Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum Rifai.

Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum Rifai.

The isolation of genetic markers, like drug resistance and auxotrophy, is a laborious but important step in genetic research. The isolation of auxotrophic mutants of Trichoderma harzianum using the filtration enrichment technique was more effective than using the total isolation technique. Most of 12 auxotrophic mutants exhibited similar growth rate and higher sporulation when compared with the wild type, but only two mutants (TWS-410 and TW5-523) could grow in 500ug/L of benomyl.

Saved in:
Bibliographic Details
Main Authors: CASSIOLATO, A. M. R., MELO, I. S. de
Other Authors: EMBRAPA-CNPMA; ITAMAR SOARES DE MELO, CNPMA.
Format: Artigo de periódico biblioteca
Language:English
eng
Published: 1999-09-08
Subjects:Techniques, Culture medium, benomyl, biological control, fungi, mutants, plant pathogens,
Online Access:http://www.alice.cnptia.embrapa.br/alice/handle/doc/13152
Tags: Add Tag
No Tags, Be the first to tag this record!
id dig-alice-doc-13152
record_format koha
spelling dig-alice-doc-131522019-07-18T00:44:20Z Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum Rifai. CASSIOLATO, A. M. R. MELO, I. S. de EMBRAPA-CNPMA; ITAMAR SOARES DE MELO, CNPMA. Techniques Culture medium benomyl biological control fungi mutants plant pathogens The isolation of genetic markers, like drug resistance and auxotrophy, is a laborious but important step in genetic research. The isolation of auxotrophic mutants of Trichoderma harzianum using the filtration enrichment technique was more effective than using the total isolation technique. Most of 12 auxotrophic mutants exhibited similar growth rate and higher sporulation when compared with the wild type, but only two mutants (TWS-410 and TW5-523) could grow in 500ug/L of benomyl. 2019-07-18T00:44:14Z 2019-07-18T00:44:14Z 1999-09-08 1999 2019-12-12T11:11:11Z Artigo de periódico Revista de Microbiologia, v.30, p.43-46, 1999. http://www.alice.cnptia.embrapa.br/alice/handle/doc/13152 en eng openAccess p. 43-46
institution EMBRAPA
collection DSpace
country Brasil
countrycode BR
component Bibliográfico
access En linea
databasecode dig-alice
tag biblioteca
region America del Sur
libraryname Sistema de bibliotecas de EMBRAPA
language English
eng
topic Techniques
Culture medium
benomyl
biological control
fungi
mutants
plant pathogens
Techniques
Culture medium
benomyl
biological control
fungi
mutants
plant pathogens
spellingShingle Techniques
Culture medium
benomyl
biological control
fungi
mutants
plant pathogens
Techniques
Culture medium
benomyl
biological control
fungi
mutants
plant pathogens
CASSIOLATO, A. M. R.
MELO, I. S. de
Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum Rifai.
description The isolation of genetic markers, like drug resistance and auxotrophy, is a laborious but important step in genetic research. The isolation of auxotrophic mutants of Trichoderma harzianum using the filtration enrichment technique was more effective than using the total isolation technique. Most of 12 auxotrophic mutants exhibited similar growth rate and higher sporulation when compared with the wild type, but only two mutants (TWS-410 and TW5-523) could grow in 500ug/L of benomyl.
author2 EMBRAPA-CNPMA; ITAMAR SOARES DE MELO, CNPMA.
author_facet EMBRAPA-CNPMA; ITAMAR SOARES DE MELO, CNPMA.
CASSIOLATO, A. M. R.
MELO, I. S. de
format Artigo de periódico
topic_facet Techniques
Culture medium
benomyl
biological control
fungi
mutants
plant pathogens
author CASSIOLATO, A. M. R.
MELO, I. S. de
author_sort CASSIOLATO, A. M. R.
title Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum Rifai.
title_short Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum Rifai.
title_full Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum Rifai.
title_fullStr Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum Rifai.
title_full_unstemmed Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum Rifai.
title_sort filtration enrichment method for isolation of auxotrophic mutants of trichoderma harzianum rifai.
publishDate 1999-09-08
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/13152
work_keys_str_mv AT cassiolatoamr filtrationenrichmentmethodforisolationofauxotrophicmutantsoftrichodermaharzianumrifai
AT meloisde filtrationenrichmentmethodforisolationofauxotrophicmutantsoftrichodermaharzianumrifai
_version_ 1756026067838042112

Similar Items