Seed pretreatment for control of powdery mildew infection on purple ipe micropropagation.

Purple ipe (Handroanthus impetiginosus) is an important tree species in Cerrado biome conservation and very popular at the landscaping and urban afforestation. However, its micropropagation is affected by pathogens, such as Oidium sp. The aim this study was evaluate the efficiency of seed treatments in the control of powdery mildew of purple ipe obtained by micropropagation. The symptoms were observed during in vitro germination, a Koch?s postulates were performed for confirm the pathogenicity, colonization of the pathogen on the leaves was analyzed in optical and scanning microscopes and a scale to evaluate severity was proposed. Two experiments were realized to powdery mildew control using a completely randomized design, with 30 replicates. First experiment: Seeds were treated with ethanol (Et), chlorothalonil + thiophanate-methyl (C+TM), and sodium hypochlorite (NaOCl); second experiment: Seeds were treated with Et, NaOCl, C+TM, and neem oil. Disease severity and area under the disease progress curve (AUDPC) were assessed in both experiments. Disease symptoms and typical pathogen structures were observed, and the pathogenicity was confirmed. The disease severity was reduced by 30.78% in 1.5% neem oil for 10 min when compared with C+TM for 15 min. We conclude that neem oil can be a strategy sustainable for the control of powdery mildew in purple ipe in tissue culture.

Bibliographic Details

Main Authors:	MAMEDES, T. C., CHAIBUB, A. A., SOUSA, K. C. I., FARIA, M. T., GONÇALVES, L. de A., FILIPPI, M. C. C. de, ARAÚJO, L. G. de, SIBOV, S. T.
Other Authors:	TALITA CRISTINA MAMEDES, UNIVERSIDADE FEDERAL DE GOIÁS; AMANDA ABDALLAH CHAIBUB, UNIVERSIDADE FEDERAL DE GOIÁS; KELLEN CRISTHINA INÁCIO SOUSA, UNIVERSIDADE FEDERAL DE GOIÁS; MARIA TEREZA FARIA, UNIVERSIDADE FEDERAL DE GOIÁS; LETÍCIA DE ALMEIDA GONÇALVES, UNIVERSIDADE FEDERAL DE GOIÁS; MARTA CRISTINA CORSI DE FILIPPI, CNPAF; LEILA GARCÊS DE ARAÚJO, UNIVERSIDADE FEDERAL DE GOIÁS; SÉRGIO TADEU SIBOV, UNIVERSIDADE FEDERAL DE GOIÁS.
Format:	Artigo de periódico biblioteca
Language:	Ingles English
Published:	Ornamental Horticulture, v. 28, n. 2, p. 193-201, 2022. 2022-11-04
Subjects:	Controle alternativo, Cultura de Tecido, Fungicida, Nim, Ipê Roxo, Handroanthus impetiginosus, Fungicides, Neem oil, Tissue culture,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1148054
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