CRISPR technology towards genome editing of the perennial and semi-perennial crops citrus, coffee and sugarcane.
Gene editing technologies have opened up the possibility of manipulating the genome of any organism in a predicted way. CRISPR technology is the most used genome editing tool and, in agriculture, it has allowed the expansion of possibilities in plant biotechnology, such as gene knockout or knock-in, transcriptional regulation, epigenetic modification, base editing, RNA editing, prime editing, and nucleic acid probing or detection. This technology mostly depends on in vitro tissue culture and genetic transformation/transfection protocols, which sometimes become the major challenges for its application in different crops. Agrobacterium-mediated transformation, biolistics, plasmid or RNP (ribonucleoprotein) transfection of protoplasts are some of the commonly used CRISPR delivery methods, but they depend on the genotype and target gene for efficient editing. The choice of the CRISPR system (Cas9, Cas12), CRISPR mechanism (plasmid or RNP) and transfection technique (Agrobacterium spp., PEG solution, lipofection) directly impacts the transformation efficiency and/or editing rate. Besides, CRISPR/Cas technology has made countries rethink regulatory frameworks concerning genetically modified organisms and flexibilize regulatory obstacles for edited plants. Here we present an overview of the state-of-the-art of CRISPR technology applied to three important crops worldwide (citrus, coffee and sugarcane), considering the biological, methodological, and regulatory aspects of its application. In addition, we provide perspectives on recently developed CRISPR tools and promising applications for each of these crops, thus highlighting the usefulness of gene editing to develop novel cultivars.
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Format: | Artigo de periódico biblioteca |
Language: | Ingles English |
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2024-01-08
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Subjects: | Genome, Perennials, Citrus, Coffee beans, Sugarcane, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1160555 https://doi.org/10.3389/fpls.2023.1331258 |
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dig-alice-doc-11605552024-01-08T19:32:43Z CRISPR technology towards genome editing of the perennial and semi-perennial crops citrus, coffee and sugarcane. PRADO, G. S. ROCHA, D. C. SANTOS, L. N. dos CONTILIANI, D. F. NOBILE, P. M. MARTINATI-SCHENK, J. C. PADILHA, L. MALUF, M. P. LUBINI, G. PEREIRA, T. C. MONTEIRO-VITORELLO, C. B. CRESTE, S. BOSCARIOL-CAMARGO, R. L. TAKITA, M. A. CRISTOFANI-YALY, M. SOUZA, A. A. de GUILHERME SOUZA PRADO, INSTITUTO AGRONÔMICO; DHIÔVANNA CORRÊIA ROCHA, UNIVERSIDADE ESTADUAL DE CAMPINAS; LUCAS NASCIMENTO DOS SANTOS, INSTITUTO AGRONÔMICO; DANYEL FERNANDES CONTILIANI, INSTITUTO AGRONÔMICO; PAULA MACEDO NOBILE, INSTITUTO AGRONÔMICO; JULIANA CAMARGO MARTINATI-SCHENK, INSTITUTO AGRONÔMICO; LILIAN PADILHA, CNPCa; MIRIAN PEREZ MALUF, CNPCa; GREICE LUBINI, INSTITUTO AGRONÔMICO; TIAGO CAMPOS PEREIRA, UNIVERSIDADE DE SÃO PAULO; CLAUDIA BARROS MONTEIRO-VITORELLO, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; SILVANA CRESTE, INSTITUTO AGRONÔMICO; RAQUEL LUCIANA BOSCARIOL-CAMARGO, INSTITUTO AGRONÔMICO; MARCO AURÉLIO TAKITA, INSTITUTO AGRONÔMICO; MARIÂNGELA CRISTOFANI-YALY, INSTITUTO AGRONÔMICO; ALESSANDRA ALVES DE SOUZA, INSTITUTO AGRONÔMICO. Genome Perennials Citrus Coffee beans Sugarcane Gene editing technologies have opened up the possibility of manipulating the genome of any organism in a predicted way. CRISPR technology is the most used genome editing tool