Amino acid sequences of proteins from Leptospira serovar pomona.
Abstracts: This report describes a partial amino acid sequences from three putative outer envelope proteins from Leptospira serovar pomona. In order to obtain internal fragments for protein sequencing, enzymatic and chemical digestion was performed. The enzyme clostripain was used to digest the proteins 32 and 45kDa. In situ digestion of 40kDa molecular weight protein was accomplished using cyanogen bromide. The 32kDa protein generated two fragments, one of 21kDa and another of 10kDa that yielded five residues. A fragment of 24 kDa that yielded nineteen residues of amino acids was obtained from 45 kDa protein. A fragment with a molecular weight of 20 kDa, yielding a twenty amino acids sequence from the 40kDa protein.
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Format: | Artigo de periódico biblioteca |
Language: | English eng |
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2000-08-07
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Subjects: | Sequência, Sequence., Doença animal, Aminoácido, Proteína, Leptospira pomona, Leptospirose., Leptospira, Proteins, Amino acids, Animal diseases, Antigens., |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/515320 |
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dig-alice-doc-5153202017-08-16T01:47:46Z Amino acid sequences of proteins from Leptospira serovar pomona. ALVES, F. S. F. LEFEBVRE, R. B. PROBERT, W. FRANCISCO SELMO FERNANDES ALVES, CNPC WILLIAM PROBERT, University of California, Davis/Department of Veterinary Microbiology, Immunology and Pathology, 95616-Davis, CA, USA. RANCE B. LEFEBVRE, University of California, Davis/Department of Veterinary Microbiology, Immunology and Pathology, 95616-Davis, CA, USA Sequência Sequence. Doença animal Aminoácido Proteína Leptospira pomona Leptospirose. Leptospira Proteins Amino acids Animal diseases Antigens. Abstracts: This report describes a partial amino acid sequences from three putative outer envelope proteins from Leptospira serovar pomona. In order to obtain internal fragments for protein sequencing, enzymatic and chemical digestion was performed. The enzyme clostripain was used to digest the proteins 32 and 45kDa. In situ digestion of 40kDa molecular weight protein was accomplished using cyanogen bromide. The 32kDa protein generated two fragments, one of 21kDa and another of 10kDa that yielded five residues. A fragment of 24 kDa that yielded nineteen residues of amino acids was obtained from 45 kDa protein. A fragment with a molecular weight of 20 kDa, yielding a twenty amino acids sequence from the 40kDa protein. 2011-04-10T11:11:11Z 2011-04-10T11:11:11Z 2000-08-07 2000 2019-09-25T11:11:11Z Artigo de periódico Memórias do Instituto Oswaldo Cruz, p. 95, n. 4, p. 503-504, jul./ago. 2000. http://www.alice.cnptia.embrapa.br/alice/handle/doc/515320 en eng openAccess |
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Sequência Sequence. Doença animal Aminoácido Proteína Leptospira pomona Leptospirose. Leptospira Proteins Amino acids Animal diseases Antigens. Sequência Sequence. Doença animal Aminoácido Proteína Leptospira pomona Leptospirose. Leptospira Proteins Amino acids Animal diseases Antigens. |
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Sequência Sequence. Doença animal Aminoácido Proteína Leptospira pomona Leptospirose. Leptospira Proteins Amino acids Animal diseases Antigens. Sequência Sequence. Doença animal Aminoácido Proteína Leptospira pomona Leptospirose. Leptospira Proteins Amino acids Animal diseases Antigens. ALVES, F. S. F. LEFEBVRE, R. B. PROBERT, W. Amino acid sequences of proteins from Leptospira serovar pomona. |
description |
Abstracts: This report describes a partial amino acid sequences from three putative outer envelope proteins from Leptospira serovar pomona. In order to obtain internal fragments for protein sequencing, enzymatic and chemical digestion was performed. The enzyme clostripain was used to digest the proteins 32 and 45kDa. In situ digestion of 40kDa molecular weight protein was accomplished using cyanogen bromide. The 32kDa protein generated two fragments, one of 21kDa and another of 10kDa that yielded five residues. A fragment of 24 kDa that yielded nineteen residues of amino acids was obtained from 45 kDa protein. A fragment with a molecular weight of 20 kDa, yielding a twenty amino acids sequence from the 40kDa protein. |
author2 |
FRANCISCO SELMO FERNANDES ALVES, CNPC |
author_facet |
FRANCISCO SELMO FERNANDES ALVES, CNPC ALVES, F. S. F. LEFEBVRE, R. B. PROBERT, W. |
format |
Artigo de periódico |
topic_facet |
Sequência Sequence. Doença animal Aminoácido Proteína Leptospira pomona Leptospirose. Leptospira Proteins Amino acids Animal diseases Antigens. |
author |
ALVES, F. S. F. LEFEBVRE, R. B. PROBERT, W. |
author_sort |
ALVES, F. S. F. |
title |
Amino acid sequences of proteins from Leptospira serovar pomona. |
title_short |
Amino acid sequences of proteins from Leptospira serovar pomona. |
title_full |
Amino acid sequences of proteins from Leptospira serovar pomona. |
title_fullStr |
Amino acid sequences of proteins from Leptospira serovar pomona. |
title_full_unstemmed |
Amino acid sequences of proteins from Leptospira serovar pomona. |
title_sort |
amino acid sequences of proteins from leptospira serovar pomona. |
publishDate |
2000-08-07 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/515320 |
work_keys_str_mv |
AT alvesfsf aminoacidsequencesofproteinsfromleptospiraserovarpomona AT lefebvrerb aminoacidsequencesofproteinsfromleptospiraserovarpomona AT probertw aminoacidsequencesofproteinsfromleptospiraserovarpomona |
_version_ |
1756015414889938944 |