Isolation and identification of broad-spectrum antagonist bacteria against pathogenic fungi of maize crop.

Fungal diseases may cause significant damage to crops worldwide, generating yield losses, poor grain quality, and health risks to humans and animals. Biological control using antagonistic bacteria offers innovative solutions for sustainable management aiming at plant protection. However, beneficial plant-microorganism interactions are particular, and few antagonists with broad-spectrum activity have been reported. In this work, two bacteria isolated from sorghum seeds were identified by partial sequencing of the 16S rRNA gene and tested in vitro for their capacity to control six important pathogenic fungi of maize: Fusarium verticillioides, Macrophomina phaseolina, Stenocarpella sp., Fusarium graminearum, Colletotrichum graminicola, and Bipolaris sp. The molecular identification revealed that the bacterial isolates belong to the genera Bacillus (strain LIS05) and Paenibacillus (strain LIS04). Both bacterial isolates inhibited the growth of all six phytopathogens by at least 49%. The isolate LIS05 showed the most significant antagonistic potential against the fungal pathogens tested, at an average of 73% inhibition. The highest antagonist activity (86.1% inhibition) was observed in the confrontation test between the isolate LIS05 and C. graminicola. In addition to the mycelial growth inhibition, the isolate LIS04 blocked the production of dark pigments by Bipolaris sp. This study showed that LIS05 and LIS04 are promising alternatives for developing integrated management strategies to control fungal diseases in maize and sorghum

Bibliographic Details

Main Authors:	DINIZ, G. de F. D., COTA, L. V., FIGUEIREDO, J. E. F., LANA, U. G. de P., MARINS, M. S., SILVA, F. C., SANTOS, V. L. dos, OLIVEIRA-PAIVA, C. A.
Other Authors:	GISELE DE FÁTIMA DIAS DINIZ; LUCIANO VIANA COTA, CNPMS; JOSE EDSON FONTES FIGUEIREDO, CNPMS; UBIRACI GOMES DE PAULA LANA, CNPMS; MIKAELY SOUSA MARINS, UNIVERSIDADE FEDERAL DE LAVRAS; FELIPE CAMPOS SILVA, UNIVERSIDADE FEDERAL DE SÃO JOÃO DEL-REI; VERA LÚCIA DOS SANTOS, UNIVERSIDADE FEDERAL DE MINAS GERAIS; CHRISTIANE ABREU DE OLIVEIRA PAIVA, CNPMS.
Format:	Artigo de periódico biblioteca
Language:	Ingles English
Published:	2024-02-26
Subjects:	Fungo fitopatogênico, Biocontrole, Milho, Zea Mays, Fungo, Paenibacillus, Bacillus (bacteria),
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1162254
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