Enhanced mercury phytoremediation by Pseudomonodictys pantanalensis sp. nov. A73 and Westerdykella aquatica P71.
Mercury is a non-essential and toxic metal that induces toxicity in most organisms, but endophytic fungi can develop survival strategies to tolerate and respond to metal contaminants and other environmental stressors. The present study demonstrated the potential of mercury-resistant endophytic fungi in phytoremediation. We examined the functional traits involved in plant growth promotion, phytotoxicity mitigation, and mercury phytoremediation in seven fungi strains. The endophytic isolates synthesized the phytohormone indole-3-acetic acid, secreted siderophores, and solubilized phosphate in vitro. Inoculation of maize (Zea mays) plants with endophytes increased plant growth attributes by up to 76.25%. The endophytic fungi stimulated mercury uptake from the substrate and promoted its accumulation in plant tissues (t test, p<0.05), preferentially in the roots, which thereby mitigated the impacts of metal phytotoxicity. Westerdykella aquatica P71 and the newly identifed species Pseudomonodictys pantanalensis nov. A73 were the isolates that presented the best phytoremediation potential. Assembling and annotation of P. pantanalensis A73 and W. aquatica P71 genomes resulted in genome sizes of 45.7 and 31.8 Mb that encoded 17,774 and 11,240 protein-coding genes, respectively. Some clusters of genes detected were involved in the synthesis of secondary metabolites such as dimethylcoprogen (NRPS) and melanin (T1PKS), which are metal chelators with antioxidant activity; mercury resistance (merA and merR1); oxidative stress (PRX1 and TRX1); and plant growth promotion (trpS and iscU). Therefore, both fungi species are potential tools for the bioremediation of mercury-contaminated soils due to their ability to reduce phytotoxicity and assist phytoremediation.
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Format: | Artigo de periódico biblioteca |
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2023-05-09
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Subjects: | Genome draft, Zea Mays, Milho, Endophytes, Secondary metabolites, Bioremediation, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1153593 https://doi.org/10.1007/s42770-023-00924-4 |
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dig-alice-doc-11535932023-08-02T10:23:42Z Enhanced mercury phytoremediation by Pseudomonodictys pantanalensis sp. nov. A73 and Westerdykella aquatica P71. SENABIO, J. A. PEREIRA, F. de C. PIETRO-SOUZA, W. SOUSA, T. F. SILVA, G. F. da SOARES, M. A. JAQUELINE ALVES SENABIO, UNIVERSIDADE FEDERAL DE MATO GROSSO; FELIPE DE CAMPOS PEREIRA, UNIVERSIDADE FEDERAL DE MATO GROSSO; WILLIAM PIETRO-SOUZA, UNIVERSIDADE FEDERAL DE MATO GROSSO; THIAGO FERNANDES SOUSA, UNIVERSIDADE DE VIÇOSA; GILVAN FERREIRA DA SILVA, CPAA; MARCOS ANTÔNIO SOARES, UNIVERSIDADE FEDERAL DE MATO GROSSO. Genome draft Zea Mays Milho Endophytes Secondary metabolites Bioremediation Mercury is a non-essential and toxic metal that induces toxicity in most organisms, but endophytic fungi can develop survival strategies to tolerate and respond to metal contaminants and other environmental stressors. The present study demonstrated the potential of mercury-resistant endophytic fungi in phytoremediation. We examined the functional traits involved in plant growth promotion, phytotoxicity mitigation, and mercury phytoremediation in seven fungi strains. The endophytic isolates synthesized the phytohormone indole-3-acetic acid, secreted siderophores, and solubilized phosphate in vitro. Inoculation of maize (Zea mays) plants with endophytes increased plant growth attributes by up to 76.25%. The endophytic fungi stimulated mercury uptake from the substrate and promoted its accumulation in plant tissues (t test, p<0.05), preferentially in the roots, which thereby mitigated the impacts of metal phytotoxicity. Westerdykella aquatica P71 and the newly identifed species Pseudomonodictys pantanalensis nov. A73 were the isolates that presented the best phytoremediation potential. Assembling and annotation of P. pantanalensis A73 and W. aquatica P71 genomes resulted in genome sizes of 45.7 and 31.8 Mb that encoded 17,774 and 11,240 protein-coding genes, respectively. Some clusters of genes detected were involved in the synthesis of secondary metabolites such as dimethylcoprogen (NRPS) and melanin (T1PKS), which are metal chelators with antioxidant activity; mercury resistance (merA and merR1); oxidative stress (PRX1 and TRX1); and plant growth promotion (trpS and iscU). Therefore, both fungi species are potential tools for the bioremediation of mercury-contaminated soils due to their ability to reduce phytotoxicity and assist phytoremediation. 2023-08-02T10:23:42Z 2023-08-02T10:23:42Z 2023-05-09 2023 Artigo de periódico Brazilian Journal of Microbiology, v. 54, n. 2, p. 949-964, jun. 2023. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1153593 https://doi.org/10.1007/s42770-023-00924-4 Ingles en openAccess |
