In vitro antiviral efect of ethanolic extracts from Azadirachta indica and Melia azedarach against goat lentivirus in colostrum and milk.

Abstract: This study aimed to evaluate, in vitro, the use of leaf extracts of Azadirachta indica (A. indica) and Melia azedarach (M. azedarach) as antivirals against caprine lentivirus (CLV) in colostrum and milk of goat nannies. These were collected from eight individuals and infected with the standard strain of CLV. Samples were then subdivided into aliquots and treated with 150 µg/mL of crude extract, and with ethyl acetate and methanol fractions for 30, 60, and 90 min. Next, somatic cells from colostrum and milk were co-cultured with cells from the ovine third eyelid. After this step, viral titers of the supernatants collected from treatments with greater efficacy in co-culture were assessed. The organic ethyl acetate fractions of both plants at 90 min possibly inhibited the viral activity of CLV by up to a thousandfold in colostrum. In milk, this inhibition was up to 800 times for the respective Meliaceae. In conclusion, the ethanolic fraction of ethyl acetate from both plants demonstrated efficacy against CLV in samples from colostrum and milk when subjected to treatment, which was more effective in colostrum.

Bibliographic Details

Main Authors:	SOUSA, A. L. M. de, PINHEIRO, R. R., ARAUJO, J. F., PEIXOTO, R. M., AZEVEDO, D. A. A. de, LIMA, A. M. C., CANUTO, K. M., RIBEIRO, P. R. V., SOUZA, A. S. de Q., SOUZA, S. C. R., AMORIM, S. L. de, AMARAL, G. P., SOUZA, V. de, MORAIS, S. M. de, ANDRIOLI, A., TEIXEIRA, M. F. da S.
Other Authors:	ANA LIDIA MADEIRA DE SOUSA, Laboratory of Virology (LABOVIR), State University of Ceará (UECE); RAYMUNDO RIZALDO PINHEIRO, CNPC; JUSCILANIA FURTADO ARAUJO, University Center INTA (UNINTA), Tianguá, CE, Brazil; RENATO MESQUITA PEIXOTO, Vale do Salgado University Center (UNIVS) - Icó, CE, Brazil; Terra Nordeste College (FATENE) - Caucaia, CE, Brazil; DALVA ALANA ARAGAO DE AZEVEDO; ANA MILENA CESAR LIMA, National Council for Scientific and Technological Development (DCR-CNPq/FUNCAP), Embrapa; KIRLEY MARQUES CANUTO, CNPAT; PAULO RICELI VASCONCELOS RIBEIRO, CNPAT; ANA SHEILA DE QUEIROZ SOUZA; SAMARA CRISTINA ROCHA SOUZA; SARA LUCENA DE AMORIM; GABRIEL PAULA AMARAL, State University of Acaraú Valley - Sobral, CE, Brazil; VIVIANE DE SOUZA, CNPC; SELENE MAIA DE MORAIS, Laboratory of Chemistry and Natural Products (LQPN), Ceará State University, Fortaleza, CE, Brazil; ALICE ANDRIOLI PINHEIRO, CNPC; MARIA FATIMA DA SILVA TEIXEIRA, Laboratory of Virology (LABOVIR), State University of Ceará (UECE), Fortaleza, CE, Brazil.
Format:	Artigo de periódico biblioteca
Language:	Ingles English
Published:	2023-03-22
Subjects:	Ethanolic extract, Antiviral plants, Composto bioativo, Extrato de plantas, SRLV, CAEV, CLV, Caprine Lentivirus, Planta Medicinal, Melia Azedarach, Azadirachta Indica, Nim, Extrato Vegetal, Fitoterapia, Plant extracts, Medicinal plants, Antiviral properties, Phytotherapy, Sheep diseases, Goat diseases,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1152602 https://doi.org/10.1038/s41598-023-31455-5
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