and, in agriculture, it has allowed the expansion of possibilities in plant biotechnology, such as gene knockout or knock-in, transcriptional regulation, epigenetic modification, base editing, RNA editing, prime editing, and nucleic acid probing or detection. This technology mostly depends on in vitro tissue culture and genetic transformation/transfection protocols, which sometimes become the major challenges for its application in different crops. Agrobacterium-mediated transformation, biolistics, plasmid or RNP (ribonucleoprotein) transfection of protoplasts are some of the commonly used CRISPR delivery methods, but they depend on the genotype and target gene for efficient editing. The choice of the CRISPR system (Cas9, Cas12), CRISPR mechanism (plasmid or RNP) and transfection technique (Agrobacterium spp., PEG solution, lipofection) directly impacts the transformation efficiency and/or editing rate. Besides, CRISPR/Cas technology has made countries rethink regulatory frameworks concerning genetically modified organisms and flexibilize regulatory obstacles for edited plants. Here we present an overview of the state-of-the-art of CRISPR technology applied to three important crops worldwide (citrus, coffee and sugarcane), considering the biological, methodological, and regulatory aspects of its application. In addition, we provide perspectives on recently developed CRISPR tools and promising applications for each of these crops, thus highlighting the usefulness of gene editing to develop novel cultivars. 2024-01-08T19:32:43Z 2024-01-08T19:32:43Z 2024-01-08 2023 Artigo de periódico Frontiers in Plant Science, v. 14, article 1331258, 2023. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1160555 https://doi.org/10.3389/fpls.2023.1331258 Ingles en openAccess |
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Genome Perennials Citrus Coffee beans Sugarcane Genome Perennials Citrus Coffee beans Sugarcane |
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Genome Perennials Citrus Coffee beans Sugarcane Genome Perennials Citrus Coffee beans Sugarcane PRADO, G. S. ROCHA, D. C. SANTOS, L. N. dos CONTILIANI, D. F. NOBILE, P. M. MARTINATI-SCHENK, J. C. PADILHA, L. MALUF, M. P. LUBINI, G. PEREIRA, T. C. MONTEIRO-VITORELLO, C. B. CRESTE, S. BOSCARIOL-CAMARGO, R. L. TAKITA, M. A. CRISTOFANI-YALY, M. SOUZA, A. A. de CRISPR technology towards genome editing of the perennial and semi-perennial crops citrus, coffee and sugarcane. |
description |
Gene editing technologies have opened up the possibility of manipulating the genome of any organism in a predicted way. CRISPR technology is the most used genome editing tool and, in agriculture, it has allowed the expansion of possibilities in plant biotechnology, such as gene knockout or knock-in, transcriptional regulation, epigenetic modification, base editing, RNA editing, prime editing, and nucleic acid probing or detection. This technology mostly depends on in vitro tissue culture and genetic transformation/transfection protocols, which sometimes become the major challenges for its application in different crops. Agrobacterium-mediated transformation, biolistics, plasmid or RNP (ribonucleoprotein) transfection of protoplasts are some of the commonly used CRISPR delivery methods, but they depend on the genotype and target gene for efficient editing. The choice of the CRISPR system (Cas9, Cas12), CRISPR mechanism (plasmid or RNP) and transfection technique (Agrobacterium spp., PEG solution, lipofection) directly impacts the transformation efficiency and/or editing rate. Besides, CRISPR/Cas technology has made countries rethink regulatory frameworks concerning genetically modified organisms and flexibilize regulatory obstacles for edited plants. Here we present an overview of the state-of-the-art of CRISPR technology applied to three important crops worldwide (citrus, coffee and sugarcane), considering the biological, methodological, and regulatory aspects of its application. In addition, we provide perspectives on recently developed CRISPR tools and promising applications for each of these crops, thus highlighting the usefulness of gene editing to develop novel cultivars. |
