Localization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques.
The etiological agent of maize white spot (MWS) disease has been a subject of controversy and discussion. Initially the disease was described as Phaeosphaeria leaf spot caused by Phaeosphaeria maydis. Other authors have suggested the existence of different fungal species causing similar symptoms. Recently, a bacterium, Pantoea ananatis, was described as the causal agent of this disease. The purpose of this study was to offer additional information on the correct etiology of this disease by providing visual evidence of the presence of the bacterium in the interior of the MWS lesions by using transmission electron microscopy (TEM) and molecular techniques. The TEM allowed visualization of a large amount of bacteria in the intercellular spaces of lesions collected from both artificially and naturally infected plants. Fungal structures were not visualized in young lesions. Bacterial primers for the 16S rRNA and rpoB genes were used in PCR reactions 10 amplify DNA extracted from water-soaked (young) and necrotic lesions. The universal fungal oligonucleotide ITS4 was also included to identify the possible presence of funga! structures inside lesions. Positive PCR products from water-soaked lesions, both from naturally and artificially inoculated plants, were produced with bacterial primers, whereas no amplification was observed when ITS4 oligonucleotide was used. On the other hand, DNA amplification with ITS4 primer was observed when DNA was isolated from necrotic (old) lesions. These results reinforced previous report of P. ananatis as the primary pathogen and the hypothesis that fungal species may colonize lesions pre-established by P. ananatis.
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Format: | Artigo de periódico biblioteca |
Language: | English eng |
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2008-06-17
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Subjects: | Doença bacteriana do milho, doenças foliares do milho, Maize bacterial disease, Maize leaf disease., Milho, Zea Mays., |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/491172 |
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dig-alice-doc-4911722017-08-15T23:20:51Z Localization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques. BOMFETI, C. A. SOUZA-PACCOLA, E. A. MASSOLA JUNIOR, N. S. MARRIEL, I. E. MEIRELLES, W. F. CASELA, C. R. PACCOLA-MEIRELLES, L. D. Cleide A. Bomfeti, UEL; Edneia A. Sopuza-Paccola, UEL; Nelson S. Massola Junior, Esalq; IVANILDO EVODIO MARRIEL, CNPMS; WALTER FERNANDES MEIRELLES, CNPMS; Carlos Roberto Casela, CNPMS; Luzia D. Paccola-Meirelles, UEL. Doença bacteriana do milho doenças foliares do milho Maize bacterial disease Maize leaf disease. Milho Zea Mays. The etiological agent of maize white spot (MWS) disease has been a subject of controversy and discussion. Initially the disease was described as Phaeosphaeria leaf spot caused by Phaeosphaeria maydis. Other authors have suggested the existence of different fungal species causing similar symptoms. Recently, a bacterium, Pantoea ananatis, was described as the causal agent of this disease. The purpose of this study was to offer additional information on the correct etiology of this disease by providing visual evidence of the presence of the bacterium in the interior of the MWS lesions by using transmission electron microscopy (TEM) and molecular techniques. The TEM allowed visualization of a large amount of bacteria in the intercellular spaces of lesions collected from both artificially and naturally infected plants. Fungal structures were not visualized in young lesions. Bacterial primers for the 16S rRNA and rpoB genes were used in PCR reactions 10 amplify DNA extracted from water-soaked (young) and necrotic lesions. The universal fungal oligonucleotide ITS4 was also included to identify the possible presence of funga! structures inside lesions. Positive PCR products from water-soaked lesions, both from naturally and artificially inoculated plants, were produced with bacterial primers, whereas no amplification was observed when ITS4 oligonucleotide was used. On the other hand, DNA amplification with ITS4 primer was observed when DNA was isolated from necrotic (old) lesions. These results reinforced previous report of P. ananatis as the primary pathogen and the hypothesis that fungal species may colonize lesions pre-established by P. ananatis. 2011-04-10T11:11:11Z 2011-04-10T11:11:11Z 2008-06-17 2008 2018-05-24T11:11:11Z Artigo de periódico Tropical Plant Pathology, Brasília, v. 33, n. 1, p. 63-66, Jan./Feb. 2008. http://www.alice.cnptia.embrapa.br/alice/handle/doc/491172 10.1590/S1982-56762008000100010 en eng openAccess |
