Genetic maps of Saccharum officinarum L. and Saccharum robustum Brandes & Jew ex grassl.
Genetic analysis was performed in a population composed of 100 F1 individuals derived from a cross between a cultivated sugarcane (S. officinarum La Purple) and its proposed progenitor species (S. robustum "Mol 5829'). Various types (arbitrarily primed-PCR, RFLPs, and AFLPs) of single-dose DNA markers (SDMs) were used to construct genetic linkage maps to both species. The LA Purple map was composed of 341 SDMs, spanning 74 linkage groups and 1.881 cM, white the Mol 5829 map contained 301 SDMs, spanning 65 linkage gropus and 1,189 cM, Transmission genetics in these two species showed incomplete polysomy based on the detection of 15% of SDMs linked in repulsion in LA Purple and 13% of these in Mol 5829. Because of this incomplete polysomy, multiple-dose markers could not be mapped for lack of a genetic model for their segregation. Due to inclusion of RFLP anchor probes, conserved in related species, the resulting maps will serve as useful tools for breeding, ecology, evolution, and molecular biology studies within the Andropogoneae.
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2000-03-14
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Subjects: | Mapeamento, Mapping, Biotehnology., Biotecnologia, Cana de Açúcar, DNA, Genética., genetics, sugarcane., |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/483496 |
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dig-alice-doc-4834962017-08-15T23:49:11Z Genetic maps of Saccharum officinarum L. and Saccharum robustum Brandes & Jew ex grassl. GUIMARAES, C. T. HONEYCUTT, R. J. SILLS, G. R. SOBRAL, W. S. CLAUDIA TEIXEIRA GUIMARAES, CNPMS. Mapeamento Mapping Biotehnology. Biotecnologia Cana de Açúcar DNA Genética. genetics sugarcane. Genetic analysis was performed in a population composed of 100 F1 individuals derived from a cross between a cultivated sugarcane (S. officinarum La Purple) and its proposed progenitor species (S. robustum "Mol 5829'). Various types (arbitrarily primed-PCR, RFLPs, and AFLPs) of single-dose DNA markers (SDMs) were used to construct genetic linkage maps to both species. The LA Purple map was composed of 341 SDMs, spanning 74 linkage groups and 1.881 cM, white the Mol 5829 map contained 301 SDMs, spanning 65 linkage gropus and 1,189 cM, Transmission genetics in these two species showed incomplete polysomy based on the detection of 15% of SDMs linked in repulsion in LA Purple and 13% of these in Mol 5829. Because of this incomplete polysomy, multiple-dose markers could not be mapped for lack of a genetic model for their segregation. Due to inclusion of RFLP anchor probes, conserved in related species, the resulting maps will serve as useful tools for breeding, ecology, evolution, and molecular biology studies within the Andropogoneae. 2012-10-10T01:01:01Z 2012-10-10T01:01:01Z 2000-03-14 1999 2018-06-09T11:11:11Z Parte de livro Genetics and Molecular Biology, Ribeirão Preto, v. 22, n. 1, p. 125-132, 1999. http://www.alice.cnptia.embrapa.br/alice/handle/doc/483496 pt_BR por openAccess |
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Mapeamento Mapping Biotehnology. Biotecnologia Cana de Açúcar DNA Genética. genetics sugarcane. Mapeamento Mapping Biotehnology. Biotecnologia Cana de Açúcar DNA Genética. genetics sugarcane. |
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Mapeamento Mapping Biotehnology. Biotecnologia Cana de Açúcar DNA Genética. genetics sugarcane. Mapeamento Mapping Biotehnology. Biotecnologia Cana de Açúcar DNA Genética. genetics sugarcane. GUIMARAES, C. T. HONEYCUTT, R. J. SILLS, G. R. SOBRAL, W. S. Genetic maps of Saccharum officinarum L. and Saccharum robustum Brandes & Jew ex grassl. |
description |
Genetic analysis was performed in a population composed of 100 F1 individuals derived from a cross between a cultivated sugarcane (S. officinarum La Purple) and its proposed progenitor species (S. robustum "Mol 5829'). Various types (arbitrarily primed-PCR, RFLPs, and AFLPs) of single-dose DNA markers (SDMs) were used to construct genetic linkage maps to both species. The LA Purple map was composed of 341 SDMs, spanning 74 linkage groups and 1.881 cM, white the Mol 5829 map contained 301 SDMs, spanning 65 linkage gropus and 1,189 cM, Transmission genetics in these two species showed incomplete polysomy based on the detection of 15% of SDMs linked in repulsion in LA Purple and 13% of these in Mol 5829. Because of this incomplete polysomy, multiple-dose markers could not be mapped for lack of a genetic model for their segregation. Due to inclusion of RFLP anchor probes, conserved in related species, the resulting maps will serve as useful tools for breeding, ecology, evolution, and molecular biology studies within the Andropogoneae. |
author2 |
CLAUDIA TEIXEIRA GUIMARAES, CNPMS. |
author_facet |
CLAUDIA TEIXEIRA GUIMARAES, CNPMS. GUIMARAES, C. T. HONEYCUTT, R. J. SILLS, G. R. SOBRAL, W. S. |
format |
Parte de livro |
topic_facet |
Mapeamento Mapping Biotehnology. Biotecnologia Cana de Açúcar DNA Genética. genetics sugarcane. |
author |
GUIMARAES, C. T. HONEYCUTT, R. J. SILLS, G. R. SOBRAL, W. S. |
author_sort |
GUIMARAES, C. T. |
title |
Genetic maps of Saccharum officinarum L. and Saccharum robustum Brandes & Jew ex grassl. |
title_short |
Genetic maps of Saccharum officinarum L. and Saccharum robustum Brandes & Jew ex grassl. |
title_full |
Genetic maps of Saccharum officinarum L. and Saccharum robustum Brandes & Jew ex grassl. |
title_fullStr |
Genetic maps of Saccharum officinarum L. and Saccharum robustum Brandes & Jew ex grassl. |
title_full_unstemmed |
Genetic maps of Saccharum officinarum L. and Saccharum robustum Brandes & Jew ex grassl. |
title_sort |
genetic maps of saccharum officinarum l. and saccharum robustum brandes & jew ex grassl. |
publishDate |
2000-03-14 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/483496 |
work_keys_str_mv |
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_version_ |
1756017437780738048 |