author2 |
GUILHERME SOUZA PRADO, INSTITUTO AGRONÔMICO; DHIÔVANNA CORRÊIA ROCHA, UNIVERSIDADE ESTADUAL DE CAMPINAS; LUCAS NASCIMENTO DOS SANTOS, INSTITUTO AGRONÔMICO; DANYEL FERNANDES CONTILIANI, INSTITUTO AGRONÔMICO; PAULA MACEDO NOBILE, INSTITUTO AGRONÔMICO; JULIANA CAMARGO MARTINATI-SCHENK, INSTITUTO AGRONÔMICO; LILIAN PADILHA, CNPCa; MIRIAN PEREZ MALUF, CNPCa; GREICE LUBINI, INSTITUTO AGRONÔMICO; TIAGO CAMPOS PEREIRA, UNIVERSIDADE DE SÃO PAULO; CLAUDIA BARROS MONTEIRO-VITORELLO, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; SILVANA CRESTE, INSTITUTO AGRONÔMICO; RAQUEL LUCIANA BOSCARIOL-CAMARGO, INSTITUTO AGRONÔMICO; MARCO AURÉLIO TAKITA, INSTITUTO AGRONÔMICO; MARIÂNGELA CRISTOFANI-YALY, INSTITUTO AGRONÔMICO; ALESSANDRA ALVES DE SOUZA, INSTITUTO AGRONÔMICO. |
author_facet |
GUILHERME SOUZA PRADO, INSTITUTO AGRONÔMICO; DHIÔVANNA CORRÊIA ROCHA, UNIVERSIDADE ESTADUAL DE CAMPINAS; LUCAS NASCIMENTO DOS SANTOS, INSTITUTO AGRONÔMICO; DANYEL FERNANDES CONTILIANI, INSTITUTO AGRONÔMICO; PAULA MACEDO NOBILE, INSTITUTO AGRONÔMICO; JULIANA CAMARGO MARTINATI-SCHENK, INSTITUTO AGRONÔMICO; LILIAN PADILHA, CNPCa; MIRIAN PEREZ MALUF, CNPCa; GREICE LUBINI, INSTITUTO AGRONÔMICO; TIAGO CAMPOS PEREIRA, UNIVERSIDADE DE SÃO PAULO; CLAUDIA BARROS MONTEIRO-VITORELLO, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; SILVANA CRESTE, INSTITUTO AGRONÔMICO; RAQUEL LUCIANA BOSCARIOL-CAMARGO, INSTITUTO AGRONÔMICO; MARCO AURÉLIO TAKITA, INSTITUTO AGRONÔMICO; MARIÂNGELA CRISTOFANI-YALY, INSTITUTO AGRONÔMICO; ALESSANDRA ALVES DE SOUZA, INSTITUTO AGRONÔMICO. PRADO, G. S. ROCHA, D. C. SANTOS, L. N. dos CONTILIANI, D. F. NOBILE, P. M. MARTINATI-SCHENK, J. C. PADILHA, L. MALUF, M. P. LUBINI, G. PEREIRA, T. C. MONTEIRO-VITORELLO, C. B. CRESTE, S. BOSCARIOL-CAMARGO, R. L. TAKITA, M. A. CRISTOFANI-YALY, M. SOUZA, A. A. de |
format |
Artigo de periódico |
topic_facet |
Genome Perennials Citrus Coffee beans Sugarcane |
author |
PRADO, G. S. ROCHA, D. C. SANTOS, L. N. dos CONTILIANI, D. F. NOBILE, P. M. MARTINATI-SCHENK, J. C. PADILHA, L. MALUF, M. P. LUBINI, G. PEREIRA, T. C. MONTEIRO-VITORELLO, C. B. CRESTE, S. BOSCARIOL-CAMARGO, R. L. TAKITA, M. A. CRISTOFANI-YALY, M. SOUZA, A. A. de |
author_sort |
PRADO, G. S. |
title |
CRISPR technology towards genome editing of the perennial and semi-perennial crops citrus, coffee and sugarcane. |
title_short |
CRISPR technology towards genome editing of the perennial and semi-perennial crops citrus, coffee and sugarcane. |
title_full |
CRISPR technology towards genome editing of the perennial and semi-perennial crops citrus, coffee and sugarcane. |
title_fullStr |
CRISPR technology towards genome editing of the perennial and semi-perennial crops citrus, coffee and sugarcane. |
title_full_unstemmed |
CRISPR technology towards genome editing of the perennial and semi-perennial crops citrus, coffee and sugarcane. |
title_sort |
crispr technology towards genome editing of the perennial and semi-perennial crops citrus, coffee and sugarcane. |
publishDate |
2024-01-08 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1160555 https://doi.org/10.3389/fpls.2023.1331258 |
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