Depleção do quadro de leitura upstream como nova estratégia para manipular a tradução de gmpr10 usando crispr/cas9 para aumentar a tolerância da soja a fitonematoides.
In this study, we identified two uORFs in the 5'-UTR sequence of this gene. The entire 5' leader sequence was amplified, cloned, and site-directed mutated to delete its uORFs start codons (-ATG). The validation of these predicted uORFs was carried out in transformed Nicotiana benthamiana protoplasts using a dual-luciferase reporter vector, in which it is possible to analyze the effect of mutated and non-mutated uORFs by dividing the expression of luciferase by that of Renilla-luciferase (LUC/REN).
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Main Authors: | COSTA, L. S. de L., ALVES, N. S. de F., PINTO, C. E. M., FLORENTINO, L. H., MELO, B. P. de, MOREIRA, V. J. V., SÁ, M. E. L. de, ARRAES, F. B. M., RECH FILHO, E. L., MORGANTE, C. V., SA, M. F. G. de |
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Other Authors: | LORENA SOUSA DE LOIOLA COSTA, Bolsista - Campus Universitário Darcy Ribeiro, University of Brasilia, DF |
Format: | Resumo em anais e proceedings biblioteca |
Language: | Ingles English |
Published: |
2023-11-17
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Subjects: | UORF, Edição do genoma, Nematóide das galhas, Glycine Max, Meloidogyne Incognita, Soja, Root-knot nematodes, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1158490 |
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