Are stomatal area and stomatal density reliable traits for identification of doubled haploids in maize?

To meet the demands of the production chain, breeding programs need to reduce the time for selection and development of higher yielding maize genotypes. This involves application of new strategies, such as the use of the doubled haploid lines. We evaluated the use of stomatal area and density in distinguishing doubled haploids (DH) from false positive (FP) plants derived from nine tropical source populations. We selected four DH plants and two FP plants for identification of DH through leaf anatomy in the populations, in each group. Data on stomatal area and density were considered in a completely randomized model in a factorial scheme (populations and genotypes) and analyzed by restricted maximum likelihood. There were statistically significant effects of populations and genotypes for both traits. For stomatal density, the population by genotype interaction was not verified. Stomatal density proved to be efficient for distinguishing DH and FP for use in selection of doubled haploids.

Bibliographic Details

Main Authors:	RIBEIRO, M. R., TRINDADE, R. dos S., SOUZA, I. R. P. de, CARNEIRO, A. A., AZEVEDO, T. C. de, GUIMARAES, L. J. M., CHAVES, S. F. da S., DIAS, L. A. dos S.
Other Authors:	MARIANA RODRIGUES RIBEIRO, Universidade Federal de Viçosa; ROBERTO DOS SANTOS TRINDADE, CNPMS; ISABEL REGINA PRAZERES DE SOUZA, CNPMS; ANDREA ALMEIDA CARNEIRO, CNPMS; TÁCILA CRISTINA DE AZEVEDO; LAURO JOSE MOREIRA GUIMARAES, CNPMS; SAULO FABRÍCIO DA SILVA CHAVES, Universidade Federal de Viçosa; LUIZ ANTÔNIO DOS SANTOS DIAS, Universidade Federal de Viçosa.
Format:	Artigo de periódico biblioteca
Language:	Ingles English
Published:	2022-07-28
Subjects:	Leaf anatomy, False positive, Anatomia foliar, Duplo haploide, Falso positivo, Milho, Anatomia Vegetal, Zea Mays, Doubled haploids,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1145025 http://dx.doi.org/10.1590/1984-70332022v22n2a16
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