In vitro viability of pollen grain of fiji dwarf coconut accession.
Lethal yellowing is a disease that causes severe damages to coconut palms and it has devastated the coconut canopy of several Caribbean islands, and areas of southern Florida. The Fiji dwarf coconut accession has demonstrated tolerance to this disease. Pollen storage and germination provides useful information for conservation strategy and genetic improvement programs. The objective of this study was to evaluate pollen viability of Fiji dwarf coconut by in vitro germination under different nutritional and abiotic conditions. For the first experiment, two liquid culture media were evaluated for in vitro germination of Fiji dwarf coconut pollen; the Lora culture medium and the Armendariz culture medium combined with four temperature levels. In the second experiment, different sucrose concentrations in the Lora culture medium (4, 8, 12 and 16%) were evaluated combined with two incubation times (6 and 24 h) in the presence or absence of light. The highest in vitro germination of pollen grain was obtained in Lora culture medium by 24 h incubation period at 28 ºC. The light condition does not influence the germination process. In vitro germination percentage improved as the sucrose concentration increased according to a linear regression. In contrast, damage pollen grain percentage reduced according to a quadratic regression.
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Format: | Artigo de periódico biblioteca |
Language: | Ingles English |
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2021-12-14
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Subjects: | Germinação in vitro, Coco, Doença de Planta, Pólen, Germinação, Coconuts, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1137813 |
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dig-alice-doc-11378132021-12-15T10:47:11Z In vitro viability of pollen grain of fiji dwarf coconut accession. MACHADO, C. de A. VENDRAME, W. A. RUBIO NETO, A. CHAGAS, E. A. CHAGAS, P. C. LEDO, A. da S. CAROLINE DE ARAÚJO MACHADO, UFS; WAGNER APARECIDO VENDRAME, UNIVERSITY OF FLORIDA; AURÉLIO RÚBIO NETO, IFG; EDVAN ALVES CHAGAS, CPAF-RR; POLLYANA CARDOSO CHAGAS, UFRR; ANA DA SILVA LEDO, CPATC. Germinação in vitro Coco Doença de Planta Pólen Germinação Coconuts Lethal yellowing is a disease that causes severe damages to coconut palms and it has devastated the coconut canopy of several Caribbean islands, and areas of southern Florida. The Fiji dwarf coconut accession has demonstrated tolerance to this disease. Pollen storage and germination provides useful information for conservation strategy and genetic improvement programs. The objective of this study was to evaluate pollen viability of Fiji dwarf coconut by in vitro germination under different nutritional and abiotic conditions. For the first experiment, two liquid culture media were evaluated for in vitro germination of Fiji dwarf coconut pollen; the Lora culture medium and the Armendariz culture medium combined with four temperature levels. In the second experiment, different sucrose concentrations in the Lora culture medium (4, 8, 12 and 16%) were evaluated combined with two incubation times (6 and 24 h) in the presence or absence of light. The highest in vitro germination of pollen grain was obtained in Lora culture medium by 24 h incubation period at 28 ºC. The light condition does not influence the germination process. In vitro germination percentage improved as the sucrose concentration increased according to a linear regression. In contrast, damage pollen grain percentage reduced according to a quadratic regression. 2021-12-15T10:47:03Z 2021-12-15T10:47:03Z 2021-12-14 2021 Artigo de periódico Plant Cell Culture & Micropropagation, n. 17, e166, 2021. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1137813 Ingles en openAccess |
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Germinação in vitro Coco Doença de Planta Pólen Germinação Coconuts Germinação in vitro Coco Doença de Planta Pólen Germinação Coconuts |
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Germinação in vitro Coco Doença de Planta Pólen Germinação Coconuts Germinação in vitro Coco Doença de Planta Pólen Germinação Coconuts MACHADO, C. de A. VENDRAME, W. A. RUBIO NETO, A. CHAGAS, E. A. CHAGAS, P. C. LEDO, A. da S. In vitro viability of pollen grain of fiji dwarf coconut accession. |
description |
Lethal yellowing is a disease that causes severe damages to coconut palms and it has devastated the coconut canopy of several Caribbean islands, and areas of southern Florida. The Fiji dwarf coconut accession has demonstrated tolerance to this disease. Pollen storage and germination provides useful information for conservation strategy and genetic improvement programs. The objective of this study was to evaluate pollen viability of Fiji dwarf coconut by in vitro germination under different nutritional and abiotic conditions. For the first experiment, two liquid culture media were evaluated for in vitro germination of Fiji dwarf coconut pollen; the Lora culture medium and the Armendariz culture medium combined with four temperature levels. In the second experiment, different sucrose concentrations in the Lora culture medium (4, 8, 12 and 16%) were evaluated combined with two incubation times (6 and 24 h) in the presence or absence of light. The highest in vitro germination of pollen grain was obtained in Lora culture medium by 24 h incubation period at 28 ºC. The light condition does not influence the germination process. In vitro germination percentage improved as the sucrose concentration increased according to a linear regression. In contrast, damage pollen grain percentage reduced according to a quadratic regression. |
author2 |
CAROLINE DE ARAÚJO MACHADO, UFS; WAGNER APARECIDO VENDRAME, UNIVERSITY OF FLORIDA; AURÉLIO RÚBIO NETO, IFG; EDVAN ALVES CHAGAS, CPAF-RR; POLLYANA CARDOSO CHAGAS, UFRR; ANA DA SILVA LEDO, CPATC. |
author_facet |
CAROLINE DE ARAÚJO MACHADO, UFS; WAGNER APARECIDO VENDRAME, UNIVERSITY OF FLORIDA; AURÉLIO RÚBIO NETO, IFG; EDVAN ALVES CHAGAS, CPAF-RR; POLLYANA CARDOSO CHAGAS, UFRR; ANA DA SILVA LEDO, CPATC. MACHADO, C. de A. VENDRAME, W. A. RUBIO NETO, A. CHAGAS, E. A. CHAGAS, P. C. LEDO, A. da S. |
format |
Artigo de periódico |
topic_facet |
Germinação in vitro Coco Doença de Planta Pólen Germinação Coconuts |
author |
MACHADO, C. de A. VENDRAME, W. A. RUBIO NETO, A. CHAGAS, E. A. CHAGAS, P. C. LEDO, A. da S. |
author_sort |
MACHADO, C. de A. |
title |
In vitro viability of pollen grain of fiji dwarf coconut accession. |
title_short |
In vitro viability of pollen grain of fiji dwarf coconut accession. |
title_full |
In vitro viability of pollen grain of fiji dwarf coconut accession. |
title_fullStr |
In vitro viability of pollen grain of fiji dwarf coconut accession. |
title_full_unstemmed |
In vitro viability of pollen grain of fiji dwarf coconut accession. |
title_sort |
in vitro viability of pollen grain of fiji dwarf coconut accession. |
publishDate |
2021-12-14 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1137813 |
work_keys_str_mv |
AT machadocdea invitroviabilityofpollengrainoffijidwarfcoconutaccession AT vendramewa invitroviabilityofpollengrainoffijidwarfcoconutaccession AT rubionetoa invitroviabilityofpollengrainoffijidwarfcoconutaccession AT chagasea invitroviabilityofpollengrainoffijidwarfcoconutaccession AT chagaspc invitroviabilityofpollengrainoffijidwarfcoconutaccession AT ledoadas invitroviabilityofpollengrainoffijidwarfcoconutaccession |
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1756027901833117696 |