N and C.org. The presented data indicate that soil biochemical properties can be an useful tool for use as an indicator of soil perturba- tions by microbial inoculation in a risk assessment.

Saved in:
Bibliographic Details
Main Authors: SILVA, C. M. M. S., CASTRO, V. L. S. S. de, OLIVEIRA, P. R., MAIA, A. de H. N.
Other Authors: CELIA MARIA MAGANHOTTO S SILVA, CNPMA; VERA LUCIA SCHERHOLZ S DE CASTRO, CNPMA; PABLO ROBERTO DE OLIVEIRA, Estagiário - Embrapa Meio Ambiente; ALINE DE HOLANDA NUNES MAIA, CNPMA.
Format: Separatas biblioteca
Language:English
eng
Published: 2009-12-01
Subjects:Bioquímica do Solo.,
Online Access:http://www.alice.cnptia.embrapa.br/alice/handle/doc/576823
Tags: Add Tag
No Tags, Be the first to tag this record!
id dig-alice-doc-576823
record_format koha
spelling dig-alice-doc-5768232017-08-16T03:32:52Z Influence of Pseudomonas putida AF7 inoculation on soil enzymes. SILVA, C. M. M. S. CASTRO, V. L. S. S. de OLIVEIRA, P. R. MAIA, A. de H. N. CELIA MARIA MAGANHOTTO S SILVA, CNPMA; VERA LUCIA SCHERHOLZ S DE CASTRO, CNPMA; PABLO ROBERTO DE OLIVEIRA, Estagiário - Embrapa Meio Ambiente; ALINE DE HOLANDA NUNES MAIA, CNPMA. Bioquímica do Solo. There has been some concern about the environmental impact of microbial agents. Pseudomonas may be used as bioremediator and as biopesticide. In this study, we report the use of soil enzyme assays as biological indicator of possible negative effects in soil functioning after the P. putida AF7 inoculation. For that, P. putida AF7 was originally isolated from the rizosphere of rice and was inoculated on three soil types: Rhodic Hapludox (RH), Typic Hapludox (TH); and Arenic Hapludult (AH). The acid phosphatase, b-glucosidase and protease enzymes activities were measured for three period of evaluation (7, 14 and 21 days). In general, the enzymatic activities pre- sented variation among the tested soils. The highest activities of b-glucosidase and acid phosphatase were observed in the RH and AH soils, while the protease activity was higher in the TH soil. Also, the soil charac- teristics were measured for each plot. The activity of enzymes from the carbon cycle was positively correlated with the N and the P and the enzyme from the nitrogen cycle was negatively correlated with N and C.org. The presented data indicate that soil biochemical properties can be an useful tool for use as an indicator of soil perturba- tions by microbial inoculation in a risk assessment. 2016-06-03T12:41:22Z 2016-06-03T12:41:22Z 2009-12-01 2009 2016-06-03T12:41:22Z Separatas Ecotoxicology, v. 18, n .5, p. 1182-1187, 2009. http://www.alice.cnptia.embrapa.br/alice/handle/doc/576823 10.1007/s10646-009-0319-0 en eng openAccess
institution EMBRAPA
collection DSpace
country Brasil
countrycode BR
component Bibliográfico
access En linea
databasecode dig-alice
tag biblioteca
region America del Sur
libraryname Sistema de bibliotecas de EMBRAPA
language English
eng
topic Bioquímica do Solo.
Bioquímica do Solo.
spellingShingle Bioquímica do Solo.
Bioquímica do Solo.
SILVA, C. M. M. S.
CASTRO, V. L. S. S. de
OLIVEIRA, P. R.
MAIA, A. de H. N.
Influence of Pseudomonas putida AF7 inoculation on soil enzymes.
description There has been some concern about the environmental impact of microbial agents. Pseudomonas may be used as bioremediator and as biopesticide. In this study, we report the use of soil enzyme assays as biological indicator of possible negative effects in soil functioning after the P. putida AF7 inoculation. For that, P. putida AF7 was originally isolated from the rizosphere of rice and was inoculated on three soil types: Rhodic Hapludox (RH), Typic Hapludox (TH); and Arenic Hapludult (AH). The acid phosphatase, b-glucosidase and protease enzymes activities were measured for three period of evaluation (7, 14 and 21 days). In general, the enzymatic activities pre- sented variation among the tested soils. The highest activities of b-glucosidase and acid phosphatase were observed in the RH and AH soils, while the protease activity was higher in the TH soil. Also, the soil charac- teristics were measured for each plot. The activity of enzymes from the carbon cycle was positively correlated with the N and the P and the enzyme from the nitrogen cycle was negatively correlated with N and C.org. The presented data indicate that soil biochemical properties can be an useful tool for use as an indicator of soil perturba- tions by microbial inoculation in a risk assessment.
author2 CELIA MARIA MAGANHOTTO S SILVA, CNPMA; VERA LUCIA SCHERHOLZ S DE CASTRO, CNPMA; PABLO ROBERTO DE OLIVEIRA, Estagiário - Embrapa Meio Ambiente; ALINE DE HOLANDA NUNES MAIA, CNPMA.
author_facet CELIA MARIA MAGANHOTTO S SILVA, CNPMA; VERA LUCIA SCHERHOLZ S DE CASTRO, CNPMA; PABLO ROBERTO DE OLIVEIRA, Estagiário - Embrapa Meio Ambiente; ALINE DE HOLANDA NUNES MAIA, CNPMA.
SILVA, C. M. M. S.
CASTRO, V. L. S. S. de
OLIVEIRA, P. R.
MAIA, A. de H. N.
format Separatas
topic_facet Bioquímica do Solo.
author SILVA, C. M. M. S.
CASTRO, V. L. S. S. de
OLIVEIRA, P. R.
MAIA, A. de H. N.
author_sort SILVA, C. M. M. S.
title Influence of Pseudomonas putida AF7 inoculation on soil enzymes.
title_short Influence of Pseudomonas putida AF7 inoculation on soil enzymes.
title_full Influence of Pseudomonas putida AF7 inoculation on soil enzymes.
title_fullStr Influence of Pseudomonas putida AF7 inoculation on soil enzymes.
title_full_unstemmed Influence of Pseudomonas putida AF7 inoculation on soil enzymes.
title_sort influence of pseudomonas putida af7 inoculation on soil enzymes.
publishDate 2009-12-01
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/576823
work_keys_str_mv AT silvacmms influenceofpseudomonasputidaaf7inoculationonsoilenzymes
AT castrovlssde influenceofpseudomonasputidaaf7inoculationonsoilenzymes
AT oliveirapr influenceofpseudomonasputidaaf7inoculationonsoilenzymes
AT maiaadehn influenceofpseudomonasputidaaf7inoculationonsoilenzymes
_version_ 1756022423732355072