Acclimatization of coffee seedlings obtained from zygotic embryos of aged seeds.
Coffee seeds rapidly lose viability during storage, which hinders the development of vigorous seedlings for crop establishment. There are reports that seed endosperm is more sensitive to deterioration than embryos, which can be excised and cultivated in vitro. However, a substantial number of plants grown in vitro do not survive during transfer to a greenhouse or field environment. The objective of this study was to evaluate the acclimatization of coffee seedlings of cultivar Catuaí Amarelo IAC 62, developed from zygotic embryos obtained from aged seeds in different substrates and environments, for the production of well-developed seedlings suitable for planting. For this purpose, seedlings were obtained from the in vitro cultivation of embryos obtained from seeds of two quality levels: freshly harvested seeds and artificially aged seeds. Zygotic embryos were extracted from the seeds and cultivated in MS medium. At 60 days, the percentages of normal and abnormal seedlings and dead seeds were evaluated. The good-quality seedlings grown in vitro for 60 days were transplanted into two different substrates (Tropstrato and coconut fiber) and acclimatized in two environments (growth room and greenhouse with a misting system). The plants were evaluated for height, stem diameter, number of leaves, chlorophyll content, and growth rate. The greenhouse environment was better for seedling growth, possibly due to its higher sunlight and temperature. The best substrate was coconut fiber, as it ensured better development of plants from freshly harvested seeds and those from aged seeds. It is possible to develop healthy seedlings from seeds with low viability.
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Australian Journal of Crop Science, v. 16, n. 9, p.112-1134, 2022.
2023-01-11
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Subjects: | Coffea arabica var. arabica, In vitro culture, Greenhouses, Enzyme substrates, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1150877 |
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dig-alice-doc-11508772023-01-11T16:01:25Z Acclimatization of coffee seedlings obtained from zygotic embryos of aged seeds. VILELA, A. L. O. ROSA, S. D. V. F. da COELHO, S. V. B. PEREIRA, C. C. SOUZA, A. C. de RIBEIRO, F. A. S. ANA LUIZA OLIVEIRA VILELA, UNIVERSIDADE FEDERAL DE LAVRAS; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa; STEFÂNIA VILAS BOAS COELHO, UNIVERSIDADE FEDERAL DE LAVRAS; CRISTIANE CARVALHO PEREIRA, UNIVERSIDADE FEDERAL DE LAVRAS; ANA CRISTINA DE SOUZA, UNIVERSIDADE FEDERAL DE LAVRAS; FERNANDO AUGUSTO SALES RIBEIRO, UNIVERSIDADE FEDERAL DE LAVRAS. Coffea arabica var. arabica In vitro culture Greenhouses Enzyme substrates Coffee seeds rapidly lose viability during storage, which hinders the development of vigorous seedlings for crop establishment. There are reports that seed endosperm is more sensitive to deterioration than embryos, which can be excised and cultivated in vitro. However, a substantial number of plants grown in vitro do not survive during transfer to a greenhouse or field environment. The objective of this study was to evaluate the acclimatization of coffee seedlings of cultivar Catuaí Amarelo IAC 62, developed from zygotic embryos obtained from aged seeds in different substrates and environments, for the production of well-developed seedlings suitable for planting. For this purpose, seedlings were obtained from the in vitro cultivation of embryos obtained from seeds of two quality levels: freshly harvested seeds and artificially aged seeds. Zygotic embryos were extracted from the seeds and cultivated in MS medium. At 60 days, the percentages of normal and abnormal seedlings and dead seeds were evaluated. The good-quality seedlings grown in vitro for 60 days were transplanted into two different substrates (Tropstrato and coconut fiber) and acclimatized in two environments (growth room and greenhouse with a misting system). The plants were evaluated for height, stem diameter, number of leaves, chlorophyll content, and growth rate. The greenhouse environment was better for seedling growth, possibly due to its higher sunlight and temperature. The best substrate was coconut fiber, as it ensured better development of plants from freshly harvested seeds and those from aged seeds. It is possible to develop healthy seedlings from seeds with low viability. 2023-01-11T16:01:25Z 2023-01-11T16:01:25Z 2023-01-11 2022 Artigo de periódico http://www.alice.cnptia.embrapa.br/alice/handle/doc/1150877 Ingles en openAccess Australian Journal of Crop Science, v. 16, n. 9, p.112-1134, 2022. |