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Doença bacteriana do milho doenças foliares do milho Maize bacterial disease Maize leaf disease. Milho Zea Mays. Doença bacteriana do milho doenças foliares do milho Maize bacterial disease Maize leaf disease. Milho Zea Mays. |
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Doença bacteriana do milho doenças foliares do milho Maize bacterial disease Maize leaf disease. Milho Zea Mays. Doença bacteriana do milho doenças foliares do milho Maize bacterial disease Maize leaf disease. Milho Zea Mays. BOMFETI, C. A. SOUZA-PACCOLA, E. A. MASSOLA JUNIOR, N. S. MARRIEL, I. E. MEIRELLES, W. F. CASELA, C. R. PACCOLA-MEIRELLES, L. D. Localization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques. |
description |
The etiological agent of maize white spot (MWS) disease has been a subject of controversy and discussion. Initially the disease was described as Phaeosphaeria leaf spot caused by Phaeosphaeria maydis. Other authors have suggested the existence of different fungal species causing similar symptoms. Recently, a bacterium, Pantoea ananatis, was described as the causal agent of this disease. The purpose of this study was to offer additional information on the correct etiology of this disease by providing visual evidence of the presence of the bacterium in the interior of the MWS lesions by using transmission electron microscopy (TEM) and molecular techniques. The TEM allowed visualization of a large amount of bacteria in the intercellular spaces of lesions collected from both artificially and naturally infected plants. Fungal structures were not visualized in young lesions. Bacterial primers for the 16S rRNA and rpoB genes were used in PCR reactions 10 amplify DNA extracted from water-soaked (young) and necrotic lesions. The universal fungal oligonucleotide ITS4 was also included to identify the possible presence of funga! structures inside lesions. Positive PCR products from water-soaked lesions, both from naturally and artificially inoculated plants, were produced with bacterial primers, whereas no amplification was observed when ITS4 oligonucleotide was used. On the other hand, DNA amplification with ITS4 primer was observed when DNA was isolated from necrotic (old) lesions. These results reinforced previous report of P. ananatis as the primary pathogen and the hypothesis that fungal species may colonize lesions pre-established by P. ananatis. |
author2 |
Cleide A. Bomfeti, UEL; Edneia A. Sopuza-Paccola, UEL; Nelson S. Massola Junior, Esalq; IVANILDO EVODIO MARRIEL, CNPMS; WALTER FERNANDES MEIRELLES, CNPMS; Carlos Roberto Casela, CNPMS; Luzia D. Paccola-Meirelles, UEL. |
author_facet |
Cleide A. Bomfeti, UEL; Edneia A. Sopuza-Paccola, UEL; Nelson S. Massola Junior, Esalq; IVANILDO EVODIO MARRIEL, CNPMS; WALTER FERNANDES MEIRELLES, CNPMS; Carlos Roberto Casela, CNPMS; Luzia D. Paccola-Meirelles, UEL. BOMFETI, C. A. SOUZA-PACCOLA, E. A. MASSOLA JUNIOR, N. S. MARRIEL, I. E. MEIRELLES, W. F. CASELA, C. R. PACCOLA-MEIRELLES, L. D. |
format |
Artigo de periódico |
topic_facet |
Doença bacteriana do milho doenças foliares do milho Maize bacterial disease Maize leaf disease. Milho Zea Mays. |
author |
BOMFETI, C. A. SOUZA-PACCOLA, E. A. MASSOLA JUNIOR, N. S. MARRIEL, I. E. MEIRELLES, W. F. CASELA, C. R. PACCOLA-MEIRELLES, L. D. |
author_sort |
BOMFETI, C. A. |
title |
Localization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques. |
title_short |
Localization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques. |
title_full |
Localization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques. |
title_fullStr |
Localization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques. |
title_full_unstemmed |
Localization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques. |
title_sort |
localization of pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques. |
publishDate |
2008-06-17 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/491172 |
work_keys_str_mv |
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1756014461468016640 |