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Genome draft Zea Mays Milho Endophytes Secondary metabolites Bioremediation Genome draft Zea Mays Milho Endophytes Secondary metabolites Bioremediation |
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Genome draft Zea Mays Milho Endophytes Secondary metabolites Bioremediation Genome draft Zea Mays Milho Endophytes Secondary metabolites Bioremediation SENABIO, J. A. PEREIRA, F. de C. PIETRO-SOUZA, W. SOUSA, T. F. SILVA, G. F. da SOARES, M. A. Enhanced mercury phytoremediation by Pseudomonodictys pantanalensis sp. nov. A73 and Westerdykella aquatica P71. |
description |
Mercury is a non-essential and toxic metal that induces toxicity in most organisms, but endophytic fungi can develop survival strategies to tolerate and respond to metal contaminants and other environmental stressors. The present study demonstrated the potential of mercury-resistant endophytic fungi in phytoremediation. We examined the functional traits involved in plant growth promotion, phytotoxicity mitigation, and mercury phytoremediation in seven fungi strains. The endophytic isolates synthesized the phytohormone indole-3-acetic acid, secreted siderophores, and solubilized phosphate in vitro. Inoculation of maize (Zea mays) plants with endophytes increased plant growth attributes by up to 76.25%. The endophytic fungi stimulated mercury uptake from the substrate and promoted its accumulation in plant tissues (t test, p<0.05), preferentially in the roots, which thereby mitigated the impacts of metal phytotoxicity. Westerdykella aquatica P71 and the newly identifed species Pseudomonodictys pantanalensis nov. A73 were the isolates that presented the best phytoremediation potential. Assembling and annotation of P. pantanalensis A73 and W. aquatica P71 genomes resulted in genome sizes of 45.7 and 31.8 Mb that encoded 17,774 and 11,240 protein-coding genes, respectively. Some clusters of genes detected were involved in the synthesis of secondary metabolites such as dimethylcoprogen (NRPS) and melanin (T1PKS), which are metal chelators with antioxidant activity; mercury resistance (merA and merR1); oxidative stress (PRX1 and TRX1); and plant growth promotion (trpS and iscU). Therefore, both fungi species are potential tools for the bioremediation of mercury-contaminated soils due to their ability to reduce phytotoxicity and assist phytoremediation. |
author2 |
JAQUELINE ALVES SENABIO, UNIVERSIDADE FEDERAL DE MATO GROSSO; FELIPE DE CAMPOS PEREIRA, UNIVERSIDADE FEDERAL DE MATO GROSSO; WILLIAM PIETRO-SOUZA, UNIVERSIDADE FEDERAL DE MATO GROSSO; THIAGO FERNANDES SOUSA, UNIVERSIDADE DE VIÇOSA; GILVAN FERREIRA DA SILVA, CPAA; MARCOS ANTÔNIO SOARES, UNIVERSIDADE FEDERAL DE MATO GROSSO. |
author_facet |
JAQUELINE ALVES SENABIO, UNIVERSIDADE FEDERAL DE MATO GROSSO; FELIPE DE CAMPOS PEREIRA, UNIVERSIDADE FEDERAL DE MATO GROSSO; WILLIAM PIETRO-SOUZA, UNIVERSIDADE FEDERAL DE MATO GROSSO; THIAGO FERNANDES SOUSA, UNIVERSIDADE DE VIÇOSA; GILVAN FERREIRA DA SILVA, CPAA; MARCOS ANTÔNIO SOARES, UNIVERSIDADE FEDERAL DE MATO GROSSO. SENABIO, J. A. PEREIRA, F. de C. PIETRO-SOUZA, W. SOUSA, T. F. SILVA, G. F. da SOARES, M. A. |
format |
Artigo de periódico |
topic_facet |
Genome draft Zea Mays Milho Endophytes Secondary metabolites Bioremediation |
author |
SENABIO, J. A. PEREIRA, F. de C. PIETRO-SOUZA, W. SOUSA, T. F. SILVA, G. F. da SOARES, M. A. |
author_sort |
SENABIO, J. A. |
title |
Enhanced mercury phytoremediation by Pseudomonodictys pantanalensis sp. nov. A73 and Westerdykella aquatica P71. |
title_short |
Enhanced mercury phytoremediation by Pseudomonodictys pantanalensis sp. nov. A73 and Westerdykella aquatica P71. |
title_full |
Enhanced mercury phytoremediation by Pseudomonodictys pantanalensis sp. nov. A73 and Westerdykella aquatica P71. |
title_fullStr |
Enhanced mercury phytoremediation by Pseudomonodictys pantanalensis sp. nov. A73 and Westerdykella aquatica P71. |
title_full_unstemmed |
Enhanced mercury phytoremediation by Pseudomonodictys pantanalensis sp. nov. A73 and Westerdykella aquatica P71. |
title_sort |
enhanced mercury phytoremediation by pseudomonodictys pantanalensis sp. nov. a73 and westerdykella aquatica p71. |
publishDate |
2023-05-09 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1153593 https://doi.org/10.1007/s42770-023-00924-4 |
work_keys_str_mv |
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