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Coffea arabica var. arabica In vitro culture Greenhouses Enzyme substrates Coffea arabica var. arabica In vitro culture Greenhouses Enzyme substrates |
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Coffea arabica var. arabica In vitro culture Greenhouses Enzyme substrates Coffea arabica var. arabica In vitro culture Greenhouses Enzyme substrates VILELA, A. L. O. ROSA, S. D. V. F. da COELHO, S. V. B. PEREIRA, C. C. SOUZA, A. C. de RIBEIRO, F. A. S. Acclimatization of coffee seedlings obtained from zygotic embryos of aged seeds. |
description |
Coffee seeds rapidly lose viability during storage, which hinders the development of vigorous seedlings for crop establishment. There are reports that seed endosperm is more sensitive to deterioration than embryos, which can be excised and cultivated in vitro. However, a substantial number of plants grown in vitro do not survive during transfer to a greenhouse or field environment. The objective of this study was to evaluate the acclimatization of coffee seedlings of cultivar Catuaí Amarelo IAC 62, developed from zygotic embryos obtained from aged seeds in different substrates and environments, for the production of well-developed seedlings suitable for planting. For this purpose, seedlings were obtained from the in vitro cultivation of embryos obtained from seeds of two quality levels: freshly harvested seeds and artificially aged seeds. Zygotic embryos were extracted from the seeds and cultivated in MS medium. At 60 days, the percentages of normal and abnormal seedlings and dead seeds were evaluated. The good-quality seedlings grown in vitro for 60 days were transplanted into two different substrates (Tropstrato and coconut fiber) and acclimatized in two environments (growth room and greenhouse with a misting system). The plants were evaluated for height, stem diameter, number of leaves, chlorophyll content, and growth rate. The greenhouse environment was better for seedling growth, possibly due to its higher sunlight and temperature. The best substrate was coconut fiber, as it ensured better development of plants from freshly harvested seeds and those from aged seeds. It is possible to develop healthy seedlings from seeds with low viability. |
author2 |
ANA LUIZA OLIVEIRA VILELA, UNIVERSIDADE FEDERAL DE LAVRAS; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa; STEFÂNIA VILAS BOAS COELHO, UNIVERSIDADE FEDERAL DE LAVRAS; CRISTIANE CARVALHO PEREIRA, UNIVERSIDADE FEDERAL DE LAVRAS; ANA CRISTINA DE SOUZA, UNIVERSIDADE FEDERAL DE LAVRAS; FERNANDO AUGUSTO SALES RIBEIRO, UNIVERSIDADE FEDERAL DE LAVRAS. |
author_facet |
ANA LUIZA OLIVEIRA VILELA, UNIVERSIDADE FEDERAL DE LAVRAS; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa; STEFÂNIA VILAS BOAS COELHO, UNIVERSIDADE FEDERAL DE LAVRAS; CRISTIANE CARVALHO PEREIRA, UNIVERSIDADE FEDERAL DE LAVRAS; ANA CRISTINA DE SOUZA, UNIVERSIDADE FEDERAL DE LAVRAS; FERNANDO AUGUSTO SALES RIBEIRO, UNIVERSIDADE FEDERAL DE LAVRAS. VILELA, A. L. O. ROSA, S. D. V. F. da COELHO, S. V. B. PEREIRA, C. C. SOUZA, A. C. de RIBEIRO, F. A. S. |
format |
Artigo de periódico |
topic_facet |
Coffea arabica var. arabica In vitro culture Greenhouses Enzyme substrates |
author |
VILELA, A. L. O. ROSA, S. D. V. F. da COELHO, S. V. B. PEREIRA, C. C. SOUZA, A. C. de RIBEIRO, F. A. S. |
author_sort |
VILELA, A. L. O. |
title |
Acclimatization of coffee seedlings obtained from zygotic embryos of aged seeds. |
title_short |
Acclimatization of coffee seedlings obtained from zygotic embryos of aged seeds. |
title_full |
Acclimatization of coffee seedlings obtained from zygotic embryos of aged seeds. |
title_fullStr |
Acclimatization of coffee seedlings obtained from zygotic embryos of aged seeds. |
title_full_unstemmed |
Acclimatization of coffee seedlings obtained from zygotic embryos of aged seeds. |
title_sort |
acclimatization of coffee seedlings obtained from zygotic embryos of aged seeds. |
publisher |
Australian Journal of Crop Science, v. 16, n. 9, p.112-1134, 2022. |
publishDate |
2023-01-11 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1150877 |
work_keys_str_mv |
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